Anti decorin

Manufactured by Thermo Fisher Scientific

Sourced in Mongolia

Anti-decorin is a laboratory reagent used to detect and quantify the presence of decorin, a small leucine-rich proteoglycan, in biological samples. It can be utilized in various research applications that involve the study of decorin expression and function.

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Lab products found in correlation

Anti lumican, by R&D Systems (1 mentions) Anti cd44, by Thermo Fisher Scientific (1 mentions) Anti perilipin, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 or 594 conjugated donkey igg, by Thermo Fisher Scientific (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) Bx41 microscope, by Olympus (1 mentions) Optimal cutting temperature compound, by Sakura Finetek (1 mentions)

2 protocols using anti decorin

Immunofluorescence Analysis of Healthy Human Kidney

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Cryosections of nephrectomized healthy human kidney tissue was used for immunofluoresence. The antibodies used were; anti-lumican (R&D Systems, Minneapolis, MN), anti-decorin (Thermo Scientific), anti-glypican-4 (Thermo Scientific), anti-CD44 (Thermo Scientific) anti-agrin (Abcam), anti-collagen alpha-1 (XVIII) chain and collagen alpha-1 (XV) chain (Atlas Antibodies, Stockholm, Sweden). For endothelial co-localization the endothelial-specific lectin rhodamine Ulex Europeaus agglutinin (Vector Laboratories, Burlingame, CA) was used and the sections were analyzed with a confocal microscope, Zeiss (Zeiss, Oberkochen, Germany).

Khramova A., Boi R., Fridén V., Granqvist A.B., Nilsson U., Tenstad O., Oveland E., Haraldsson B., Ebefors K, & Nyström J. (2021). Proteoglycans contribute to the functional integrity of the glomerular endothelial cell surface layer and are regulated in diabetic kidney disease. Scientific Reports, 11, 8487.

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Immunostaining of 3D Collagen Cultures

Cited 1 time

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Cells were fixed in −20 °C cold methanol for 10 minutes then blocked with phosphate buffered saline containing 5% donkey serum at room temperature for 30 minutes. For sectioning of 3D collagen 3T3-L1, collagen plug was embedded in Optimal Cutting Temperature compound (Sakura Finetek USA, Torrance, CA) as previously described (Lee et al., 2004 (link)). Sectioning was performed at University of California–San Diego Moores Histology Core (La Jolla, CA). The detailed immunostaining method has been described (Sato et al., 2016 (link)). Anti-pCREB, anti-perilipin, anti-decorin, and Alexa Fluor 488- or 594-conjugated donkey IgG (Thermo Fisher Scientific) were used at 1μg/ml. Cover slips were mounted using ProLong Gold Antifade Reagent with DAPI (Thermo Fisher Scientific). Images were captured using a BX41 microscope (Olympus, Center Valley, PA).

Sato E., Zhang L.J., Dorschner R.A., Adase C.A., Choudhury B.P, & Gallo R.L. (2017). Activation of Parathyroid Hormone 2 Receptor Induces Decorin Expression and Promotes Wound Repair. The Journal of investigative dermatology, 137(8), 1774-1783.

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