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139in dota maleimide

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139In-DOTA-maleimide is a radiochemical product used in the labeling of biomolecules. It is composed of the radioisotope Indium-139 chelated to the DOTA chelating agent, which is functionalized with a maleimide group. This product is intended for use in research and development applications.

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Lab products found in correlation

Foxp3 fix perm buffer, by Thermo Fisher Scientific (2 mentions) Ir dna intercalator, by Standard BioTools (2 mentions) Eq calibration beads, by Standard BioTools (2 mentions) Cytof2, by Standard BioTools (2 mentions)

2 protocols using 139in dota maleimide

1

Mass Cytometric Analysis of PBMCs and HLAC

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Mass cytometric analysis was performed as previously described (59 (link)). Cells were isolated from fresh PBMCs or HLAC as described above and stained with a panel of lanthanide metal-conjugated antibodies (Table S1). Antibody staining was performed in a volume of 100 μL for 45 min, which was treated with 139In-DOTA-maleimide (Macrocyclics) to label dead cells and fixed overnight with PBS plus 2% PFA. Cells were permeabilized with FoxP3 Fix/Perm buffer (Thermo Fisher) per the manufacturer’s protocol and then labeled with 1:4,000 191/193Ir DNA intercalator (Fluidigm). Cells were washed, resuspended in distilled water, and analyzed on a CyTOF2 (Fluidigm) at the UCSF Parnassus Flow Cytometry Core. EQ calibration beads (Fluidigm) were included and used for normalization across runs.
Packard T.A., Schwarzer R., Herzig E., Rao D., Luo X., Egedal J.H., Hsiao F., Widera M., Hultquist J.F., Grimmett Z.W., Messer R.J., Krogan N.J., Deeks S.G., Roan N.R., Dittmer U., Hasenkrug K.J, & Greene W.C. (2022). CCL2: a Chemokine Potentially Promoting Early Seeding of the Latent HIV Reservoir. mBio, 13(5), e01891-22.
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2

Mass Cytometry Analysis of PBMCs

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Mass cytometric analyses was performed as previously described 68 (link) . Cells were isolated from fresh PBMCs or HLAC as described above and stained with a panel of lanthanide metalconjugated antibodies (See Table S1). Antibody staining was performed in a volume of 100 µL for 45 min, treated with 139In-DOTA-maleimide (Macrocyclics) to label dead cells, and fixed overnight with PBS + 2% PFA. Cells were permeabilized with FoxP3 Fix/perm buffer (ThermoFisher) per the manufacturer's protocol, then labelled with 1:4,000 191/193Ir DNA intercalator (Fluidigm). Cells were washed, resuspended in distilled water, and analyzed on a CyTOF2 (Fluidigm) at the UCSF Parnassus Flow Cytometry Core. EQ calibration beads (Fluidigm) were included and used for normalization across runs.
Packard T., Korte T., Herzig E., Rao D., Luo X., Egedal J.H., Hsiao F., Widera M., Hultquist J.F., Grimmett Z.W., Messer R.J., Krogan N.J., Deeks S.G., Roan N.R., Dittmer U., Hasenkrug K.J., & Greene W.C. (2021). The CCL2 Chemokine Promotes Early Seeding of the Latent HIV Reservoir.
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