Angiopoietin 2 (ANGPT2), Angiopoietin-like 4 (ANGPTL4), hepatocyte growth factor (HGF), monocyte chemoattractant protein-1 (MCP-1), pentraxin 3 (PTX3), and VEGF ELISA kits were purchased from R&D systems. Undiluted vitreous samples were analyzed for ANGPT2, ANGPTL4, MCP-1, PTX3 and VEGF, and 1:5 diluted (in PBS) vitreous samples were analyzed for HGF. ELISAs were performed according to the manufacturer’s protocols.
Vegf elisa kit
Manufactured by R&D Systems
Sourced in United States, China, United Kingdom, Germany
The VEGF ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of Vascular Endothelial Growth Factor (VEGF) levels in cell culture supernates, serum, and plasma. The kit utilizes a specific antibody coated on a microplate to capture VEGF, which is then detected using a biotinylated detection antibody and streptavidin-peroxidase conjugate.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Igf 1 elisa kit, by R&D Systems (2 mentions)
In vitro angiogenesis assay kit, by Merck Group (2 mentions)
Mcp 1, by R&D Systems (1 mentions)
Tgf β1 emax immunoassay system, by Promega (1 mentions)
Tgf α, by RayBiotech (1 mentions)
Timp 4, by RayBiotech (1 mentions)
Timp 2, by RayBiotech (1 mentions)
Timp 1, by RayBiotech (1 mentions)
Basic fibroblast growth factor (bfgf), by RayBiotech (1 mentions)
Pdgf bb, by RayBiotech (1 mentions)
Tgfβ1, by RayBiotech (1 mentions)
Growth factor reduced matrigel, by BD (1 mentions)
Flexstation 3, by Molecular Devices (1 mentions)
Pdgf bb elisa kit, by R&D Systems (1 mentions)
Hif1α elisa kit, by R&D Systems (1 mentions)
Triglyceride quantification kit, by Abcam (1 mentions)
Biotek synergy mx microplate reader, by Agilent Technologies (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
73 protocols using vegf elisa kit
1
Vitreous Biomarker ELISA Protocol
2
Regulation of VEGF by miR-29a in Synovial Fibroblasts
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Aliquots of synovial fibroblasts (1 × 105 cells/well, 24-well plate) transfected with miR-29a, antisense oligonucleotide, and scramble control were incubated in basal medium. After incubation for 24 hours, culture media of synovial fibroblasts transfected with miR-29a, antisense oligonucleotide, and scramble control were harvested and centrifuged. The VEGF levels in the culture supernatants were quantified using VEGF ELISA kits (R&D Systems).
3
TGFβ1 and VEGF Quantification Assay
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Different cells were planted with appropriate concentrations, and after 48 h cells would reached ∼80% confluence. The medium was then moved into sterilized tubes. After centrifugation, the supernatant was collected and stored at −80 °C. For neutralizing assay, the supernatant was preincubated with 1 μg ml−1 neutralizing antibody (R&D) for 2 h at room temperature before centrifugation. The concentration of TGFβ1 and VEGF in the supernatant was determined by ELISA. The TGFβ1 Emax ImmunoAssay System was purchased from Promega, and the VEGF ELISA kits were purchased from R&D Systems. The assay was performed according to the manufacturer's protocol.
4
VEGF Quantification in HREC Media
5
Quantifying Wound Healing Factors
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Specific components relevant to wound healing were identified and concentrations of these components were measured using enzyme-linked immunosorbent assay (ELISA) in each sample type. PDGF-BB, PDGF-AA, TIMP-1, TIMP-2, TIMP-4, TGF-β1, TGF-α, bFGF, and EGF ELISA kits were obtained from RayBiotech, Inc (Norcross, GA). Hyaluronan (Hyaluronic acid [HA] ) and VEGF ELISA kits were obtained from R&D Systems (Bio-Techne Corporation, Minneapolis, MN). Lactoferrin ELISA kits were obtained from AssayPro, LLC (St. Charles, MO). Assays were performed according to each kit manufacturer's instructions. Component concentrations in the IL are reported. To compare component concentrations, dCHPM component concentrations were normalized to the component concentrations of fresh, unprocessed PM.
6
Salinomycin and Regorafenib Mechanism Study
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Reagents of Salinomycin (Sal) and Regorafenib (Reg) were purchased from Sigma (St. Louis, MO, USA) and Selleck Chemicals (Shanghai, China), respectively. Both of them was dissolved in 100% DMSO to form a 20 mM solution and stored at −80°C in small aliquots until needed. Growth factor-reduced Matrigel was purchased from BD Biosciences (San Diego, CA). Antibodies against VEGFR2 (2479#), STAT3 (9139#), Bcl2 (#2872), BCL-xl (#2764), Caspase-3(#9664), and phosphor-specific anti-VEGFR2 (Tyr1,175) (#2478) and anti-STAT3 (Tyr705) (#9145) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-CD31 (#ab28364), anti-Ki67 (#ab66155), anti-VEGFR1 (ab32152#) and phosphor-specific anti-VEGFR1 (Y1213) (ab195762#) were provided by Abcam (UK). Recombinant human VEGF (VEGF165) (#293-VE-010) and VEGF ELISA kits (#DVE00) were purchased from R&D Systems (MN, USA). All other reagents were acquired from Sigma-Aldrich (St Louis, MO, USA).
7
Astrocyte Cytokine Response Profiling
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The astrocytes were incubated with the following cytokines: 100 nM SP, 10 ng/mL IL-4, 10 ng/mL IL-10, 10 ng/mL TNF-α, or 20 ng/mL IFN-γ. The conditioned medium was collected 24 h after the cytokine treatment. The CINC-1, CINC-2, and VEGF levels were measured using rat CINC-1, CINC-2, and VEGF ELISA kits (R&D Systems, Minneapolis, MN, USA). Data from three independent experiments were statistically analyzed.
8
Secreted VEGF Quantification
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Transfected cells were seeded into six-well plates, and after 48 h the culture supernatants were collected and the particulates were removed by centrifugation. The numbers of cells in each plate were counted. The protein levels of VEGF were measured using VEGF ELISA kits (R&D Systems, Minneapolis, MN) according to the manufacturer's instructions. The results were normalized by each cell numbers.
9
ARPE-19 Cells Treated with Chemotherapeutics
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ARPE-19 cells were treated with 5 mg/mL 5-Fu, 0.02 mg/mL MMC, 2.5 mg/mL BVZ, 5 mg/mL 5-Fu + 2.5 mg/mL BVZ, 0.02 mg/mL MMC + 2.5 mg/mL BVZ, or PBS as the control. After 24 h, 200 μL of the supernatant per well was collected and analyzed using a VEGF ELISA Kit (R&D Systems) according to the manufacturer's protocol [28 (link)].
10
VEGF-A Secretion Quantification
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ELISA was used to detect secreted VEGF-A in vascular SMC culture media using a VEGF ELISA kit based on the sandwich enzyme immunoassay technique (R&D Systems, Minneapolis, MN, USA). The absorbance was determined using a Flex Station 3 microplate reader (Molecular Devices, Sunnyvale, CA, USA).
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