All the lipids, including 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG2000-MAL), a mini extruder, and polycarbonate membrane filters were purchased from Avanti Polar Lipids Inc. Cholesterol, protamine, and chloroform were purchased from Sigma-Aldrich. Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), SYBR Safe, and lipofectamine 2000 were purchased from Invitrogen.
1 2 distearoyl sn glycero 3 phosphoethanolamine n maleimide polyethylene glycol 2000 dspe peg2000 mal
Manufactured by Avanti Polar Lipids
Sourced in United States
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (DSPE-PEG2000-MAL) is a lipid conjugate that contains a maleimide functional group and a polyethylene glycol (PEG) spacer. The maleimide group can be used for covalent attachment to thiol-containing molecules.
Automatically generated - may contain errors
Lab products found in correlation
Protamine, by Merck Group (1 mentions)
Sybr safe, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine dope, by Avanti Polar Lipids (1 mentions)
Cholesterol, by Merck Group (1 mentions)
1 2 dioleoyl 3 trimethylammonium propane, by Avanti Polar Lipids (1 mentions)
Anti mouse cd3, by BioLegend (1 mentions)
Anti mouse cd11c n418, by BioLegend (1 mentions)
Tween 20, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Cytochrome c, by Santa Cruz Biotechnology (1 mentions)
Caspase 9, by Santa Cruz Biotechnology (1 mentions)
Caspase 3, by ABclonal (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Cholesterol, by Avanti Polar Lipids (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Tcep reducing agent, by Merck Group (1 mentions)
Kieselgel 60 f254, by Merck Group (1 mentions)
5 protocols using 1 2 distearoyl sn glycero 3 phosphoethanolamine n maleimide polyethylene glycol 2000 dspe peg2000 mal
1
Liposomal Formulation Development
2
Liposomal Bufalin Delivery for Anti-CD40 Therapy
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L-α-phosphatidylcholine (EPC), cholesterol, dithiothreitol (DTT), and Tween 20 were purchased from Sigma-Aldrich (St Louis, MO, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] (DSPE-PEG2000-Mal), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (ammonium salt; DSPE-PEG2000), and rhodamine-phosphoethanolamine (Rho-PE) were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Bufalin (purity >98%) was purchased from BaoJi ChenGuang Technology Development Co. Ltd (BaoJi, Shaanxi, People’s Republic of China). Monoclonal anti-CD40 (clone FGK4.5, rat IgG2a) was purchased from Bio X Cell (West Lebanon, NH, USA). Zeba desalting columns were obtained from Pierce (Thermo Fisher Scientific, Rockford, IL, USA). Secondary antirat IgG was purchased from Jackson ImmunoResearch Labs (West Grove, PA, USA). Anti-CD40 enzyme-linked immunosorbent assay (ELISA) kits were purchased from Blue Gene Technology Development Co. Ltd (Shanghai, People’s Republic of China). Antimouse CD45 (clone 30-F11), antimouse CD4, antimouse CD3, antimouse CD8a, and antimouse CD11c (N418) were obtained from Biolegend (San Diego, CA, USA). Cytochrome c and caspase-9 were obtained from Santa Cruz Biotechnology Co., Ltd (Dallas, TX, USA), and caspase-3 was obtained from ABclonal Biotech Co., Ltd (Cambridge, MA, USA).
3
Multifunctional Theranostic Nanoparticles
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1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)2000] (DSPE-mPEG2000), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethyleneglycol)2000] (DSPE-PEG2000-MAL), and cholesterol were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). O,O′-Dimyristyl-N-lysyl glutamate (DMKE) cationic lipid was chemically synthesized by Dr. D.O. Jang (Yonsei University, Wonju, Korea)18 (link). 5′-Epidermal growth factor receptor (EGFR) aptamer-SH-3′ was purchased from Aptamer Science Inc. (Pohang, Korea). CdSe/ZnS High Quality Organic NSQ-dots quantum dots (Q-dots, λemit = 620 nm) were purchased from Nanosquare Inc. (Seoul, Korea). AccuTarget™ fluorescein-labeled siRNA and negative control siRNA were purchased from BioNeer Inc. (Daejeon, Korea).
4
Liposomal Delivery of Atorvastatin and Curcumin
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Cholesterol (Chol, Mw: 386.65) in powder form, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC, Mw: 734.05), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(maleimide(polyethylene glycol)2000) (Mal-PEG2000-DSPE, Mw: 2893) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Ato and Cur were purchased from Sigma-Aldrich (St Louis, MO, USA). E-selectin-binding (Esb) peptide (sequence: H2N-DITWDQLWDLMK-COOH) was synthesized by Minghao Biotech Inc. Co. (Wuhan, China). Flow cytometer and real-time mRNA quantification kits were purchased from KeyGen Biotech In. Co. (Nanjing, China) and Roche Life Science (Basel, Switzerland), respectively. The fluorescent probe, DAPI, was from Beyotime (Shanghai, China).
5
Synthesis of Peptide-PEG-Lipid Conjugates
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The CD177-binding dodecapeptide lipid was prepared by first exposing 1.0 ml of an 8.8 mg/ml aqueous solution of cysteine-terminated dodecapeptides (Peptide H, Peptide M or scrambled) to resin-supported TCEP reducing agent (Sigma-Aldrich) by shaking for 15 minutes. The solution was filtered directly into a flask containing 17.6 mg of powdered 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (“mal-Peg2000-DSPE”) (Avanti Polar Lipids, Alabaster, Alabama) and stirred under nitrogen. The powdered DSPE lipid dissolved completely after several hours and was allowed to react overnight at ambient temperature. Thin layer chromatography (TLC) analysis on silica gel plates (Kieselgel 60 F254, Merck KGaA, Darmstadt, Germany) with chloroform/methanol solvent mixture (4/1) as eluent showed that all the mal-Peg2000-DSPE was consumed and converted to a lower Rf spot. The solution was dried to a waxy solid and redissolved in 1/1 chloroform/methanol and filtered through Celite yielding Peptide H, Peptide M or scrambled peptide-Peg2000-DSPE.
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