Dna gel loading dye 6

Materials. Pirarubicin, superoxide dismutase (SOD; 3,000 U/mg from bovine erythrocytes), catalase (45,000 U/mg from bovine liver) and cytochrome c (from equine heart) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Plasmid DNA (pBR322) and DNA gel loading dye (6×) were from Toyobo Co. (Osaka, Japan). Copper chloride (CuCl 2 •2H 2 O) was from Nacalai Tesque Co (Kyoto, Japan). Diethylenetriamine-N,N,N',N'',N''-penta-acetic acid (DTPA) and bathocuproinedisulfonic acid were from Dojin Chemicals Co. (Kumamoto, Japan). 3-(Methylthio) propionaldehyde (methional) was from Tokyo Kasei Co. (Tokyo, Japan). All other chemicals used were of the highest purity commercially available.

Materials. Aclarubicin hydrochloride was purchased from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan). Superoxide dismutase (SOD; 3,000 U/mg from bovine erythrocytes), catalase (45,000 U/mg from bovine liver) and cytochrome c (from equine heart) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Plasmid DNA (pBR322) and DNA gel loading dye (6×) were from Toyobo Co. (Osaka, Japan). Copper chloride (CuCl 2 •2H 2 O) Cell culture and treatment with ACR. Human leukemia HL-60 and HP100 cells were obtained from Riken BioResource Research Center (Tsukuba, Japan). HP100 cells have approximately 340-fold higher resistance to H 2 O 2 (14) (link) and 18 times higher catalase activity (15) than HL-60 cells. By use of HP100 cells, effects of intracellular catalase can be evaluated (16) (link). HL-60 and HP100 cells were grown in RPMI 1640 (FUJIFILM Wako Pure Chemical Co.) supplemented with 6% fetal bovine serum (Biowest Co., Nuaillé, France) at 37˚C under 5% CO 2 in a humidified atmosphere. The cells (0.5×10 6 or 1×10 6 cells/ml) were then treated with 0.05, 0.1, 0.2 and 0.5 μM of ACR.