Cortisol, cortisone and dehydroepiandrosterone (DHEA) were purchased form Koch-Light Laboratories LTD (Colnbrook, Great Britain),
7α-OH-DHEA, 7β-OH-DHEA,
7-oxo-DHEA,
16α-OH-DHEA and
D3-DHEA were from Steraloids (Newport, USA).
D4-Cortisol was from CDN isotopes (Ponte-Claire, Canada).
2-hydrazinopyridine,
ammonium formate,
methyl tertbutyl ether and
trifluoroacetic acid were from Sigma-Aldrich (St. Louis, USA).
LC-MS grade methanol, water and
diethyl ether were from Merck AG (Darmstadt, Germany). The physiological solution (0.9 % sodium chloride) was from B-Braun (Melsungen AG, Germany). [1,2,6,7-3H]Cortisol, specific radioactivity 3.04 TBq/mmol was from Amesrham Biosciences, Inc.
(Amersham, UK).
Máčová L., Sosvorová L., Vítků J., Bičíková M., Hill M., Zamrazilová H., Sedláčková B, & Stárka L. (2015). Steroid hormones related to 11beta-hydroxysteroid dehydrogenase type 1 in treated obesity. Physiological research, 64(Suppl 2).