Np19 bsa

Manufactured by Biosearch Technologies

NP19-BSA is a proprietary labeling reagent developed by Biosearch Technologies. It is designed for the covalent attachment of biomolecules, such as proteins, peptides, or oligonucleotides, to nanoparticle surfaces. The core function of NP19-BSA is to facilitate the conjugation of these biomolecules to nanoparticles, enabling the creation of functionalized nanoparticle-based platforms for various applications.

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Lab products found in correlation

Vector blue alkaline phosphatase substrate kit 3, by Vector Laboratories (1 mentions) Alkaline phosphatase conjugated to streptavidin, by Vector Laboratories (1 mentions) Multiscreen 96 well filtration plate, by Merck Group (1 mentions) Np20 bsa, by Biosearch Technologies (1 mentions)

2 protocols using np19 bsa

Quantifying Antigen-Specific Antibody Responses

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Multi screen 96-well filtration plates (Millipore, Billerica, MA) were coated with 4-hydroxy-3-nitrophenyl acetyl conjugated to bovine serum albumin (NP19-BSA, Biosearch Technologies, Petaluma, CA) at a concentration of 10 μg/ml overnight at 4°C. Splenocyte suspensions from immunized mice were initially plated at 1 × 10⁶ cells per well and then diluted serially (1:2) in RPMI1640 medium containing 10% FBS and incubated for six hours at 37°C. Bound antibodies were detected using biotinylated anti-mouse IgM and IgG Abs (Jackson Immunoresearch Laboratories, West, Grove, PA) followed by streptavidin conjugated to alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed using the Vector Blue Alkaline–Phosphatase Substrate kit III (Vector Laboratories, Burlingame, CA). ELISpots were counted using a computerized imaging video system (Cellular Technology, Shaker Heights, OH).

Vuyyuru R., Shen S, & Manser T. (2015). Testing the role of the FcγRIIB immunoreceptor tyrosine-based inhibitory motif in regulation of the B cell immune response. Immunity, Inflammation and Disease, 3(3), 247-264.

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Affinity Maturation Assessment via NP-ELISA

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To examine the affinity maturation, NP specific ELISAs were performed using plates coated with either NP_1-9-BSA or NP_>20-BSA (Biosearch Technologies) diluted in PBS at 5μg/ml. The ratio of high-affinity (NP_1-9-BSA) to low-affinity (NP_>20-BSA) for a given sample was determined as the OD titer of serum antibodies bound to NP_1-9-BSA, divided by the OD titer of antibodies bound to NP_>20-BSA.

Shinde R., Shimoda M., Chaudhary K., Liu H., Mohamed E., Bradley J., Kandala S., Li X., Liu K, & McGaha T.L. (2015). B cell-intrinsic Indoleamine 2,3-dioxygenase1 regulates humoral immunity to T cell independent antigens. Journal of immunology (Baltimore, Md. : 1950), 195(5), 2374-2382.

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