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Anti βii tubulin

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Anti-βII-tubulin is a primary antibody used in immunological techniques to detect and quantify the presence of β-tubulin isoform II, a key structural component of microtubules. It provides a specific and reliable method for visualizing and analyzing the expression and distribution of this important cytoskeletal protein.

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Lab products found in correlation

Anti βiii tubulin, by Merck Group (2 mentions) Imagequant tl, by GE Healthcare (1 mentions) Anti gapdh, by Abcam (1 mentions)

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Anti-Tubulin (4 citations)

2 protocols using anti βii tubulin

1

Tubulin Isoforms Quantification by Western Blot

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Western blot analysis was performed using the following antibodies: anti-βIII-tubulin (Chemicon), anti-βII-tubulin (Covance), anti-βI-tubulin (Covance) and anti-GAPDH (Abcam) as described [22 (link), 24 (link), 28 (link)]. The blots were scanned using LAS4000 scanner and quantified using ImageQuant TL (GE Healthcare).
McCarroll J.A., Sharbeen G., Liu J., Youkhana J., Goldstein D., McCarthy N., Limbri L.F., Dischl D., Ceyhan G.O., Erkan M., Johns A.L., Biankin A.V., Kavallaris M, & Phillips P.A. (2014). βIII-Tubulin: A novel mediator of chemoresistance and metastases in pancreatic cancer. Oncotarget, 6(4), 2235-2249.
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2

Immunohistochemistry of Human and Mouse Tumor Tissue

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Human PDA tissue specimens were collected by surgical removal. The use of these sections was approved by UNSW Human Research Ethics Committee (HCEC# HC14039). Immunohistochemistry was performed on paraffin-embedded human or mouse tumor tissue sections as described [24 (link), 41 (link), 43 (link)]. Antibodies used were, anti-βIII-tubulin (1:200) (Chemicon), anti-βII-tubulin (1:200) (Covance), anti-luciferase (1:50) (Biovision incorporated).
McCarroll J.A., Sharbeen G., Liu J., Youkhana J., Goldstein D., McCarthy N., Limbri L.F., Dischl D., Ceyhan G.O., Erkan M., Johns A.L., Biankin A.V., Kavallaris M, & Phillips P.A. (2014). βIII-Tubulin: A novel mediator of chemoresistance and metastases in pancreatic cancer. Oncotarget, 6(4), 2235-2249.
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