Anti erk1 2

Spautin-1(S7888), 3-methyladanine (3-MA, S2767), Z-VAD-FMK (S7023), SP600125 (S1460), SB230580 (S1076), LY3214996 (S8534), Gefitinib (S1025) and Enzalutamide (MDV3100, S1250) were purchased from Sellectchem (Houston, TX, USA). SKP2-C25 (M60136) was obtained from Xcessbio Biosciences, Inc. (San Diego, CA). USP10 (sc-76,811), USP13 (sc-76,815) and Glut1 (sc-35,493) siRNAs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies: anti-Glut1 (ab652), anti-USP13 (ab109264) (Abcam, Cambridge, MA); anti-GAPDH (BS60630), anti-Ki67 (BS1454) (Bioworld Technology, Inc., Louis Park, MN, USA); anti-USP10(#8501), anti-SKP2(#2652), anti-p27(#3686), anti-CDK4(#12790), anti-CDK2(#2546), anti-CyclinD1(#2978), anti-p15(#4822), anti-p21(#2947), anti-PARP(#9532), anti-Bim(#2933), anti-Bax(#14796), anti-Bcl-2(#15071), anti-activated Caspase-3(#9664), anti-phospho-JNK (#9255), anti-JNK(#9252), anti-phospho-ERK1/2(#4370), anti-ERK1/2(#4695), anti-phospho-p38(#4511), anti-p38(#8690), anti-phospho-MKK4(#4514), anti-MKK4(#9152), anti-phospho-MEK1/2 (#9154), anti-MEK1/2(#4694), anti-phospho-EGFR (Tyr1173)(#4407), anti-phospho-EGFR (Tyr1068)(#3777), anti-EGFR(#2085) (Cell Signaling Technology, Beverly, MA, USA).

Escherichia coli 0127:B8 was obtained from ATCC (12740). LPS (Escherichia coli 0127:B8) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rolipram was obtained from Enzo Life Science (Farmingdale, NY, USA). Rabbit anti-p38, anti-phospho-p38, anti-ERK1/2, anti-phospho-ERK1/2, anti-JNK, anti-phospho-JNK, and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG were procured from Bioworld Technology (St. Louis, MN, USA). Rabbit anti-NFκB p65 and anti-phospho-NFκB p65 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Alexa Fluor® 488 goat anti-rabbit IgG (H + L) was purchased from Molecular Probes (Eugene, OR, USA). MILLIPLEX® MAP Mouse Cytokine/Chemokine Magnetic Bead Panel Kit for 96-Well Plate Assay was purchased from EMD Millipore (Darmstadt, Germany).

Recombinant human IL-6 was from Pepro Tech. LC3B; 3-MA and baf A1 were from Sigma Aldrich. Anti-ERK1/2, anti-phospho-ERK1/2, anti-Akt, and anti-phospho-Akt antibodies were from Bioworld Technology. Anti-STAT3, anti-phospho-mTOR, anti-mTOR, anti-p62, anti-Bcl-2, anti-Beclin 1, and anti-VPS34 antibodies were from Cell Signaling Technology (CST). Anti-phospho-STAT3 was from Wuhan Boster Biological Technology, Ltd. The STAT3 inhibitor LLL12 was from Merck Millipore. pcDNA3.1(+) (V790-20) was from Invitrogen.