Poloxamer

COS (molecular weight, 3.5 × 10³ Da) was purchased from Zhejiang Jinke Pharmaceutical Co., Ltd (Hangzhou, China). Poloxamer was obtained Germany BASF Corporation (Ludwigshafen, Germany). Reference standard aFGF was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). aFGF stoste (5 mg/ml, 2.34 × 10⁷ IU/ml, NO. 20150429) was synthesized in our laboratory. An aFGF enzyme-linked immunosorbent assay kit was purchased from Wuhan Huamei Biotechnology Co., Ltd (Wuhan, China). Fetal bovine serum (FBS), trypsin, and Dulbecco's modified essential medium (DMEM) were purchased from America Gibco Corporation (New York, USA). An electronic BW-YLS-3E instrument was purchased from Shanghai Ruanlong Technology Co., Ltd (Wuhan, China). A LE7406 hot plate analgesia meter was purchased from America Harvard Apparatus Corporation (USA). RSC96 cells were purchased from the Chinese Academy of Sciences (Shanghai, China).
Dimethylformamide was provided from Shanghai Demao Chemical Co., Ltd (Shanghai, China). Chlorosulfonic acid was purchased from Hubei Jusheng Technology Co., Ltd (Wuhan, China). Absolute ethyl alcohol, sodium hydrate, and Na₂S₂O₄ were obtained from Guangzhou Youyi Chemical Co., Ltd (Guangzhou, China). All reagents were analytical grade.