Elx808 microplate spectrophotometer

Manufactured by Agilent Technologies

Sourced in United States

The ELX808 microplate spectrophotometer is a compact and versatile laboratory instrument designed for absorbance-based measurements. It provides accurate and reliable data for a range of applications, including clinical diagnostics, drug discovery, and environmental testing. The ELX808 features multiple wavelength capabilities, automated plate handling, and intuitive software for streamlined data analysis.

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Lab products found in correlation

Cell counting kit 8 cck 8, by Beyotime (1 mentions) Bi2536, by Beyotime (1 mentions) Facscalibur flow cytometry, by BD (1 mentions) Xevo g2 xs qtof mass spectrometer, by Waters Corporation (1 mentions) Inertsustain c18 column, by Shimadzu (1 mentions) Bx51 fluorescence microscopy, by Olympus (1 mentions) Lc 20at hplc system, by Shimadzu (1 mentions) Ascend 600 mhz instrument, by Bruker (1 mentions)

Spelling variants of this product

ELx808 (759 citations) Microplate spectrophotometer (506 citations) ELx808 spectrophotometer (8 citations) ELx808 microplate spectrophotometer reader (2 citations) Elx808 Absorbance Microplate spectrophotometer (2 citations)

4 protocols using elx808 microplate spectrophotometer

Cell Viability Assay with CCK-8

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Cells were cultured in complete medium containing dimethyl sulfoxide (DMSO, as control), ICT (Winherb Medical Science), or BI 2536 (Beyotime) at the indicated concentrations for the indicated time. Cell viability was measured by Cell Counting Kit-8 (CCK-8, Beyotime) following the manufacturer’s protocol. The absorbance at 450 nm was measured using an ELX808 microplate spectrophotometer (BioTek Instruments). The cell viability ratio was calculated as:

Cell viability rate = (average OD of ICT or / and BI 2536 treated wells - average OD of blank wells) / (average OD of control wells - average OD of blank wells) \times 100 %

Each experiment was carried out in six replicates and results were calculated over three independent experiments.

Zhang C., Xu H., Sui X., Chen L., Chen B., Lv H., Wang S, & Wang X. (2022). Icaritin inhibits PLK1 to activate DNA damage response in NK/T cell lymphoma and increases sensitivity to GELOX regime. Molecular Therapy Oncolytics, 25, 288-304.

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Multi-Instrumental Analytical and Biological Assays

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The HRESIMS analyses were performed on a Waters Xevo G2-XS QTof mass spectrometer (Waters Corp., Milford Massachusetts, America). NMR spectra were recorded on an Ascend 600 MHz instrument (Bruker-Biospin, Billerica, MA, America). The analytical experiments were performed on a Shimadzu LC-20AT HPLC system (Shimadzu Corp., Kyoto, Japan) equipped with a Shimadzu InertSustain C18 column (4.6 I.D. × 250 mm, 5 μm, S/N 6LR98081). A Hanbon NP700 semipreparative HPLC (Hanbon Sci. & Tech., Jiangsu, China) equipped with a Shimadzu InertSustain C18 column (10 I.D. × 250 mm, 5 μm, S/N 7ER43006) was used for purifying compounds. Biological assays were monitored on a BioTek ELx808 microplate spectrophotometer (BioTek Instruments, Inc., Winooski, America), a BD FACSCalibur flow cytometry (BD Biosciences, Franklin Lakes, America) and an Olympus BX-51 Fluorescence Microscopy (Olympus Corporation, Tokyo, Japan).

Xu K., Guo C., Meng J., Tian H., Guo S, & Shi D. (2019). Discovery of Natural Dimeric Naphthopyrones as Potential Cytotoxic Agents through ROS-Mediated Apoptotic Pathway. Marine Drugs, 17(4), 207.

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Cell Viability and Clonogenic Assays

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Cells were seeded in triplicate in 96-well plates at a density of 2000 cells per well. Cell viability was assessed with a Cell Counting kit-8 (CCK-8, Dojindo, Japan) according to the manufacturer’s protocol. The absorbance at 450 nm was measured using an ELX808 microplate spectrophotometer (BioTek Instruments, Winooski, VT, USA). To assess the clonogenic capacity of cells in vitro, 500 cells were plated in six-well culture plates in triplicated. The cells were allowed to grow for 10–14 days to form colonies, and then fixed with methanol and stained with crystal violet. The number of colonies was counted.

Cai H.Q., Zhang M.J., Cheng Z.J., Yu J., Yuan Q., Zhang J., Cai Y., Yang L.Y., Zhang Y., Hao J.J., Wang M.R, & Wan J.H. (2021). FKBP10 promotes proliferation of glioma cells via activating AKT-CREB-PCNA axis. Journal of Biomedical Science, 28, 13.

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Quantifying BDNF Levels in Cell Media

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The BDNF content was measured in the medium using the BDNF Human BDNF ELISA Kit, according to the manufacturer's protocol. Briefly, 100–100 µL of the medium was added to wells and incubated overnight at 4 °C. Next, the wells were washed, and a biotinylated anti-BDNF antibody was added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin was pipetted into wells. Then, the wells were washed, TMB (3,3′,5,5′-tetramethylbenzidine) substrate solution was added to wells, and the color developed in proportion to the amount of bound BDNF. After adding the stop solution, the color intensity was measured at 450 nm using an ELx808 microplate spectrophotometer (BioTek). Protein concentrations in each sample were measured using a BCA™ Protein Assay Kit and values of BDNF were corrected for the total amount of protein in the cell lysate of the samples.

Tevzadze G., Barbakadze T., Kvergelidze E., Zhuravliova E., Shanshiashvili L, & Mikeladze D. (2021). Gut neurotoxin p-cresol induces brain-derived neurotrophic factor secretion and increases the expression of neurofilament subunits in PC-12 cells. AIMS Neuroscience, 9(1), 12-23.

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