HbA1c was analyzed with a point-of-care analyzer (Afinion AS100; Axis-Shield, Oslo, Norway) during the first study visit. All other blood and medium samples were analyzed at the Clinical Chemistry Laboratory, Sahlgrenska University Hospital, which is accredited in accordance with the International Standard ISO 15189:2007. ELISA was used to measure adiponectin (human adiponectin ELISA kit, EZHADP-61K, Millipore, Billerica, MA; interassay coefficient of variation (CV) 7.0% at 10.5 mg/l), TNF-α (human TNF-alpha Quantikine HS ELISA, HSTA00D, R&D Systems, Minneapolis, MN; interassay CV 6.0% at 2 ng/l), AFABP (human adipocyte FABP ELISA kit, RD191036200R, BioVendor Research and Diagnostic Products, Bratislava, Slovakia; inter-assay CV 10.0% at 16 μg/l) and leptin (human leptin Quantikine, DLP00, R&D Systems; interassay CV 8.0% at 9 μg/l). Glycerol was analyzed with a Randox radiometric glycerol kit (GY105; Crumlin, UK; interassay CV 4.0% at 0.1 μmol/l) and a Konelab 30 autoanalyzer (Thermo Clinical Labsystems, Vantaa, Finland). All other assays were routinely done with a Cobas Modular system (Roche Diagnostics, Mannheim, Germany).
Human adipocyte fabp elisa kit
Manufactured by BioVendor
Sourced in Mongolia, United States
The Human adipocyte FABP ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of human adipocyte fatty acid-binding protein (FABP) levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cobas modular system, by Roche (2 mentions)
Ezhadp 61k, by Merck Group (1 mentions)
Afinion as100, by Axis-Shield (1 mentions)
Human leptin ria kit, by Merck Group (1 mentions)
Quantikine hs elisa, by R&D Systems (1 mentions)
Human leptin quantikine, by R&D Systems (1 mentions)
Human adiponectin elisa kit, by Merck Group (1 mentions)
2 protocols using human adipocyte fabp elisa kit
1
Analytical Techniques for Biomarker Quantification
2
Maternal Adipokines and Gestational Diabetes
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All blood and medium samples were analyzed at the Clinical Chemistry Laboratory, Sahlgrenska University Hospital, accredited in accordance with the International Standard ISO 15189:2007. Enzyme-linked immunosorbent assay (ELISA) was used to measure adiponectin (Human adiponectin ELISA kit, Millipore, Billerica, MA, USA), tumor necrosis factor (TNF)-α (human TNF-alpha Quantikine HS ELISA, R&D Systems, Minneapolis, MN, USA) and adipocyte fatty acid-binding protein (AFABP) (Human Adipocyte FABP ELISA kit, BioVendor Research and Diagnostic Products, Bratislava, Slovakia). As a result of a change in assay during the study period, leptin was measured by RIA or ELISA (Human Leptin RIA kit, Millipore; Quantikine Human Leptin, R&D Systems; R=0.962). Samples from each woman in T1 and T3 were analyzed with the same leptin assay. HbA1c was measured by Mono-S ion-exchange chromatography. Glycerol was analyzed with a Randox radiometric glycerol kit (GY105; Crumlin, UK) and a Konelab 30 autoanalyzer (Thermo Clinical Labsystems, Vantaa, Finland). All other assays were routinely performed with a Cobas Modular system (Roche Diagnostics, Mannheim, Germany).
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