Triiodo l thyronine

Manufactured by Merck Group

Sourced in United States, France

Triiodo-L-thyronine is a laboratory reagent used to measure thyroid hormone levels in biological samples. It is the active form of thyroid hormone and plays a crucial role in regulating metabolism, growth, and development.

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Lab products found in correlation

Insulin, by Merck Group (13 mentions) Hydrocortisone, by Merck Group (10 mentions) Adenine, by Merck Group (8 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (6 mentions) Prostaglandin e1, by Merck Group (6 mentions) Cholera toxin, by Merck Group (5 mentions) Sodium selenite, by Merck Group (5 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions) Dmem f12 media, by Merck Group (4 mentions) Dexamethasone (dex), by Merck Group (4 mentions) Ascorbic acid, by Merck Group (4 mentions) Transferrin, by Merck Group (4 mentions) Geneticin, by Thermo Fisher Scientific (4 mentions) Recombinant human egf, by Thermo Fisher Scientific (4 mentions) Fetal bovine serum (fbs), by Merck Group (3 mentions) Ham s f12, by Thermo Fisher Scientific (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) L thyroxine, by Merck Group (3 mentions) Poly l lysine (pll), by Merck Group (3 mentions) D biotin, by Merck Group (3 mentions)

28 protocols using triiodo l thyronine

Beige and Brown Adipocyte Differentiation

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The isolated cells were cultured in DMEM/F12 media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich) and 1% Penicillin/Streptomycin (Gibco) at 37 °C in a 5% CO₂ humidified incubator. For beige and brown adipocyte differentiation, post-confluent cells were induced by DMEM/F12 containing 10% FBS, 0.5 mM isobutylmethylxanthine (Sigma-Aldrich), 1 µg/ml insulin (Sigma-Aldrich), 1 µM dexamethasone (Sigma-Aldrich), 60 µM indomethacin (Sigma-Aldrich), 1 nM triiodo-L-thyronine (Sigma-Aldrich), and 1 µM rosiglitazone (Sigma-Aldrich) for 2 days and with DMEM/F12 containing 10% FBS and 1 µg/ml insulin, 1 nM triiodo-L-thyronine, and 1 µM rosiglitazone every 2 days. Fresh medium was replaced every 2 days until ready for harvest. To withdraw external stimuli, the differentiation medium was changed to DMEM containing 5% BSA for 4 days.

Wu R., Park J., Qian Y., Shi Z., Hu R., Yuan Y., Xiong S., Wang Z., Yan G., Ong S.G., Song Q., Song Z., Mahmoud A.M., Xu P., He C., Arpke R.W., Kyba M., Shu G., Jiang Q, & Jiang Y. (2023). Genetically prolonged beige fat in male mice confers long-lasting metabolic health. Nature Communications, 14, 2731.

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Beige Adipogenesis Induction Protocol

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The isolated SV cells were cultured in DMEM/F12 media (Sigma-Aldrich, St. Louis, MO) supplemented with 10% FBS (Sigma-Aldrich, St. Louis, MO) and 1% Penicillin/Streptomycin (Gibco, Waltham, MA). Beige adipogenesis was induced by treating confluent cells with DMEM/F12 containing 10% FBS, 1 μg/mL insulin (Sigma-Aldrich, St. Louis, MO), 1 μM dexamethasone (Cayman, Ann Arbor, MI), 0.5 mM isobutylmethylxanthine (Sigma-Aldrich, St. Louis, MO), 60 μM indomethacin (Sigma-Aldrich, St. Louis, MO), 1 nM triiodo-L-thyronine (Sigma-Aldrich, St. Louis, MO), and 1 μM rosiglitazone (Sigma-Aldrich, St. Louis, MO) for the first 3 days and with DMEM/F12 containing 10% FBS and 1 μg/mL insulin, 1 nM triiodo-L-thyronine, and 1 μM rosiglitazone for every 3 days. To induce thermogenic genes, cells were treated with 5 μM CL316,243 for 4 days.

Park J., Shin S., Liu L., Jahan I., Ong S.G., Xu P., Berry D.C, & Jiang Y. (2021). Progenitor-like characteristics in a subgroup of UCP1+ cells within white adipose tissue. Developmental cell, 56(7), 985-999.e4.

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Cell Culture Protocols for Prostate and Cancer Cell Lines

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All cell lines were obtained from ATCC. PC3 and DU145 cells were cultured in F-12K medium and Eagle’s minimum essential medium supplemented with fetal bovine serum (FBS) to a final concentration of 10%, respectively. C4-2B cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 (4:1) supplemented with heat-inactivated FBS (to 10%) and T-medium supplement (to 1×). To prepare 10×T-medium supplement in 100 ml of 0.1% BSA (Sigma cat# A8022) in PBS, the following components were added: 50 mg insulin (Gibco cat# 12585-014), 136 ng triiodo-l-thyronine (Sigma cat# T2877), 50 mg transferrin (Sigma cat# T4382), 2.5 mg d-Biotin (Sigma cat# 47868), and 250 mg adenine (Sigma cat# A3159). RWPE-1 and PZ-HPV-7 cells were maintained in keratinocyte serum free medium (K-SFM, Gibco 17005-042), supplemented with 0.05 mg/ml of bovine pituitary extract and 5 ng/ml of human recombinant epidermal growth factor. HPrEC cells were maintained in prostate epithelial cell basal medium (ATCC, PCS-440-030) supplemented with prostate epithelial cell growth kit (ATCC, PCS-440-040). HeLa cells were cultured in DMEM supplemented with 5% FBS.

Wu R.C., Young I.C., Chen Y.F., Chuang S.T., Toubaji A, & Wu M.Y. (2019). Identification of the PTEN-ARID4B-PI3K pathway reveals the dependency on ARID4B by PTEN-deficient prostate cancer. Nature Communications, 10, 4332.

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Cultivation of Immortalized Human Renal Proximal Tubular Epithelial Cells

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PTEC-TERT1 cells (renal PTEC [RPTEC]/TERT1, CHT-003-0002; Evercyte, Wien, Austria) were cultured according to the manufacturer’s instructions in PTEC medium: Mix 1/1 of DMEM (#11966-025) and Ham’s F-12 nutrients (#21765-29) (Thermo Fisher Scientific, Waltham, MA, USA) containing 1% penicillin-streptomycin (GIBCO, #15140-122), 10 mM HEPES (GIBCO, #15630-056), 5.0 μg/mL human insulin (GIBCO, #41400-045), 5.0 μg/mL human transferrin (GIBCO, #41400-045), 8.65 ng/mL sodium selenite (GIBCO, #41400-045), 0.1 μM hydrocortisone (#H6909, Sigma-Aldrich, St. Louis, MO, USA), 10 ng/mL human recombinant EGF (#236-EG-200, R&D Systems, Minneapolis, MN, USA), 3.5 μg/mL ascorbic acid (Sigma-Aldrich, #A4544 powder), 25 ng/mL prostaglandin E1 (Sigma-Aldrich, #P5515), 3.2 pg/mL Triiodo-L-thyronine (Sigma-Aldrich, #T-5516), and 100 μg/mL Geneticin (GIBCO, #10131-027).

Sewing S., Gubler M., Gérard R., Avignon B., Mueller Y., Braendli-Baiocco A., Odin M, & Moisan A. (2018). GalNAc Conjugation Attenuates the Cytotoxicity of Antisense Oligonucleotide Drugs in Renal Tubular Cells. Molecular Therapy. Nucleic Acids, 14, 67-79.

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Pancreatic Acinar Cell and Organoid Culture

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All cells were cultured at 37 °C with 5% CO₂. Primary pancreatic acinar cells from mice were cultured in Waymouth Complete Media (Waymouth MB 752/1 Medium with 1% FBS, 0.1 mg/ml trypsin inhibitor, and 1 μg/ml dexamethasone). Culture media for primary PanIN organoids consisted of DMEM/F12 media (Sigma-Aldrich) containing 5% FBS, 25 μg/ml bovine pituitary extract (Gibco/Thermo Scientific, Waltham, MA), 20 ng/ml EGF, 0.1 mg/ml soybean trypsin inhibitor type I (AMRESCO, Solon, OH), 5 mg/ml D-glucose (Sigma-Aldrich), 1.22 mg/ml nicotinamide (Sigma-Aldrich), 5 nM triiodo-L-thyronine (Sigma-Aldrich), 1 μM dexamethasone (Sigma-Aldrich), 100 ng/ml cholera toxin (Sigma-Aldrich), 5 ml/l insulin-transferrin-selenium (Corning) and 100 U/ml penicillin/streptomycin (Gibco/Thermo Scientific). Primary peritoneal macrophages were cultured in RPMI-1640 containing 10% FBS and 1% Pen/Strep. Male and female mice were used in approximately equal numbers for all primary cell isolations.

Liou G.Y., Fleming Martinez A.K., Döppler H.R., Bastea L.I, & Storz P. (2023). Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth. iScience, 26(6), 106820.

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Directed Cardiac Differentiation of hiPSCs

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hiPSCs (>p20) were split at 1:12 to 1:15 ratios using EDTA as above and grown for 3–4 days at which time they reached ~75% confluence. Media was changed to CDM3^{6 (link)}, consisting of RPMI 1640 (10–040–CM, Corning), 500 µg ml⁻¹Oryza sativa–derived recombinant human albumin (Oryzogen Sciencell), and 213 µg ml⁻¹L–ascorbic acid 2–phosphate (Sigma–Aldrich). Media was changed every other day (48 h). For days 0–2, media was supplemented with 6 µM CHIR99021 (MedChem Express)^{19 , 20}. On day 2, media was changed to CDM3 supplemented with 2 µM Wnt–C59 (Biorbyt). Media was changed on day 4 and every other day for CDM3. Contracting cells were noted from day 7. At day 10, media was changed to CDM3L made with using RPMI 1640 no glucose (11879–020, Life Technologies), 500 µg ml⁻¹ recombinant human albumin, and 213 µg ml⁻¹L–ascorbic acid 2–phosphate supplemented with 4 mM L–lactic acid (Sigma–Aldrich). At day 15, media was changed to CDM3M consisting of RPMI 1640 no glucose, 500 µg ml⁻¹ recombinant human albumin, 213 µg ml⁻¹L–ascorbic acid 2–phosphate supplemented with 10 mM D–galactose (Sigma–Aldrich)^{21 (link)}, 4 mM L–lactic acid, 1 mM sodium pyruvate (Life Technologies), 20 µg ml⁻¹ insulin (Life technologies), 1 × chemically defined lipid concentrate (Life Technologies), and 200 ng ml⁻¹ tri–iodo–L–thyronine (Sigma–Aldrich)^{22 (link)}.

Burridge P.W., Li Y.F., Matsa E., Wu H., Ong S., Sharma A., Holmström A., Chang A.C., Coronado M.J., Ebert A.D., Knowles J.W., Telli M.L., Witteles R.M., Blau H.M., Bernstein D., Altman R.B, & Wu J.C. (2016). Human Induced Pluripotent Stem Cell–Derived Cardiomyocytes Recapitulate the Predilection of Breast Cancer Patients to Doxorubicin–Induced Cardiotoxicity. Nature medicine, 22(5), 547-556.

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Differentiation of Keratinocyte Stem Cells

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After cell sorting, KSCs and TAs were seeded at 5.10⁵/cm² in culture inserts 0.4 μm PCF (Millicell, Millipore) in keratinocyte medium (DMEM and Ham’s F12 at a ratio of 3:1 (Thermo Fisher Scientific)) supplemented with 10% fetal calf serum (Hyclone, Logan, Utah, USA), 10 ng/mL epidermal growth factor (EGF) (Sigma, St Quentin Fallavier, France), 24.3 μg/mL adenine (Sigma), 0.4 μg/mL hydrocortisone (Upjohn, Serb Laboratories, Paris, France), 0.12 IU/mL insulin (Lilly France, St Cloud, France), 2.10-9M triiodo-L-thyronine (Sigma), 10⁻⁹ M cholera toxin (Sigma) and antibiotics. The next day, they were irradiated at 10 J/cm² as previously described. Cells were then cultured in a keratinocyte medium for 7 days in immerged conditions before elevation at air/liquid interface for 7 additional days in a differentiation medium (DMEM supplemented with 8 mg/mL bovine serum albumin (Sigma, St Quentin Fallavier, France), 0.12 IU/mL insulin, 0.4 μg/mL hydrocortisone, and antibiotics). Experiments were performed on samples from three donors.

Metral E., Bechetoille N., Demarne F., Damour O, & Rachidi W. (2018). Keratinocyte stem cells are more resistant to UVA radiation than their direct progeny. PLoS ONE, 13(9), e0203863.

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Culturing RPTEC/TERT1 Cells with Supplements

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RPTEC/TERT1 (ATCC, Manassas, VA) were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) (Thermo Fisher Scientific, Waltham, MA), containing L-glutamine and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and supplemented with 10 ng/mL of recombinant human Epidermal growth factor (EGF) (Thermo Fisher Scientific, Waltham, MA), 25 ng/mL prostaglandin E1 (Sigma-Aldrich, St. Louis, MO), 25 ng/mL hydrocortisone (Sigma-Aldrich, St. Louis, MO), 3.5 μg/mL L-ascorbic acid (Sigma-Aldrich, St. Louis, MO), 5 pM triiodo-L-thyronine (Sigma-Aldrich, St. Louis, MO), 1x Insulin-Transferrin-Selenium (ITS-G) (Thermo Fisher Scientific, Waltham, MA) and 100 μg/mL geneticin (Thermo Fisher Scientific, Waltham, MA). Cells were mycoplasma negative and were used up to 16 passages.

Adelfio M., Bonzanni M., Levin M, & Kaplan D.L. (2022). Impact of membrane voltage on formation and stability of human renal proximal tubules in vitro. ACS biomaterials science & engineering, 8(3), 1239-1246.

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Cell Culture and Transfection Protocols

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The cell line Oli-neu was cultured in modified Sato media [28 (link)]. Cell culture dishes were coated with poly-L-lysine (Sigma). HEK293T cells were cultured in DMEM (Sigma) with 10% Horse serum (Biochrom) and 1% Sodium-pyruvate (Sigma). Transfection of HEK293T cells with the NG2del constructs was effected by a standard protocol using the GenePulserXcell (Bio-Rad). Transcription was increased by including 4 mM sodium butyrate for the protease assay.
Cerebella of postnatal day 8–9 homozygous NG2-EYFP(NG2-KO) mice [29 (link)] or C57BL/6N mice (as control) were dissociated in 1% trypsin, 0.05% DNase in HBSS using a fire-polished Pasteur pipette to obtain a single-cell suspension, followed by seeding on poly-L-lysine-coated dishes. The cells were cultured in B27 medium containing DMEM, pyruvate, triiodo-L-thyronine, L-thyroxine (Sigma), B27 supplement (Gibco), 10ng/ml PDGF, 5ng/ml FGF (PrepoTech) and 1% HS. The medium was changed on the following day and renewed every 3–4 d. After 10-14d (after morphological assessment) cultures were stressed with H₂O₂ in B27 medium without growth factors.
Glioblastoma cells (R10) were cultured on ECM (Sigma) gel-coated dishes. ECM Gel was diluted 1/10 with Neurobasal media. Growth medium was Neurobasal medium (Invitrogen) with N2 supplement, B27 supplement, L-glutamine, EGF, FGF and 1% [v/v] penicillin/streptomycin (Serva).

Maus F., Sakry D., Binamé F., Karram K., Rajalingam K., Watts C., Heywood R., Krüger R., Stegmüller J., Werner H.B., Nave K.A., Krämer-Albers E.M, & Trotter J. (2015). The NG2 Proteoglycan Protects Oligodendrocyte Precursor Cells against Oxidative Stress via Interaction with OMI/HtrA2. PLoS ONE, 10(9), e0137311.

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Expansion of TERT1 Renal Proximal Tubule Cells

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TERT1 human renal proximal tubule epithelial cells (RPTECs) were purchased from ATCC (CRL-4031, Manassas, VA) and immediately cultured upon receipt. Complete growth media was prepared using DMEM:F12 media (ATCC, 30-2006), triiodo-L-thyronine (Sigma, T6397), recombinant human EGF (Life Technologies, PHG0311), ascorbic acid (Sigma, A4403), human transferrin (Sigma, T8158), insulin (Sigma I9278), prostaglandin E1 (Sigma, P7527), hydrocortisone (Sigma, H0888), sodium selenite (Sigma, S5261), and G418 (Sigma, A1720). Complete media was stored at 4°C and used within 28 days. TERT1 RPTECs were grown in CO₂ incubator at 37°C with an atmosphere of 95% air/5% CO₂.

Davis S.A., Vincent B.M., Endo M.M., Whitesell L., Marchillo K., Andes D.R., Lindquist S, & Burke M.D. (2015). Non-toxic antimicrobials that evade drug resistance. Nature chemical biology, 11(7), 481-487.

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