Immunoblotting was performed as previously described17 . Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease inhibitors. Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane. After a blocking step, membranes were incubated with primary antibody overnight at 4°C. Membranes were washed and incubated with secondary antibody conjugated to Li-Cor IRDye for 1 h and visualized using Odyssey infrared imaging (Li-Cor Biosciences, Lincoln, NE). The antibodies used were anti-PPARγ (Santa Cruz, Dallas, Texas, sc-7273), anti-FABP4 (laboratory stock), anti-CD36 (R&D Systems, Minneapolis, MN, AF2519), anti-Perilipin1 (American Research Products, Waltham, MA, 03-651156), anti-sulfenic acid modified cysteine (Milipore, Darmstadt, Germany, ABS30) and anti-β-actin (Sigma-Aldrich, St. Louis, MO, A3854).
Anti sulfenic acid modified cysteine
Manufactured by Merck Group
Anti-sulfenic acid modified cysteine is a lab equipment product. It is a chemical compound used for the analysis and detection of sulfenic acid modifications in proteins.
Automatically generated - may contain errors
2 protocols using anti sulfenic acid modified cysteine
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Immunoblotting for Protein Analysis
2
Immunoblotting for Protein Analysis
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Immunoblotting was performed as previously described17 . Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease inhibitors. Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membrane. After a blocking step, membranes were incubated with primary antibody overnight at 4°C. Membranes were washed and incubated with secondary antibody conjugated to Li-Cor IRDye for 1 h and visualized using Odyssey infrared imaging (Li-Cor Biosciences, Lincoln, NE). The antibodies used were anti-PPARγ (Santa Cruz, Dallas, Texas, sc-7273), anti-FABP4 (laboratory stock), anti-CD36 (R&D Systems, Minneapolis, MN, AF2519), anti-Perilipin1 (American Research Products, Waltham, MA, 03-651156), anti-sulfenic acid modified cysteine (Milipore, Darmstadt, Germany, ABS30) and anti-β-actin (Sigma-Aldrich, St. Louis, MO, A3854).
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