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Mouse ephrin b1 fc chimera

Manufactured by R&D Systems
Sourced in United States

Mouse ephrin-B1/Fc Chimera is a recombinant protein consisting of the extracellular domain of mouse ephrin-B1 fused to the Fc region of human IgG1. It is a soluble, dimeric protein that can be used in research applications studying cell-cell interactions mediated by ephrin signaling.

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2 protocols using mouse ephrin b1 fc chimera

1

Ephrin-Fc receptor binding assay

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KYL (H2N-KYLPYWPVLSSL-COOH), biotinylated KYL (H2N-KYLPYWPVLSSLGSGSK-(biotin)-COOH), WDC (H2N-WDCNGPYCHWLG-COOH), and biotinylated WDC (H2N-WDCNGPYCHWLGGSGSK-(biotin)-COOH) were synthesized by BEX CO., LTD. (Tokyo, Japan). Recombinant ephrin-Fc were obtained from the companies as following: mouse ephrin-A1/Fc Chimera and mouse ephrin-B1/Fc Chimera from R&D Systems (MN, USA), ChromPure Human IgG Fc Fragment and A niPure goat anti-human IgG Fc fragment speci c from Jackson Immuno Research Laboratories (PA, USA). 100 μL of ephrin-Fc (100 μg/ mL) was mixed with 19 μL anti-human IgG and added to cells after 1 hr of incubation at 37℃.
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2

Ephrin-Fc receptor binding assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
KYL (H2N-KYLPYWPVLSSL-COOH), biotinylated KYL (H2N-KYLPYWPVLSSLGSGSK-(biotin)-COOH), WDC (H2N-WDCNGPYCHWLG-COOH), and biotinylated WDC (H2N-WDCNGPYCHWLGGSGSK-(biotin)-COOH) were synthesized by BEX CO., LTD. (Tokyo, Japan). Recombinant ephrin-Fc were obtained from the companies as following: mouse ephrin-A1/Fc Chimera and mouse ephrin-B1/Fc Chimera from R&D Systems (MN, USA), ChromPure Human IgG Fc Fragment and A niPure goat anti-human IgG Fc fragment speci c from Jackson Immuno Research Laboratories (PA, USA). 100 μL of ephrin-Fc (100 μg/ mL) was mixed with 19 μL anti-human IgG and added to cells after 1 hr of incubation at 37℃.
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