Phospholipid, 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (ammonium salt), L-α-phosphotidylglycerol sodium salt (FITC-PC) and PC 16:0/18:1 was commercially obtained (Avanti Polar Lipids, Inc., Alabaster, Al). Cholesterol was purchased from Calbiochem (San Diego, CA, USA). Liposome formulations were prepared by lipid film hydration and sonication methods as described previously (Mfuh et al. 2011 (link); Li et al. 2012a (link), b (link)). Liposome encapsulated PC 16:0/18:1 or liposomes alone were introduced into Hela and 104C1 cells in triplicate at a concentration of 25 µM/well. Following centrifugation at ~1000×g for 60 min at 25 °C, cells were washed with D-10 and infected with Ct D as described above.
Pc 16 0 18 1
Manufactured by Avanti Polar Lipids
Sourced in United States
PC 16:0/18:1 is a polar lipid product offered by Avanti Polar Lipids. It is a synthetic phosphatidylcholine (PC) molecule with a 16:0 (palmitoyl) fatty acid chain on the sn-1 position and an 18:1 (oleoyl) fatty acid chain on the sn-2 position.
Automatically generated - may contain errors
Lab products found in correlation
N hexane, by Merck Group (1 mentions)
Milli q water system, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
2 propanol, by Thermo Fisher Scientific (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Cacl2, by Merck Group (1 mentions)
Methanol meoh, by Biosolve (1 mentions)
Pc16 0 18 2, by Avanti Polar Lipids (1 mentions)
Peth 16 0 18 1, by Avanti Polar Lipids (1 mentions)
Peth 16 0 18 2, by Avanti Polar Lipids (1 mentions)
2 protocols using pc 16 0 18 1
1
Liposome-Mediated Delivery of Phospholipids
2
Mass Spectrometry Analysis of Lipid Standards
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PEth 16:0/18:1, PEth 16:0/18:2, PC 16:0/18:1, and PC 16:0/18:2 were obtained from Avanti Polar Lipids (Alabaster, USA). PLD and n-hexane were from Sigma-Aldrich (Buchs, Switzerland). Acetone, ammonium acetate, chloroform (CHCl 3 ), D 6 -ethanol, diethyl ether, CaCl 2 , and Na 2 HPO 4 were obtained from Merck (Darmstadt, Germany). 2-Propanol was provided by Fisher Scientific (Loughborough, UK), methanol (MeOH) was from Biosolve BV (Valkenswaard, the Netherlands), and acetonitrile (MeCN) was supplied by Agros Organics (New Jersey, USA). Deionized water was produced in-house with a Milli-Q water system from Millipore (Billerica, USA). Blank blood was obtained from the blood donation center in Bern, Switzerland.
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