N acetyl l cysteine nac

Manufactured by Abcam

Sourced in United Kingdom, Macao, United States

N-acetyl-L-cysteine (NAC) is a synthetic derivative of the amino acid L-cysteine. It serves as a source of sulfhydryl (thiol) groups and has antioxidant properties.

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Lab products found in correlation

3 protocols using n acetyl l cysteine nac

Antibody-based detection of NLRP3 inflammasome

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Anti-NLRP3 (1:1,000 dilution; cat. no. 19771-1-AP) antibody was purchased from ProteinTech Group, Inc. (Chicago, IL, USA). Anti-ASC antibody (1:1,000 dilution; cat. no. ST1121) and anti-caspase-1 (p20) antibody (1:1,000 dilution; cat. no. AP1043) were purchased from Chemicon (EMD Millipore, Billerica, MA, USA). Anti-β-actin antibody (1:10,000 dilution; cat. no. 13E5) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), and pro-IL-18 and IL-18 (1:1,000 dilution; cat. no. BAF-1520) pro-IL-1β and IL-1β (1:1,000 dilution; cat. no. AF-401-SP) antibodies were purchased from R&D Systems (Minneapolis, MN, USA). Anti-NeuN (1:500 dilution; cat. no. ab177487) and ROS inhibitor N-acetyl-L-cysteine (NAC; 1:1,000 dilution; cat. no. ab143032) were purchased from Abcam (Cambridge, UK). ELISA kits for IL-1β/18 were purchased from USCN Life Sciences, Inc. (Wuhan, China).

Zhuang W., Wang C., Shi X., Qiu S., Zhang S., Xu B., Chen M., Jiang W., Dong H, & Qiao Y. (2018). MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss. International Journal of Molecular Medicine, 41(6), 3448-3456.

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Peroxisome Proliferator-Activated Receptor Agonists and Antioxidants

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Peroxisome proliferator-activated receptor (PPAR) agonists fenofibrate, troglitazone, bezafibrate, 15-deoxy-Δ^12,14-Prostaglandin J₂ (PGJ2), ciglitazone, rosiglitazone, pioglitazone, and MCC-555 were from Cayman Chemical (Ann Arbor, MI, USA). Antioxidants 2,2,5,7,8-Pentamethyl-6-chromanol (PMC), 4-hydroxy-TEMPO (TEMPO, a superoxide dismutase mimic) and Tiron were from Sigma (St. Louis, MO), MCI-186 (MCI), Trolox and DL-a-lipoic acid (lipoic acid) were from Cayman Chemical, N-Acetyl-L-cysteine (NAC) was from Abcam (Cambridge, MA), and elamipretide (ELAM) was from Muse Chem (Fairfield, NJ). Trolox amide analogs (669, 738, 829) were from ChemBridge (San Diego, CA). Inflammasome inhibitors MCC950 and Isoliquiritigenin were from Cayman Chemical. The NADPH-oxidases inhibitor apocynin (APO) was from Abcam and diphenyleneiodonium (DPI) was from Sigma.

Gnanaguru G., Mackey A., Choi E.Y., Arta A., Rossato F.A., Gero T.W., Urquhart A.J., Scott D.A., D’Amore P.A, & Ng Y.S. (2021). Discovery of sterically-hindered phenol compounds with potent cytoprotective activities against ox-LDL–induced retinal pigment epithelial cell death as a potential pharmacotherapy. Free radical biology & medicine, 178, 360-368.

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Inflammatory Cytokine and Hypoxia Signaling

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Recombinant human TNFα and IL-1β were purchased from PeproTech (Cranbury, NJ, USA), and LPS (from E. coli O111:B4) was purchased from InvivoGen (Toulouse, France). N-(2-methoxy-2-oxoacetyl) glycine methyl ester (DMOG; Sigma-Aldrich, St. Louis, MO, USA) and N-acetyl-L-cysteine (NAC; Abcam, Cambridge, UK) were dissolved in ultrapure water, LY294002 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), PD98059 (FUJIFILM), and SC-514 (Sigma-Aldrich) in dimethyl sulfoxide. Echinomycin was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Table A1 provides a list of the reagents and supplies utilized in this study.

Hashimoto Y., Tsuzuki-Nakao T., Kida N., Matsuo Y., Maruyama T., Okada H, & Hirota K. (2023). Inflammatory Cytokine-Induced HIF-1 Activation Promotes Epithelial–Mesenchymal Transition in Endometrial Epithelial Cells. Biomedicines, 11(1), 210.

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