HiPCo SWCNTs were purchased from NanoIntegris (batch #27-104). Serotonin hydrochloride (hydroxytryptamine hydrochloride), dopamine hydrochloride, acetylcholine, γ-aminobutyric acid (GABA), tyrosine, glutamate, octopamine, HTP, HIAA, MTP, fluoxetine, 3,4-MDMA, 25-NMOMe, and quetiapine were purchased from Sigma-Aldrich. All ssDNA sequences were purchased from Integrated DNA Technologies (IDT; USA).
γ aminobutyric acid gaba
Manufactured by Merck Group
Sourced in United States, United Kingdom, Germany
γ-aminobutyric acid (GABA) is a chemical compound that functions as a neurotransmitter in the central nervous system. It is a key inhibitory neurotransmitter that plays a crucial role in regulating neuronal excitability and maintaining a balance between excitation and inhibition in the brain.
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Lab products found in correlation
Dopamine hydrochloride, by Merck Group (2 mentions)
Acetylcholine, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Glutamate, by Merck Group (1 mentions)
Tyrosine, by Merck Group (1 mentions)
Octopamine, by Merck Group (1 mentions)
Quetiapine, by Merck Group (1 mentions)
Fluoxetine, by Merck Group (1 mentions)
Dopamine, by Merck Group (1 mentions)
Serotonin, by Merck Group (1 mentions)
L glutamic acid monosodium salt hydrate, by Merck Group (1 mentions)
Acetylcholinesterase from electrophorus electricus ache, by Merck Group (1 mentions)
D glucose monohydrate, by Merck Group (1 mentions)
Picrotoxin, by Bio-Techne (1 mentions)
2 3 dioxo 6 nitro 1 2 3 4 tetrahydrobenzo f quinoxaline 7 sulfonamide disodium salt nbqx, by Bio-Techne (1 mentions)
Pilocarpine hydrochloride, by Merck Group (1 mentions)
Sodium nitroprusside snp, by Merck Group (1 mentions)
27 protocols using γ aminobutyric acid gaba
1
Neurochemical Interaction Assay
2
Potentiometric Neurotransmitter Sensor
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Sodium tetrakis[3,5-bis(trifluoromethyl)phenyl]borate (NaTFPB), calix[4]arene (CX4), 2-nitrophenyl octyl ether (o-NPOE), high-molecular-weight poly(vinyl chloride) (PVC), tetrahydrofuran (THF, inhibitor-free, for HPLC), potassium hydrogen phosphate, potassium dihydrogen phosphate, D-(+)-Glucose monohydrate, potassium chloride, sodium chloride, calcium chloride, magnesium chloride, ammonium chloride, hydrochloric acid (HCl), -Aminobutyric acid (GABA), L-Glutamic acid monosodium salt hydrate, serotonin, dopamine, Acetylcholinesterase from Electrophorus electricus (AChE), and OmniPur Tris HCl buffer were purchased from Sigma Aldrich. We prepared all stock solutions with MiliQ deionized water (resistivity of 18.2 MΩ·cm) unless stated otherwise. The sheep brain tissue was purchased from a local market (Tehran Market, Los Angeles, CA, USA).
3
Pharmacological Evaluation of Neuroreceptors
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Pilocarpine hydrochloride and γ-aminobutyric acid (GABA) was purchased from Sigma-Aldrich (Dorset, UK) and dissolved in saline on the day of experiment. 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX, 10 μM), DL-2-Amino-5-phosphonopentanoic (DL-AP5; 50 μM), picrotoxin (100 μM) and (2 S)-3-[[(1 S)-1-(3,4-Dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl) phosphinic acid (CGP55845; 1 μM), used in electrophysiological recordings in vitro to block AMPA, NMDA-, GABAA- and GABAB-receptors respectively, were all from Tocris Bioscience (Bristol, UK).
4
Shoot Tip Explant Experiments with SNP and GABA
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After data from the carbohydrate study were analyzed, all culture lines were transferred to version 4 medium (Figure 2 ) for at least a month. The number of additional lines rose to sixteen, including the nine used in the antioxidant study. Of those, six had sufficient numbers of shoots to be used in only one of two concurrent experiments. In the first, six shoot tip explants from the eleven core lines and thirteen additional lines were placed on version 4 medium with 0, 1.3, 2.6, 5.2, or 10.5 mg L−1 sodium nitroprusside (SNP) (Sigma) for a total of 720 shoots. In the second experiment, six shoot tip explants from the eleven core lines and thirteen additional lines were placed on version 4 medium with 0, 51.5, or 103 mg L−1 γ-aminobutyric acid (GABA) (Sigma) for a total of 432 shoot tips. Necrosis and total shoots with lengths greater than 0.25 cm were counted after 80 days.
5
RBBP6 Regulation in Cervical Cancer
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Human cervical cancer tissue sections were purchased from Cybrdi (Rockville, MD, USA) after obtaining ethical clearance issued by the University of the Witwatersrand ethics committee (number M140801), and human cell lines SiHa, MRC-5, and HeLa were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). SiHa and HeLa cells were derived from the most prevalent squamous cervical carcinomas and the less prevalent adenocarcinomas, respectively, and both cell lines expressed wild-type p53. Silencing of RBBP6 was achieved using Ambion’s Silencer select Pre-designed siRNA supplied by Thermo Fisher Scientific (Waltham, MA, USA). RBBP6 overexpression was achieved using the pCMV6-AC-GFP mammalian expression vector supplied by Blue Heron Biotech, LLC (Bothell, WA, USA) to deliver the open reading frame of RBBP6 transcript variant 3 (NM_032626.5) into the cell lines. Camptothecin (Calbiochem, San Diego, CA, USA) and γ-aminobutyric acid (GABA) (Sigma-Aldrich Co., St Louis, MO, USA) were used as anticancer agents.
6
GABA Treatment of Maize Cultivar
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Maize seeds, “Zhengdan 958” (Zea mays L.), supplied by the Henan Academy of Agricultural Sciences, were used in this experiment. γ-Aminobutyric acid (GABA) (99% purity, CAS No. 56-12-2) was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
7
GABA Estimation in Brain Tissue
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Bicuculline, clonazepam, diazepam, flumazenil, methyl-β-carboline-3-carboxylate (FG7142), pentylenetetrazole, sodium valproate, reduced glutathione, thiobarbituric acid, n-butanol, pyridine, sodium dodecyl sulphate, 5′5-dithiobis (2-nitrobenzoic acid) and trichloroacetic acid were obtained from Sigma, St. Louis, MO, USA. All other chemicals and reagents used in the brain γ-aminobutyric acid (GABA) content estimation were obtained from Sigma, St. Louis, MO, USA. diazepam was obtained from Roche (France).
8
Phytochemical Analysis and Antioxidant Assay
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Methanol and ethanol of HPLC grade, Folin-Ciocalteu reagent, gallic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,6-dimethoxy-1,4-benzoquinone (DMBQ), and γ-aminobutyric acid (GABA), were purchased from Sigma Chemical Co. (St. Louis, MO, USA). All other chemicals and reagents were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
9
Tracking Cell Proliferation Using GABA
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ALDH1A3 OE and vector control MDA-MB-231 cells were seeded in 6-well plates and incubated overnight at 37 °C to promote cell adhesion. Adherent MDA-MB-231 cells were stained with 1.25 μM Oregon Green 488 dye (ThermoFisher Scientific) in warm serum-free DMEM for 45 min at 37 °C. To obtain non-proliferative control cells, cells were harvested and fixed in 1% paraformaldehyde. These cells give the highest fluorescence at t = 0 h. The rest of the Oregon Green-stained cells were treated with 50 µM γ-aminobutyric acid (GABA, Sigma-Aldrich) and incubated at 37 °C for 24, 48 and 72 h. At the end of the incubations, the cells were harvested, and the Oregon Green fluorescence was measured using a Celesta instrument (BD Bioscience, Mississauga, ON). The number of cell divisions (n) that took place was calculated using the formula MCFcontrol = 2n × MCFtreatment. MCF denotes the mean channel fluorescence.
10
Radioligand Binding Assay Protocol
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