The total protein content of the conditioned medium obtained from MSCs in 2D and 3D cultures was measured by the bicinchoninic acid (BCA) method, a colorimetric method. The procedure was performed with the directions in the commercially purchased protein quantification kit (KTD3001, Abbkine Co., USA), the absorbances were measured with a microplate reader (Allsheng AMR-100 Microplate Reader AS-16050-00), and the protein amount was calculated as μg/ml. For the cytokine analysis in the conditioned medium, commercially purchased “enzyme-linked immunosorbent assay” (ELISA) kits were used, and the levels of IFN-γ (EK0373, Boster, Wuhan, China), IL 17 (EK0430, Boster, Wuhan, China), IL-4 (EK0404, Boster, Wuhan, China), and IL-10 (EK0416, Boster, Wuhan, China) were detected. The manufacturer’s instructions were followed during the analyses, and the measurements were made with a 450-nm microplate reader (Allsheng AMR-100 Microplate Reader AS-16050-00). Each sample was performed in duplicates to reduce the error. The concentrations of the samples were calculated as pg/ml.
Amr 100 microplate reader as 16050 00
Manufactured by Allsheng
Sourced in United States, China
The AMR-100 Microplate Reader AS-16050-00 is a laboratory instrument designed for absorbance measurements in microplates. It is capable of reading and analyzing samples in 96-well microplates.
Automatically generated - may contain errors
3 protocols using amr 100 microplate reader as 16050 00
1
Quantification of Secreted Proteins by MSCs
2
Quantification of Urinary Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of urine SDF‐1a and VEGF cytokines were quantified with an calorimetric enzyme‐linked immunosorbent assay (Abbkine, China) and measured with a microplate reader (Allsheng AMR‐100 Microplate Reader AS‐16050‐00, China).
3
Cytokine Profiling in Animal Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cardiac blood was collected from animals at week 7 for cytokine analyses. Serum levels of C-peptide Cloudclone (CEA447Ra, Uscn Life Science Inc. Wuhan, China), insulin (CEA448Ra, Uscn Life Science Inc. Wuhan, China), Th1-related IFN-γ (SEA049Ra, Uscn Life Science Inc. Wuhan, China), Th17-related IL-17 (SEA063Ra, Uscn Life Science Inc. Wuhan, China) as pro-inflammatory, and Th2-related IL-4 (SEA063Ra, Uscn Life Science Inc. Wuhan, China) and Treg-related IL-10 (SEA056Ra, Uscn Life Science Inc. Wuhan, China) cytokines as anti-inflammatory were evaluated by ELISA analysis. The manufacturer’s instructions were followed during the analyses, and the measurements were made with a 450-nm microplate reader (Allsheng AMR-100 Microplate Reader AS-16050-00). With the measured absorbance values, the concentrations of the samples were calculated as pg/ml.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!