Nadph

Manufactured by Yeasen

Sourced in China

NADPH is a cofactor essential for various enzymatic reactions in biological systems. It serves as a reducing agent and plays a crucial role in cellular metabolism, energy production, and antioxidant defense mechanisms.

Automatically generated - may contain errors

Lab products found in correlation

Genomic dna extraction kit, by Transgene (1 mentions) E coli bl21 de3, by Transgene (1 mentions) Plasmid miniprep kit, by Transgene (1 mentions) Fastpfu dna polymerase, by Transgene (1 mentions)

2 protocols using nadph

Indole Biocatalysis Using GroEL/ES

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Chemicals, plasmid construction, and bacterial strains Indole was purchased from Aladdin Chemistry (Shanghai, China). NADPH was purchased from Yeasen Co., Ltd (Shanghai, China), and dimethyl sulfoxide (DMSO) from Genebase Gene-Tech Co., Ltd (Shanghai, China). Recombinant plasmid encoding encoding chaperone GroEL/ES genes was prepared as described previously (Peng et al. 2017 ). E. coli DH5α and BL21(DE3) strains were used for plasmid preparation and protein expression, respectively.

Peng S., Chu Z., Lu J., Li D., Wang Y., Yang S, & Zhang Y. (2023). Overexpression of chaperones GroEL/ES from Escherichia coli enhances indigo biotransformation production of cytochrome P450 BM3 mutant. Biotechnology letters, 45(8).

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Recombinant Protein Expression in E. coli

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All chemicals and solvents were of analytical grade and purchased from commercial sources. NADH, NADPH, NAD⁺ and NADP⁺ were purchased from Yeasen Biotechnology (Shanghai, China). Regents for PCR, FastPfu DNA polymerase, Genomic DNA extraction kit, and Plasmid MiniPrep kit were purchased from Transgen (Beijing, China). The vector pET28a(+), pACYC184 and pETDuet-1 were purchased from Novagen (San Diego, CA, USA). Oligonucleotide primers were synthesized by Personal Biotechnology (Shanghai, China). DNA sequencing was performed by Tsingke Biological Technology (Beijing, China). E. coli BL21(DE3) was purchased from Transgen (Beijing, China).

Zhang B., Sun J., Zheng Y., Mao X., Lin J, & Wei D. (2022). Identification of a novel ene reductase from Pichia angusta with potential application in (R)-levodione production. RSC Advances, 12(22), 13924-13931.

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