Balb 3t3 clone a31

The BJ (human fibroblast), Balb/3T3 Clone A31 (murine fibroblast) and THP-1 (human monocyte) cell lines were obtained from the American Type Culture Collection (ATCC) located in Manassas, VA, USA.
The BJ, Balb/c 3T3 and THP-1 cells were cultured in MEM containing 10% FBS and 1% Penicillin/Streptomycin, DMEM containing 10% BCS and 1% Penicillin/Streptomycin, and RPMI 1640 containing 10% FBS, 1% Penicillin/Streptomycin and 0.05% β-Mercapto-ethanol, respectively.
All cell lines were maintained at a temperature of 37 °C in a modified atmosphere composed of 5% humidified CO₂.

PRX-VBn consisting of α-CDs and PEG (M_n = 5000) capped with 4-vinylbenzyl groups was synthesized as previously described by Arisaka et al. [13 (link)] (Figures S2 and S3 in Supporting Information). Iodomethane (MeI) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Methanol (MeOH) was purchased from Kanto Chemical (Tokyo, Japan). Collagen sponges for 35 mm culture dishes were purchased from Koken (Tokyo, Japan). Fetal bovine serum (FBS) was purchased from Gibco BRL, Life Technologies (Carlsbad, CA, USA). Ammonium persulfate (APS), butorphanol tartrate, dimethyl sulfoxide (DMSO), Dulbecco’s modified Eagle’s medium (high glucose, with L-glutamine, phenol red, and sodium pyruvate) (D-MEM), 100 U/mL penicillin, 100 µg/mL streptomycin, medetomidine hydrochloride, midazolam, phosphate buffered saline (PBS), sodium hydroxide (NaOH) and N,N,N’,N’-tetramethylene diamine (TEMED) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). The BALB/3T3 clone A31, a fibroblast cell line established from mouse embryos, was obtained from the American Type Culture Collection (Manassas, VA, USA). BALB/c mice were obtained from Sankyo Labo Service (Tokyo, Japan).

Both aAPC/mOKT3 and aAPC/A2 were derived from the human erythroleukemia cell line K562. The aAPC/mOKT3 expresses a membranous form of the anti-CD3 mAb (clone OKT3), as well as the costimulatory molecules CD80 and CD83^{25 (link)}. The aAPC/A2 expresses HLA-A2, CD80, and CD83^{27 (link)}. The human acute lymphoblastic leukemia cell line NALM6 was obtained from DSMZ (Braunschweig, Germany). The murine B-cell lymphoma cell line A20 and BALB/3T3 clone A31 were obtained from the American Type Culture Collection (ATCC) (Manassas, VA). All cells were routinely checked for the presence of mycoplasma contamination using PCR-based technology.