Blue mussel (Mytilus edulis) was purchased from Yeosu Fisheries Co. (Yeosu, Korea). Calcein AM solution, propidium iodide (PI), 2′7′-dichlorofluorescein diacetate (DCFH-DA), H2O2, Hoechst 33342, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Trypsin-EDTA solution and PBS were purchased from Hyclone (Logan, UT, USA). The HUVEC (PCS-100-010™) are produced by the American Type Culture Collection (Rockville, MD, USA). Endothelial Growth Medium-2 Bullet Kit (EGM™-2) was produced by Lonza, (Walkersville, MD, USA).
Huvec pcs 100 010
Manufactured by American Type Culture Collection
Sourced in United States
HUVEC (PCS-100-010™) is a primary cell line derived from human umbilical vein endothelial cells. It is intended for use in the study of endothelial cell biology.
Automatically generated - may contain errors
Lab products found in correlation
Endothelial growth medium 2 bullet kit egm 2, by Lonza (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Calcein am solution, by Merck Group (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
2 protocols using huvec pcs 100 010
1
Mussel-Derived Endothelial Cell Assays
2
Hydrogel-based Tissue Engineering Scaffolds
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Sodium alginate (viscosity = 200 ± 20 MPa), anhydrous calcium chloride, and magnesium chloride hexahydrate were purchased from the Aladdin Industrial Corporation (Shanghai, China). According to our previous study, HACC with a 26 % DS of quaternary ammonium was produced by combining chitosan and glycidyl trimethylammonium chloride (GTMAC) [32 (link)]. Chitosan (molecular weight = 5.0 × 104 g/mol, 87 % N-deacetylation) was purchased from Zhejiang Yuhuan Ocean Biochemistry Co., Ltd., China. N-Hydroxysuccinimide (NHS), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl), 1,4-dioxane, and GTMAC were purchased from Sigma–Aldrich, USA. Human dermal fibroblasts (HDFs; PCS-201-012) and HUVEC (PCS-100-010) were obtained from the American Type Culture Collection (ATCC), while human keratinocyte cells (HaCaT) (ACC-771) cells was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ).
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