The LF-NMR relaxation measurements were carried out using a low field NMR analyzer (MesoQMR23-060H, Niumag Electric Corporation, Shanghai, China). The transverse relaxation time (T2) was measured using the Carr–Purcell–Meiboom–Gill (CPMG) sequence.
Mesoqmr23 060h
Manufactured by Niumag
Sourced in China
The MesoQMR23-060H is a laboratory equipment product designed for specific laboratory applications. It offers core functionality to perform relevant tasks within a controlled laboratory environment. The description of the product's intended use or any interpretation of its capabilities is not provided.
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7 protocols using mesoqmr23 060h
1
Low-field NMR Relaxation Measurements
2
LF-NMR Relaxation Time Analysis
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LF-NMR tests were carried out using an instrument (MesoQMR23-060H, Niumag Electric Corporation, Shanghai, China). About 2 g of the sample was packed into a glass tube with a diameter of 15 mm and inserted into the instrument. The tests were operated at 25 °C. The spin-spin relaxation time (T2) was recorded using the sequence of Carr–Purcell–Meiboom–Gill (CPMG). Other parameters were set as SW = 100 kHz, SF = 21 MHz, RG = 20 dB, TW = 4000 ms, NS = 4, TE = 0.2 ms and NECH = 18,000.
3
LF-NMR and MRI Analysis of Mixture
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LF-NMR measurements were performed with an analyzer (MesoQMR23-060H, Niumag Electric Corporation, Shanghai, China). Approximately 2 g of the mixture was placed in a cylindrical glass tube (15 mm diameter) and inserted into the NMR probe. The analyzer operated at 25 °C with a resonance frequency of 21 MHz. The transverse relaxation time (T2) was measured using the Carr–Purcell–Meiboom–Gill (CPMG) sequence with the following parameters: SW = 100 kHz, RG = 20 dB, NECH = 18,000, TE = 0.2 ms, NS = 4, TW = 4000 ms. Parameters relating to T2 are presented as follows: T2b, T21, and T22 are the relaxation components, while P2b, P21, and P22 are the corresponding area fractions.
In addition, magnetic resonance imaging (MRI) measurements were obtained by MSE sequence with the following parameters: FOV read = 60 mm, FOV phase = 60 mm, averages = 2, TR = 500 ms, TE = 20 ms.
In addition, magnetic resonance imaging (MRI) measurements were obtained by MSE sequence with the following parameters: FOV read = 60 mm, FOV phase = 60 mm, averages = 2, TR = 500 ms, TE = 20 ms.
4
Analyzing Water Distribution in Semi-Dried Noodles
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Refer to the method of Ge et al.—with a small modification [10 (link)]. The water distribution of semi-dried noodles was determined via a nuclear magnetic resonance imaging analyzer (MesoQMR23-060H, Niumag Electric Corporation, Shanghai, China). About 7 g of samples of about 3 cm in length were placed in the NMR tube and sealed with cling film to prevent moisture evaporation. Parameters measured were SW of 200 kHz, TW of 100 ms, TD of 24022, NS of 8 and NECH of 600.
5
Hind Leg Muscle and Body Composition Analysis
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Ten mice from each group were randomly selected for the measurement of the hind leg muscle circumference. Serum albumin levels were quantified in the same mice using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems Europe, Abingdon, UK) according to the manufacturer’s instructions. Meanwhile, five mice from each group were randomly selected for a body composition analysis using the MesoQMR23-060H nuclear magnetic resonance analyzer (Suzhou Niumag Analytical Instrument Corporation, Suzhou, China) with the following parameters: coil diameter, 60 mm; magnet strength, 0.5 T; resonance frequency, 21 MHz; and magnet temperature, 32 ± 0.01 °C.
6
Proton Distribution in Fresh Fibers
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1H proton signal distribution in fresh fibers was measured with a LF-NMR analyzer (MesoQMR23-060H, Niumag Electric Corporation, Shanghai, China) using the method [21 (link)] modified a little bit. Floating water on the fiber bundle surface needed to be removed with filter paper before testing. A 2 g sample was wrapped in polytetrafluoroethylene bags and placed inside a 15-mm diameter glass tube. In this experiment, the analyzer’s operating temperature was 32 °C, and its resonance frequency was 21 MHz. The transverse relaxation time (T2) was determined using the Carr-Purcell-Meiboom-Gill (CPMG) sequence with the following parameters: SW = 100 kHz; RG = 20 dB; NECH = 3000; TE = 0.2 ms; NS = 4, TW = 2000 ms.
7
Characterizing CL316243-Induced Body Composition
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After treatment with CL316243 for 4 weeks, the lean and fat content of mice was detected by Small Animal Body Composition Analysis and Imaging System NMR Analyzer (MesoQMR23-060H, Niumag Corp., Shanghai, China). Then, the iBAT temperature of mice under an environmental temperature of 25
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