Dual luciferase firefly luciferase renilla luciferase kit

Manufactured by Promega

The Dual luciferase (Firefly luciferase/Renilla luciferase) kit is a lab equipment product that allows for the simultaneous detection and quantification of two different luciferase reporter enzymes within the same sample. It provides a reliable method for normalizing experimental data and comparing gene expression levels across multiple conditions.

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Lab products found in correlation

Luminoskan ascent, by Thermo Fisher Scientific (9 mentions) Prl tk renilla, by Beyotime (3 mentions) Pgmfoxo lu, by Genomed (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)

3 protocols using dual luciferase firefly luciferase renilla luciferase kit

Dual Luciferase Assay in RAW 264.7 Cells

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A pGMFOXO-Lu (Genomeditech, Shanghai, China), a pRL-TK Renilla (Beyotime, Nan-tong, China) and AMPK siRNA or FOXO3a siRNA were transfected into RAW 264.7 cells using Lipofectamine 2000™ reagent (Invitrogen, CA) [66 (link)]. Then cells, lysed by Promega passive lysis buffer, were assayed by using Promega dual luciferase (Firefly luciferase/Renilla luciferase) kit. Luciferase intensity detected with a Luminoskan Ascent (Thermo Fisher Scientific Inc. Finland).

Zhao Y., Sun Y., Ding Y., Wang X., Zhou Y., Li W., Huang S., Li Z., Kong L., Guo Q, & Lu N. (2015). GL-V9, a new synthetic flavonoid derivative, ameliorates DSS-induced colitis against oxidative stress by up-regulating Trx-1 expression via activation of AMPK/FOXO3a pathway. Oncotarget, 6(28), 26291-26307.

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Quantifying FOXO Transcriptional Activity

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A549 cells were seeded in 6-well plate, cultured for 24 h, and then transfected with 1 μg pGMFOXO-Lu (Genomeditech, Shanghai, China) and 0.05 μg pRL-TK Renilla (Beyotime, Nantong, China) with 10 μl Lipofectamine 2000 and incubated for 24 h at 37 ℃ with LZ-101. Cells were lysed with Promega passive lysis buffer and assayed by using Promega dual luciferase (Firefly luciferase/Renilla luciferase) kit. Luciferase intensity was detected with a Luminoskan Ascent (Thermo Fisher Scientific Inc. Finland).

Guo Y., Zhao Y., Zhou Y., Tang X., Li Z, & Wang X. (2019). LZ-101, a novel derivative of danofloxacin, induces mitochondrial apoptosis by stabilizing FOXO3a via blocking autophagy flux in NSCLC cells. Cell Death & Disease, 10(7), 484.

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NF-κB Transcriptional Activity Assay

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H460 cells were seeded in 6-well plate cultured for 12 h and then transfected with 1mg NF-κB -TA-luc (Beyotime, Nan-tong, China) and 0.05 mg pRL-TK Renilla (Beyotime) with 10 mL Lipofectamine 2000 and incubated for 24 h at 37°C with OA. Then cells, lysed by Promega passive lysis buffer, were assayed by using Promega dual luciferase (Firefly luciferase/Renilla luciferase) kit. Luciferase intensity detected with a Luminoskan Ascent (Thermo Fisher Scientific, Inc., Waltham, MA).

Shen L., Zhang L.L., Li H., Liu X., Yu X.X., Hu P., Hui H., Guo Q.L, & Zhang S. (2017). Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer. Oncotarget, 8(30), 49395-49408.

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