Hct116

Manufactured by Apexbio

Sourced in United States

The HCT116 is a cell line derived from human colorectal carcinoma. It is commonly used in cell biology and cancer research applications.

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Lab products found in correlation

Dld 1, by Thermo Fisher Scientific (3 mentions) Sw620, by Apexbio (3 mentions) Sr11302, by Apexbio (3 mentions) Ht 29, by Thermo Fisher Scientific (3 mentions) Hct116, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions) Sw620, by Keygen Biotech (3 mentions) L15 medium, by Keygen Biotech (3 mentions) Mccoy s 5a medium, by Keygen Biotech (3 mentions) Sw620, by National Collection of Authenticated Cell Cultures (3 mentions) Dld 1, by National Collection of Authenticated Cell Cultures (3 mentions) Hct116, by National Collection of Authenticated Cell Cultures (3 mentions) Sw480, by National Collection of Authenticated Cell Cultures (3 mentions) Dulbecco s modi ed eagle medium dmem, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)

3 protocols using hct116

Colorectal Cancer Cell Line Cultivation

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Human CRC cell lines (HCT116, SW620, SW480, DLD-1, HT-29) were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China). The human normal colorectal epithelial cell line FHC was obtained from the American Type Culture Collection (Manassas, VA, USA). Cell lines were cultured in the appropriate medium supplemented with 10% fetal bovine serum (FBS; Gibco, NY, USA) and 1% antibiotic/antimycotic solution and maintained in an incubator at 37 °C with 5% CO₂ in a humidified atmosphere. DLD-1 and HT-29 cells were maintained in RPMI-1640 medium (Gibco), FHC cells were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco), HCT116 cells were maintained in McCoy’s 5A medium (KeyGEN, Nanjing, China) and SW620 cells were maintained in L-15 medium (KeyGEN). To test the effects of the N-terminal phosphorylation of c-Jun, HCT116 and SW620 cells were pretreated with SR11302 (10 μM for 1 h) (APExBIO, USA) before transient transfection [30 ].

Yang T., Chen W.C., Shi P.C., Liu M.R., Jiang T., Song H., Wang J.Q., Fan R.Z., Pei D.S, & Song J. (2020). Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer progression by cis-regulating the nearby gene MK5 and acting as a let-7f-1-3p sponge. Journal of Experimental & Clinical Cancer Research : CR, 39, 139.

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Investigating c-Jun Phosphorylation in CRC Cells

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Human CRC cell lines (HCT116, SW620, SW480, DLD-1, HT-29) were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China). The human normal colorectal epithelial cell line FHC was obtained from the American Type Culture Collection (Manassas, VA, USA). Cell lines were cultured in the appropriate medium supplemented with 10% fetal bovine serum (FBS; Gibco, NY, USA) and 1% antibiotic/antimycotic solution and maintained in an incubator at 37°C with 5% CO 2 in a humidi ed atmosphere. DLD-1 and HT-29 cells were maintained in RPMI-1640 medium (Gibco), FHC cells were cultured in Dulbecco's modi ed Eagle medium (DMEM; Gibco), HCT116 cells were maintained in McCoy's 5A medium (KeyGEN, Nanjing, China) and SW620 cells were maintained in L-15 medium (KeyGEN). To test the effects of the N-terminal phosphorylation of c-Jun, HCT116 and SW620 cells were pretreated with SR11302 (10 μM for 1 h) (APExBIO, USA) before transient transfection [30] .

Yang T., Chen W., Shi P., Liu M., Jiang T., Song H., Wang J., Fan R., Pei D., & Song J. (2020). Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer progression by cis-regulating the nearby gene MK5 and acting as a let-7f-1-3p sponge.

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Investigating c-Jun Phosphorylation in CRC Cells

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Human CRC cell lines (HCT116, SW620, SW480, DLD-1, HT-29) were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China). The human normal colorectal epithelial cell line FHC was obtained from the American Type Culture Collection (Manassas, VA, USA). Cell lines were cultured in the appropriate medium supplemented with 10% fetal bovine serum (FBS; Gibco, NY, USA) and 1% antibiotic/antimycotic solution and maintained in an incubator at 37°C with 5% CO 2 in a humidi ed atmosphere. DLD-1 and HT-29 cells were maintained in RPMI-1640 medium (Gibco), FHC cells were cultured in Dulbecco's modi ed Eagle medium (DMEM; Gibco), HCT116 cells were maintained in McCoy's 5A medium (KeyGEN, Nanjing, China) and SW620 cells were maintained in L-15 medium (KeyGEN). To test the effects of the N-terminal phosphorylation of c-Jun, HCT116 and SW620 cells were pretreated with SR11302 (10 μM for 1 h) (APExBIO, USA) before transient transfection [30] .

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