Kg 501

Manufactured by Selleck Chemicals

Sourced in United States

The KG-501 is a laboratory-grade mixer designed for efficient and controlled blending of various liquids and suspensions. It features a variable-speed motor and a sturdy construction for reliable performance in the laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Bt549, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Skbr3, by American Type Culture Collection (1 mentions) Hs578t, by American Type Culture Collection (1 mentions) Pmirglo e1330, by Promega (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Nacalai Tesque (1 mentions) Multiskan skyhigh microplate reader, by Thermo Fisher Scientific (1 mentions) Propranolol, by Selleck Chemicals (1 mentions) Anlotinib, by Selleck Chemicals (1 mentions) Apatinib, by Selleck Chemicals (1 mentions) Mg132, by Selleck Chemicals (1 mentions) Individual bas, by Merck Group (1 mentions) Sq22536, by Selleck Chemicals (1 mentions) Sb239063, by Selleck Chemicals (1 mentions)

6 protocols using kg 501

Breast Cancer Cells Characterization and Culture

Cited 1 time

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Human breast cancer cells (MCF-7, SKBr-3, T47D, MDA-MB-468, MDA-MB-231, BT-549, Hs578T) were purchased from the American type culture collection (ATCC). The MCF-7/Twist and MCF-7/vector cells were constructed as described previously [16 (link)]. All BC cells were cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA) containing 10% FBS(Gibco). Cells were maintained in a humidified incubator at 37 °C with 5% CO₂.
KG-501 was purchased from Selleck (Shanghai, China), and dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Puromycin and G418 were purchased from Thermo Fisher Scientific (USA).

Qiao Y., Jin T., Guan S., Cheng S., Wen S., Zeng H., Zhao M., Yang L., Wan X., Qiu Y., Li Q., Liu M, & Hou Y. (2021). Long non-coding RNA Lnc-408 promotes invasion and metastasis of breast cancer cell by regulating LIMK1. Oncogene, 40(24), 4198-4213.

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Regulation of FAIM Promoter by CREB

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The mouse FAIM proximal promoter genomic region (NC_000075.7:98866745-98868446 as described in the National Center for Biotechnology Information database, −1681 to +20, −520 to +20 and the CRE element mutant promoter-reporter constructs −1681 to +20) were inserted upstream of a luciferase reporter gene with a flanking sequence into the BglII and apaI-digested pmirGLO (E1330, Promega) vector to generate pmirGLO-FAIM (WT, mutant, truncated), respectively. The coding region of the mouse Creb1 gene (Mus musculus, transcript variant C, NM_001037726.1) was cloned into the pcDNA3.1(+) vector (pcDNA3.1(+)-CREB). The plasmids were constructed and sequenced by Genewiz (Suzhou, CHINA). AML12 were transfected with FAIM promoter-luciferase reporter plasmids and pcDNA3.1(+)-CREB vector using Lipofectamine 3000 according to the manufacturer’s instructions. In CREB inhibitor experiment, AML12 were transfected with FAIM reporter plasmids for 24 h and treated with 1 μM PACAP for an additional 24 h in the presence or absence of the CREB inhibitor KG-501 (S8409, 18228-17-6, Selleck Chemicals, USA). Cells then lysed in 100 μl lysis buffer and detected using the Dual-Luciferase Reporter Assay System (E1910, Promega). Renilla luciferase activity was used as a control.

Luo W., Dai J., Liu J., Huang Y., Zheng Z., Xu P, & Ma Y. (2022). PACAP attenuates hepatic lipid accumulation through the FAIM/AMPK/IRβ axis during overnutrition. Molecular Metabolism, 65, 101584.

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Cytotoxicity Evaluation of CREB Inhibitors

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MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide) assay kit (Nacalai Tesque, Japan) was used according to the manufacturer’s instructions. 1×10³ HEC50B cells in DMEM containing FBS were seeded to each well of 96 well plate. After 24 hours, the medium was replaced to DMEM containing FBS supplemented with CREB inhibitor, KG501 (Selleck, USA) or compoud3i (Selleck, USA), at concentrations of 0.5, 1, 5 and 10 μM. As vehicle control, DMSO was supplemented with DMEM. After 24, 48, 72 and 96 hours, HEC50B cells were incubated in medium with 0.5 mg/mL MTT solution for 2 hours and lysed with 0.04 M HCl in isopropyl alcohol. Absorbance at 570 nm was measured using Multiskan SkyHigh Microplate Reader (Thermo Fisher Scientific).

Kato H., Saeki N., Imai M., Onji H., Yano A., Yoshida S., Sakaue T., Fujioka T., Sugiyama T, & Imai Y. (2023). LIM1 contributes to the malignant potential of endometrial cancer. Frontiers in Oncology, 13, 1082441.

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NSCLC Cell Line Culture and Drug Preparation

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Human NSCLC cell lines H522, H460, A549, and H292 were purchased from the American Type Culture Collection (ATCC). All cell lines were cultured in RPMI 1640 medium (Gibco) containing 10% fetal bovine serum (Gibco) at 37 °C in a 5% CO₂ incubator. Apatinib (Selleckchem) and anlotinib (Selleckchem) were dissolved in dimethylsulfoxide (DMSO) and stored at −80 °C (20 mM, stock solution). Propranolol (Selleckchem) was dissolved in DMSO and stored at −80 °C (50 mM, stock solution). KG501 (Selleckchem) was dissolved in DMSO and stored at −80 °C (25 mM, stock solution).

Xu Y., Wang J., Wang X., Zhou X., Tang J., Jie X., Yang X., Rao X., Xu Y., Xing B., Li Z, & Wu G. (2022). Targeting ADRB2 enhances sensitivity of non-small cell lung cancer to VEGFR2 tyrosine kinase inhibitors. Cell Death Discovery, 8, 36.

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Investigating Glioma Cell Line Responses

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The human glioma cell lines GOS-3, T98G, U118MG, LN229, U251MG, SF268, U343MG, U373MG, HS683 and TJ905 were purchased from the Cobioer Biotechnology Company (Nanjing, China) and National Infrastructure of Cell Line Resource (Beijing, China). All cells were cultured in high-glucose DMEM except for SF268 in 1640 and T98G in MEM with containing 10% fetal bovine serum, 100 mg/mL streptomycin, and 100 U/mL penicillin and 73 maintained in a humidified atmosphere containing 5% CO2 at 37 °C. The cell lines were incubated with the p-Akt activator SC79, p-CREB inhibitor KG-501 and MG132 (Selleck) for different lengths of time.

Cai H.Q., Zhang M.J., Cheng Z.J., Yu J., Yuan Q., Zhang J., Cai Y., Yang L.Y., Zhang Y., Hao J.J., Wang M.R, & Wan J.H. (2021). FKBP10 promotes proliferation of glioma cells via activating AKT-CREB-PCNA axis. Journal of Biomedical Science, 28, 13.

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Bile Acid Signaling Pathway Regulation

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Individual BAs were obtained from Sigma-Aldrich (St. Louis, MO, USA), Steraloids Inc. (Newport, RI, USA) and TRC Chemicals (Toronto, ON, Canada). Cells were treated with Individual BAs at 25, 50, 75, and 100 µM for 24 hours. A combination of equal amounts of TCA and GCA (50 µM each, termed as 12α-OH BA1), or a combination of equal amount of TDCA and GDCA (50 µM each, termed as 12α-OH BA2) for varied lengths of time as specified in the Results section. Cells treated with DMSO were used as a vehicle control. SCH772984 (S7101, ERK1/2 inhibitor), SP600125 (S1460, JNK inhibitor),SB239063 (S7741, p38MAPK inhibitor), SQ22536 (s8283, inhibitor for adenylyl cyclase (AC)) and KG-501 (s8409, inhibitor for CREB) were purchased from Selleck Chemicals. TGR5 antagonist 5β-cholanic acid (C7628) was purchased from Sigma-Aldrich. The siRNA targeting human TGR5 (sc-61678-SH) and control shRNA plasmid (sc-108060) were purchased from Stanta Cruz (Dallas, TX).

Xie G., Jiang R., Wang X., Liu P., Zhao A., Wu Y., Huang F., Liu Z., Rajani C., Zheng X., Qiu J., Zhang X., Zhao S., Bian H., Gao X., Sun B, & Jia W. (2021). Conjugated secondary 12α-hydroxylated bile acids promote liver fibrogenesis. EBioMedicine, 66, 103290.

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