B. tequilensis was inoculated into flasks containing sterile LB broth (without NaCl), tryptone (BD Biosciences, Franklin Lakes, NJ, USA), and yeast extract, and the flasks were incubated for 24 hours (37°C, 200 rpm). After incubation, the culture was centrifuged (10,000 rpm, 10 minutes), and the supernatant was used for the synthesis of AgNPs. Three Erlenmeyer flasks, one containing supernatant with AgNO3 at a concentration of 5 mM, the second containing only the supernatant, and the third containing only AgNO3 solution, were incubated at 60°C for 1 hour. We used the sterile LB broth (without NaCl), tryptone (BD Biosciences), and yeast extract containing 5 mM AgNO3 as a control, to establish that the tryptone and yeast extract could not reduce the Ag+ ions to AgNPs. The supernatant was sterilized by 0.22 μm filter (Merck Millipore, Billerica, MA, USA) after centrifugation at 10,000 rpm for 10 minutes. The concentration of AgNPs was determined as described earlier.34 (link) The AgNPs derived from the culture supernatant of bacteria were called B-AgNPs.
Tryptone
Manufactured by BD
Sourced in United States, Japan, Germany, India, Australia
Tryptone is a complex mixture of peptides and amino acids derived from the enzymatic digestion of casein, a protein found in milk. It is a common component used in culture media for the growth of bacteria and other microorganisms in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract, by BD (61 mentions)
Sodium chloride (nacl), by Merck Group (11 mentions)
Peptone, by BD (9 mentions)
Bacto agar, by BD (8 mentions)
Kanamycin, by Merck Group (6 mentions)
Sodium chloride (nacl), by Fujifilm (5 mentions)
Ampicillin, by Merck Group (4 mentions)
Chloramphenicol, by Merck Group (4 mentions)
K2hpo4, by Merck Group (4 mentions)
Soytone, by BD (4 mentions)
Potassium chloride (kcl), by Fujifilm (4 mentions)
Glycine, by Fujifilm (4 mentions)
Cucl2 2h2o, by Fujifilm (4 mentions)
Na2hpo4 12h2o, by Fujifilm (4 mentions)
Kh2po4, by Fujifilm (4 mentions)
Kh2po4, by Merck Group (4 mentions)
Glycerol, by Merck Group (4 mentions)
Bacto yeast extract, by BD (4 mentions)
Whatman, by Cytiva (4 mentions)
Luria bertani broth, by BD (3 mentions)
118 protocols using tryptone
1
Biogenic Silver Nanoparticle Synthesis
2
Effects of d-Allulose on Bacterial Growth
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The effects of d-allulose on bacterial growth and acid production were examined in a medium constituted from 4 mL of tryptone-yeast extract-glucose (TYG) broth, which is made up of 0.5% tryptone (Becton Dickinson), 0.5% yeast extract (Wako Pure Chemical Industries, Tokyo, Japan), 1% sodium succinate, 1% sodium chloride, and 1% glucose. The broth was adjusted to a pH of approximately 6.3. The broth was inoculated with a 1% (vol/vol) bacterial suspension of the test strain and incubated at the appropriate growth temperature for 1 to 2 d under batch conditions.
At the end of the incubation, the molar growth yield on glucose (Y G ) was calculated by dividing the dry weight of cells by the molar mass of consumed glucose, as determined below. To measure dry weight, cells were collected by centrifugation (1,800 × g, 20 min, 4°C), washed once with distilled water, dried at 105°C for 4 h, and weighed.
At the end of the incubation, the molar growth yield on glucose (Y G ) was calculated by dividing the dry weight of cells by the molar mass of consumed glucose, as determined below. To measure dry weight, cells were collected by centrifugation (1,800 × g, 20 min, 4°C), washed once with distilled water, dried at 105°C for 4 h, and weighed.
3
Bacterial Motility Assays: Swimming and Twitching
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Swimming plates contained 1% tryptone (Difco Laboratories, USA), 0.5% NaCl (Merck, Germany), and 0.3% agar (Difco Laboratories, USA). Bacteria were taken with a toothpick from colonies grown overnight on LB plates and inoculated onto swimming plates by stabbing the agar with the toothpick midway and incubated at 30°C for 24 h. Total of 8 different plates were used to calculate swimming potential by measuring the diameter of the bacterial colony formed. Twitching plates contained 1% tryptone (Difco Laboratories, USA), 0.5% yeast extract, 1% NaCl (Merck, Germany), and 1% agar (Difco Laboratories, USA). Bacteria were taken from colonies grown overnight on LB plates and inoculated onto twitching plates by stabbing the agar with the toothpick all through the agar and inoculated at 30°C for 24 h. Total of 10 replicates were used to calculate twitching potential by measuring diameter of bacterial spread at the bottom of the plate after removing the agar and dying the bacteria at the bottom using crystal violet 1% (c0775, Sigma, USA).
4
Cultivation of Extremophilic Bacteria
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D. radiodurans strains were grown at 30 °C in TGY broth composed of 0.5% tryptone (Difco Laboratories, Detroit, MI, USA), 0.3% yeast extract (Difco Laboratories, Detroit, MI, USA), and 0.1% glucose (Sigma-Aldrich, St. Louis, MO, USA) or on TGY plates with 1.5% Bacto-agar (Difco Laboratories, Detroit, MI, USA). E. coli strains, DH5α (Thermo Fisher Scientific, Waltham, MA, USA) and BL21(DE3) (Novagen, Darmstadt, Germany), were cultivated in Luria-Bertani (LB) broth (Difco Laboratories, Detroit, MI, USA) (1% tryptone, 0.5% yeast extract, 1% NaCl) or on LB medium with 1.5% Bacto-agar at 37 °C. Antibiotics were added to the medium if necessary: ampicillin (Sigma-Aldrich, St. Louis, MO, USA), 100 μg/mL (E. coli); kanamycin (Sigma-Aldrich, St. Louis, MO, USA), 50 μg/mL (E. coli) and 8 μg/mL (D. radiodurans); and chloramphenicol (Sigma-Aldrich, St. Louis, MO, USA), 3 μg/mL (D. radiodurans).
5
Differential Agar for Probiotic Strains
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Lactobacillus differential (LBD) agar was used to differentiate P. freudenreichii strains from L. paracasei subsp. paracasei NCC 2511. It contained per litre: 10.0 g of casein enzymatic hydrolysate (Becton Dickinson, Franklin Lakes, NJ, USA), 3.0 g of casein acid hydrolysate (Merck, Darmstadt, Germany), 1.5 g of enzymic digest of soybean meal (Becton Dickinson), 1.0 g of yeast extract (Becton Dickinson), 2.5 g of fructose (Amresco, Solon, OH, USA), 2.5 g of K2HPO4 (Sigma-Aldrich), 55 mg of bromocresol green (Sigma-Aldrich), and 15.0 g of agar (Becton Dickinson) [75 (link)]. In addition, TSB agar was used to grow B. amyloliquefaciens NCC 156 for the estimation of colony forming units. It contained per litre: 17.0 g of tryptone (Becton Dickinson), 5.0 g of NaCl, 3.0 g of soytone (Becton Dickinson), 2.5 g K2HPO4, and 1.0 mL of 30% silicone antifoam, and 15.0 g of agar (Becton Dickinson) [28 ].
6
Peptidyl-pNA Enzymatic Assay
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Chemical reagents such as Tris, glycine, CuCl2·2H2O, ZnCl2, ZnSO4·7H2O, KCl, Na2HPO4·12H2O, NaCl, and KH2PO4 were purchased from Wako Pure Chemical Industries Ltd., Osaka, Japan (Guaranteed Reagent). The synthetic substrate peptidyl‐pNA, benzoyl‐(DL or L)‐arginine‐p‐nitroanilide monohydrochloride (Arg‐pNA), was purchased from Peptides Institute Inc, Osaka, Japan. Tryptone and yeast extract were purchased from Becton‐Dickinson and Company, NJ, USA.
7
Recombinant PD-1 Molecule Characterization
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Chemical reagents such as Tris, glycine, CuCl2·2H2O, KCl, Na2HPO4·12H2O, NaCl, KH2PO4, EDTA·2Na and IPTG were purchased from Wako Pure Chemical Industries Ltd., Osaka, Japan (Guaranteed Reagent). The synthetic substrate peptidyl-pNA, Arg-pNA, was purchased from Peptides Institute Inc., Osaka, Japan. Tryptone and yeast extract were purchased from Becton–Dickinson and Company, NJ, USA. A commercially available recombinant PD-1 molecule was used (ENZO Life sciences Inc., Product Number; ENZ-PRT190; PD-1 (aa 25-167) containing a 5′-His-tag, V5 epitope tag spacer, and FLAG-tag.
8
Cultivation and Enumeration of M. lacticum
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The M. lacticum NBRC14135T strain was obtained from the Biological Resource Center (NBRC), National Institute of Technology and Evaluation, Kisarazu, Japan. Cells were grown at 37 °C with shaking in medium containing 1% (w/v) tryptone (Becton Dickinson and Company, Sparks, MD), 0.5% (w/v) Bact Yeast Extract (Becton Dickinson and Company, Sparks, MD) and 0.5% (w/v) NaCl, pH 7.0 and were stored in a Microbank (PRO-LAB, Round Rock, TX) at −80 °C until use. Cells were subcultured in 200 ml of the culture medium and incubated at 37 °C for 16 h. Cells were collected by centrifugation after filtration through a 1-μm Glass Fibre Membrane (PALL, Port Washington, NY) to remove fine dust contamination in the growth medium. Cells were suspended in 0.9% (w/v) NaCl, which had been filtered through a DISMIC-25 cs disposable syringe filter unit (Advantec, Tokyo, Japan). Colony-forming units (CFUs) were determined by counting colony numbers on 1.5% (w/v) agar medium plates, inoculated with 100-μl aliquots of cultures grown for 2 days at 37 °C.
9
Ebola Virus Control Preparation
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The virus control was prepared by adding 10 µL of EBOV/Mak in a tripartite soil load12 ,13 (link) (102 to 104 TCID50 virus; 0.25% bovine serum albumin [BSA, Sigma], 0.35% tryptone [Becton Dickinson], 0.08% bovine mucin [Sigma]) to 990 µL of VCM. Final concentrations in the control were: virus (102 to 104 TCID50/mL), BSA (0.0025%), tryptone (0.0035%), and mucin (0.0008%). The positive control were diluted in VCM using a ten-fold dilution scheme from 100 (undiluted) to 10−3, and 50 µL of the resulting solutions were added to Vero E6 cells. Cells were scored for CPE 14 days post-inoculation.
10
Devosia riboflavina Cultivation Protocol
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Lumichrome was purchased from Tokyo Chemical Industry (Tokyo, Japan). Riboflavin and dimethyl sulfoxide (DMSO) were purchased from Wako Chemical Industries (Osaka, Japan). Tryptone, Phytone peptone, and yeast extract were purchased from Becton Dickinson (Franklin Lanes, NJ). All other chemicals were commercially sourced and used without further purification. Devosia riboflavina ATCC 9526 (18 (link)) was purchased from the NITE Biological Resource Center.
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