Human gal 3 quantikine elisa kit

The levels of Gal-3 in sera of PBC patients (11) and healthy controls (11) were determined using Human Gal-3 Quantikine ELISA Kit (R&D). Human liver biopsy samples were obtained from the Department of Pathology University of Kragujevac tissue collection and were stained with mouse antihuman Gal-3 antibody (Abcam, Cambridge, UK) and the mouse specific conjugate (Expose Ms-Specific HRP/DAB Detection IHC Kit; Abcam), according to manufacturer instructions.

For measurements of hemoglobin (HB), creatinine (CR), urea nitrogen (BUN), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), blood was collected from each patient after gaining the consent. These blood parameters were measured at the Central Clinical Laboratory of the First Affiliated Hospital of Xi'an Jiaotong University. HB was measured with automated cell counters via standard techniques by HST201 (Sysmex, Japan). CR, BUN, ALT and AST were examined by Beckman automatic biochemical analyzer AU5431 (America). After overnight fasting, between 6–7 a.m., blood from the median cubital vein was drawn into ethylenediaminetetraacetic acid (EDTA)-containing tubes, centrifuged at 4°C (3,000 rpm in 10 min) within 2 h after collection and stored at −80°C for later analyses (i.e., to detect Gal-3 and TIMP-1 levels).
Plasma Gal-3 levels and TIMP-1 were detected by a Human Gal-3 Quantikine ELISA Kit (R&D Systems Inc., Minneapolis, MN, USA) and a human TIMP-1 Quantikine ELISA Kit (Abbkine, Inc., China) in duplicates wells following protocols provided by the manufacturer, respectively. The mean plasma Gal-3 and TIMP-1 were calculated as the final level. The detection range of the plasma Gal-3 was 0–4,000 pg/mL. The calibration range of serum TIMP-1 was 31.25–2,000 ng/mL, and the limit of detection was 16 ng/mL.