Type i4000

Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (HBsAb), HBeAg, hepatitis B e antibody (HBeAb), hepatitis B core antibody (HBcAb), HBV DNA and alpha-fetoprotein (AFP) were quantified as described in a previous study[24 (link)]. Briefly, ALT and AST were determined using an automated biochemical system (Siemens Healthcare Diagnostics, USA). Serum HBsAg, HBsAb, HBeAg, HBeAb and HBcAb were measured using the automatic immune fluorescence analyser Abbott Type i4000 (Abbott Laboratories, USA), following the manufacturer’s instructions. Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) in a Roche LightCycler 480 Real-Time PCR System (Roche, Switzerland) with a low quantification limit of 500 IU/ml.

Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by automated biochemical technique (Siemens Healthcare Diagnostics, USA) with cutoff value of 40 IU/L. Serum HBsAg, anti-HBs, hepatitis B e antigen (HBeAg), anti-HBe and anti-HBc were measured on the wholly automatic immune fluorescence analyzer Abbott Type i4000 (Abbott Laboratories, USA) using the original, corollary commercial kits. Serum HBV DNA was quantified using the TaqMan polymerase chain reaction (PCR) assay (Sansure Biotech, China) on Roche LightCycler 480 Real-Time PCR System (Roche, Switzerland) with a low limit of quantification of 500 IU/ml.

Liver function tests and serum HBV markers were conventionally performed in the clinical laboratory of the First Affiliated Hospital of Fujian Medical University. Serum hepatitis B s antigen (HBsAg), anti-HBs, hepatitis B e antigen (HBeAg), anti-HBe, and anti-HBc were determined on the wholly automatic immune fluorescence analyzer Abbott Type i4000 (Abbott Laboratories, USA) using the original attached commercial kits. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were assayed using an automated biochemical technique (Siemens Healthcare Diagnostics, USA). Serum HBV DNA was quantified using the TaqMan PCR assay (Sansure Biotech, China) on an ABI 7500 real-time PCR system (Life Technologies, USA) with a lowest detection limit of 20 IU/ml.