Lipofectamine 2000 transfection reagent (11,668–019) was purchased from Life Technologies Co. Invitrogen (Carlsbad, CA, USA). AEG-1/MTDH antibodies (13,860–1-AP) were purchased from Zhongshan Goldenbridge Biotechnology, Inc. (Beijing, China). eIF4E (9472) and cyclin D1(2922) were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Actin antibody (AP0060) was purchased from Bioworld Technology, Inc. (Louis Park, MN, USA).
Cyclin d1 2922
Manufactured by Cell Signaling Technology
Sourced in United States
Cyclin D1(2922) is a primary antibody that targets the cyclin D1 protein. Cyclin D1 is a key regulator of the cell cycle and plays a crucial role in the G1/S phase transition. The antibody can be used for applications such as western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Aeg 1 mtdh antibodies 13 860 1 ap, by Zhongshan Golden Bridge Biotechnology (1 mentions)
α sma, by Abcam (1 mentions)
Interleukin 6 (il 6), by Abcam (1 mentions)
Phospho jak2 tyr1007 1008, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Erk1 2 137f5, by Cell Signaling Technology (1 mentions)
Phospho akt 193h12, by Cell Signaling Technology (1 mentions)
Zeb 1 4c4, by Abnova (1 mentions)
Aldh1a1, by Merck Group (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
Hif 1α, by Cell Signaling Technology (1 mentions)
Nf κb, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions)
Cleaved parp 5625, by Cell Signaling Technology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
Protease and phosphatase inhibitor, by Selleck Chemicals (1 mentions)
β actin 4967, by Cell Signaling Technology (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
4 protocols using cyclin d1 2922
1
Lipofectamine 2000 Transfection Protocol
2
Comprehensive Protein Analysis Protocol
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This has been performed as previously described [32 (link)]. Antibodies directed against alpha smooth muscle actin (α-SMA), Twist-1, Vimentin (RV202), N-cadherin, transforming growth factor beta 1 (TGF-β1), VEGF-A (ab46154) and IL-6 were purchased from Abcam (Cambridge, MA); Lin28B (D4H1), AKT (C73H10), p-AKT (Thr308), STAT3, pSTAT3-Tyr705 (D3A7), Snail (C15D3), E-cadherin (24E10), EpCam (UV1D9), JAK-2 (D2E12), phospho-JAK-2 (TYR1007/1008), Cyclin D1 (2922), Akt, phospho-Akt (193H12), NF-κB, ERK1/2 (137F5), phospho-ERK1/2, MMP-9, HIF-1α, Glyceraldehydes-3-phosphate dehydrogenase (GAPDH, 14C10) and β-Actin from Cell Signaling (Danvers, MA); ZEB-1 (4C4) from Abnova (Taipei, Taiwan), ALDH1A1 from Sigma (CA, USA) and IL-8 (EpR1116(2)) from Origene (Rockville, MD, USA).
3
Western Blot Analysis of Apelin Signaling
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Western blot was performed as previously described 26 (link). Briefly, the total protein was loaded to a SDS-PAGE gel (6% stacking gel and 15% separating gel). After gel electrophoresis, the proteins were transferred to a nitrocellulose membrane at 70V for 2 h and then blocked with 5% bovine serum albumin (BSA) for 30 min at room temperature. Next the blocked membrane was incubated with the primary antibody overnight at 4 °C. Primary antibodies used were APLN (sc-33804), APLNR (sc-33823) and β-actin (sc-47778) (Santa Cruz Biotechnology, Santa Cruz, CA); Phospho-PI3 Kinase p85 (Tyr458) (4228), Akt (9272), phospho-Akt (Ser473) (9271), GSK3β (9315), phospho-GSK3β (Ser9) (9336), Cleaved Caspase-3 (9661), Cleaved PARP (5625) and Cyclin D1 (2922) (Cell Signaling Technology, Danvers, MA). Protein quantification was performed by Image J.
4
Western Blot Analysis of Signaling Proteins
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Cells were harvested using radio-immunoprecipitation assay buffer supplemented with protease and phosphatase inhibitors (Bimake, Houston, TX). Equal amounts of protein were loaded per lane into an SDS-PAGE followed by transferring onto a nitrocellulose membrane (BioRad). The blots were blocked with 5% nonfat dried milk followed by incubation in the respective antibodies. After several washes, membranes were incubated with appropriate secondary antibodies and imaged using either chemiluminescence or the LICOR Odyssey Infrared Imaging System (Lincoln, NE). Antibodies for western blot analysis were used at 1:1000 dilution unless specified otherwise. HA-Tag #sc-7392, Fra-1 #sc-183, ATF-2 #sc-187, GAPDH #sc-137179 were from Santa Cruz Biotechnology; anti-phosphor-MK2 #07-155 from Upstate Biotechnology; Myc-tag #2866, ERK #9102, phosphor-ERK #4376, p38MAPK #9212, phosphor-p38MAPK #4511, JUN #9165, MK2 #3042, Jab1 #9444, Cyclin D1 #2922, βActin #4967 were from Cell Signaling; FLAG #F3165 was from MilliporeSigma; uPA #395 and uPAR #3937 were from American Diagnostica used at 1:500 dilution; Anti-phosphorylated JAB1 (Ser177) pAb was commercially prepared by EZ Biolab (Carmel, IN) using synthesized AVVIDPTRTI(pS)AGKVN peptide and used at 1:500 dilution. All blots derive from the same experiment and were processed in parallel.
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