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Vhx digital microscope

Manufactured by Keyence
Sourced in Japan

The VHX digital microscope is a versatile imaging and measurement tool. It captures high-resolution images and videos of samples through a digital camera and advanced optics. The VHX provides accurate measurement capabilities and can be used to inspect a wide range of materials and surfaces.

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12 protocols using vhx digital microscope

1

Characterization of Electrospun Fibrous Mats

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The morphology of all the fibrous mats was characterized using Field-Emission Scanning Electron Microscopy (SEM, Supra 40, Carl Zeiss, Germany). Specimens were sputter-coated with Ag-Pd alloy using a sputter coater (Q150T, Carl Zeiss, Germany) to improve the electrical conductivity, then observed using SEM at a voltage of 8 kV and 1500 X magnification. ImageJ software (ImageJ, Version 1.53b, National Institute of Health, Bethesda, MD, USA) was used to assess the MFD based on the obtained SEM images. Mean fiber diameter was calculated as an average of fiber diameters measured at 30 different positions on each SEM image of the specimen.
For the mechanical testing, all specimens were prepared to have a width of 13 mm and further taped at both ends to prevent gripping failures. The VHX Digital Microscope (Keyence Corp, Osaka, Japan) was used to precisely record the thickness (n ≥ 5) of the electrospun membrane without any compression by mechanical instrumentation. Uniaxial tensile testing was carried out according to ASTM D882 Standard test method using a Sintech 2/G Materials Testing System (MTS, Eden Prairie, MN, USA) at an extension rate of 0.5 in/min as depicted in Figure 6a,b. Mechanical properties such as elastic modulus and TS were calculated using MTS Testworks 4.0 software (MTS, Eden Prairie, MN, USA).
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2

Microstructural Characterization of Alloy Samples

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Macroscopic images were taken using a VHX digital microscope (Keyence, Osaka, Japan). The microstructure was investigated with a Scanning Electron Microscope EVO MA 25 (Zeiss, Oberkochen, Germany). The samples in the form of plates were prepared for SEM analysis by grinding on abrasive from #200 to #2000 and polishing using colloidal SiO2 to obtain a mirror surface and then etched with Kroll reagent (92 mL distilled water + 6 mL nitric acid + 2 mL hydrofluoric acid). Phase analysis was performed using an X-ray powder diffractometer MiniFlex 600 (Rigaku, Tokyo, Japan) and Smart Lab Studio II software (Rigaku, Tokyo, Japan). XRD was conducted using CuKα radiation and a scanning speed of 2°/min from 30° to 90°. Square surfaces (20 mm × 20 mm) were polished using #2000 abrasive and colloidal SiO2 with polishing cloth to ensure that the diffractometry was performed in the interior structure.
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3

Fiber Diameter Analysis Using VHX Microscope

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Images of the cBSA- and BSA-fibers were taken with a VHX Digital microscope (Keyence, Osaka, Japan). For the analysis with the software VHX 5000 (Version 1.5.1.0) 10 images of each fiber type from 3 different batches were taken. We measured the diameter of 5 different fibers in one image. In total 150 data points were generated for cBSA and BSA each.
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4

Tensile Properties of ELP-Collagen Composites

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The dog-bone shaped specimens (n ≥ 6) were kept wet by immersion in PBS at 37°C and >70% humidity until they were tested on day 8. The dimensions of the specimens were measured using VHX Digital Microscope (Keyence Corp, Osaka, Japan) and Vernier calipers. Average thicknesses of the H2 and H4 ELP-collagen composites were 0.84 ± 0.30 and 1.47 ± 0.45 mm, respectively and were not statistically different (p > 0.05) from their respective H1 and H3 control collagen hydrogels (0.76 ± 0.33 and 1.54 ± 0.45, respectively). Average widths of the H1, H2, H3, and H4 composites were 3.25 ± 0.53, 2.89 ± 0.61, 3.32 ± 0.35, and 3.49 ± 0.34 mm, respectively. As expected, the widths of all composites were not statistically different (p > 0.05) since they were prepared in the custom-made, dog-bone shaped acrylic mold having precise width. Uniaxial testing was performed with ASTM D882 on a Sintech 2/G Materials Testing System (MTS, Eden Prairie, MN, USA) at an extension rate of 0.5 in/min (12.7 mm/min). Tensile strength, elastic modulus , and toughness were calculated using MTS Testworks 4.0 software.
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5

Isolation and Characterization of Fungal Metabolites

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To confirm the AF productivity of the FUT-A1 strain and other fungi isolated from soils of Fukui city, Japan, we cut out an aliquot of the fungal colony together with the agar underneath the colony on an agar plate and transferred it to a new 1.5 mL microtube. Metabolites in the samples were extracted with 0.3 mL of ethyl acetate followed by mixing with a vortex mixer. After centrifugation at 10,000× g for 2 min, the upper extract (10 μL) was analyzed by TLC using a silica gel plate (silica gel 60, #5721; Merck, Rahway, NJ, USA) and a developing solution of toluene-ethyl acetate-acetic acid (60:30:4, v/v/v).
For the determination of the morphological characters, each fungus was inoculated on PDA medium and cultured for 4 days. The colony was observed using a VHX digital microscope equipped with a VH-Z25 lens (Keyence, Osaka, Japan).
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6

Quantifying Cellular Oil and DNA Content

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DNA content (Cyquant, ThermoFisher Scientific, Waltham, MA, USA) and Oil Red O (Sigma, St. Louis, MO, USA) assays were preformed according to manufacturers’ protocols (n = 3). Cells were imaged using an IX-81 microscope (Olympus, Center Valley, PA, USA). Cells stained with Oil Red O were imaged using a VHX digital microscope (Keyence Corp., Osaka, Japan). For quantitative determination of Oil Red O staining, the stain was extracted using isopropanol and absorbance was measured at 405 nm using ELX-800 absorbance plate reader (Winooski, VT, USA). To measure DNA content, cells were first removed from the scaffolds using a collagenase I solution (Sigma) and then lysed using a Branson Digital Sonifier 450 (Danbury, CT, USA).
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7

Cellular Analysis of Leaf Segment Cultures

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To observe cell division and SAM formation on the cultured leaf segments of “Kaname,” “Concord,” and “Monique,” the cultured samples were fixed in a formalin-acetic acid-alcohol (FAA) solution (ethanol: formaldehyde: acetic acid: water = 12:1:1:6 v/v/v/v) after 0–18 d of culture and evacuated for several days in FAA. Then, the samples were washed with tap water and dehydrated through a series of different ethanol concentrations. Ethanol in the samples was replaced by a resin solution (Technovit 7100, Heraeus Kulzer, Germany) and then embedded in Technovit 7100 at 25°C according to the manufacturer's instructions. The samples were cut into 5 μm-thick sections using a microtome (RM2155, Leica Microsystems, Germany) and the sections were placed onto glass slides. Sections were then stained with 0.05% (w/v) toluidine blue and observed under a VHX digital microscope (Keyence, Japan). The number of cells and total cell number were counted in each epidermal layer and subepidermal layer within seven 2 × 2 mm randomly selected sections 0–14 d after culture for the three selected samples.
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8

Scanning Electron Microscopy of Sensor Platforms

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Scanning electron microscopy (SEM) images were recorded employing a Hitachi SU-70 SEM (Hitachi, Tokyo, Japan) using an accelerating voltage of 5 keV for SEM images. Samples coated onto Si wafers were cleaved and sputter coated with 6.1 nm of platinum/palladium coating using a Cressington 208HR sputter coater (Cressington, Whatman, UK) to reduce the charge of the samples. Optical microscopy images of the sensor platform were imaged using a Keyence VHX digital microscope (Keyence, Milton Keynes, UK).
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9

Histological Examination of Hyperolius Skin

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In a first step, all preserved specimens and digital photos of Hyperolius spp. were examined for the presence of skin efflorescences to estimate the rate of affected individuals per species. Independently of externally visible skin lesions, samples of all preserved specimens were histologically examined for pathological dermal modifications. To achieve an overview of external skin abnormalities, photos were taken at magnifications of 20 to 200× using a Keyence VHX digital microscope. Skin lesions and prominent structures of the heavily infected H. lateralis (ZFMK 96782) were excised with a sterile scalpel, embedded in paraffin, sectioned, stained with periodic acid-Schiff (PAS) following standard techniques, and examined with higher magnification and phase contrast with a Leica Stemi 2000C microscope equipped with a Hitachi KP-FD140F-S3 camera. The micrographs at higher magnification were taken with a Leica DM5000 B fluorescence microscope equipped with a JVC 3CCD 3-chip camera. The software used for documentation purposes was DISKUS 4.30 (Hilgers).
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10

Histochemical GUS Assay in Transgenic Arabidopsis

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Transgenic Arabidopsis plants carrying the mot::gus constructs were grown hydroponically under molybdate availability conditions for 60 days. Positional effects of the integration locus of the constructs were avoided by use of several independently transformed lines. Plants were infiltrated with GUS-staining solution (9.6 mL 100 mM Tris/50 mM NaCl buffer pH 7.0, 0.2 mL 100 mM ferricyanide solution in Tris/NaCl buffer, 0.2 mL 100 mM X-Gluc solution in DMSO) according to Beeckman and Engler [74 (link)] using a vacuum chamber. Completely infiltrated tissue was incubated over night at 37 °C in the dark with a sufficient supply of oxygen. Subsequently, chlorophyll was extracted from the tissue using ethanol with concentrations of 30% (v/v), 60 % (v/v) and 96% (v/v) over a time span of 24 h each to allow a clear view of the coloured organs and tissue. Samples were kept in 96% (v/v) until photo documentation using a Keyence VHX digital microscope (Keyence, Osaka, Japan). Wild type control was free of background colouration (Figure S1).
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