After release from 6-well plates, the cells were washed twice with PBS. The cells were collected by centrifugation at 1,500 rpm for 5 min and then stained with 5 μL of 7AAD and PE-labeled Annexin V (BD Biosciences) in 500 μL of 1 × Binding Buffer. The cell apoptosis rate was determined by a flow cytometry within 1 h.
7aad and pe labeled annexin 5
Manufactured by BD
7AAD and PE-labeled Annexin V are fluorescent dyes used in flow cytometry applications to detect and quantify apoptosis. 7AAD is a DNA-binding dye that can be used to identify late apoptotic or necrotic cells. PE-labeled Annexin V binds to phosphatidylserine, which is exposed on the surface of cells undergoing apoptosis.
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Lab products found in correlation
2 protocols using 7aad and pe labeled annexin 5
1
Apoptosis Analysis by Flow Cytometry
2
Quantification of Cell Apoptosis
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Cells were collected 48 h after transfection and washed twice with cold phosphate-buffered saline (PBS). For the ABHD11-AS1 plasmid transfection, cell apoptosis was quantified using 7AAD and PE-labeled Annexin V (BD Biosciences) with flow cytometry, according to the manufacturer’s protocol. For siRNA transfection, cell apoptosis was quantified using annexin V–FITC and PI (BD Biosciences) with flow cytometry. The apoptosis rate was determined by flow cytometry within 1 h.
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