N2a cells were seeded on cover slips in a 24-well plate. After the experimental treatments, the One Step TdT-mediated dUTP nick-end labeling (TUNEL) Apoptosis Assay Kit (Beyotime) was used. This procedure was performed away from light. The samples were prepared as described for immunofluorescence microscopy until antibody incubation. Then, the samples were incubated with TUNEL stain reagent for 1 hour at 37°C. 4′,6-Diamidino-2-phenylindole stain reagent (Beyotime) was used to visualize the nucleus for 10 minutes at 37°C. All slides were photographed using a Nikon confocal imaging system. Images were analyzed using ImageJ software version 1.52a.
One step tdt mediated dutp nick end labeling tunel apoptosis assay kit
Manufactured by Beyotime
The One Step TdT-mediated dUTP nick-end labeling (TUNEL) Apoptosis Assay Kit is a tool for detecting and quantifying apoptosis, a type of programmed cell death. The kit utilizes the TUNEL method to label DNA strand breaks, a hallmark of apoptosis. It provides a simple, reliable, and sensitive approach to analyze apoptosis in various cell types and tissues.
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2 protocols using one step tdt mediated dutp nick end labeling tunel apoptosis assay kit
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Quantifying Apoptosis in N2a Cells
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TUNEL Apoptosis Assay Protocol
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Apoptosis was analyzed using One Step TdT-mediated dUTP Nick-End Labeling (TUNEL) Apoptosis Assay Kit (Beyotime) according to the manufacturer's suggestions. Sections were depara nized and treated with 20µg/ml proteinase K for 30min at 37°C, and then washed for three times. Subsequently, these sections were darkly incubated with TUNEL reaction for 1h at 37°C. At last, uorescence-labeled images were visualized under a uorescence microscopy.
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