Bright-field images were taken using a Zeiss Axioplan 2+ microscope, Nikon Digital Sight DS-F12 camera, and Nikon NIS 4.0 imaging software. Images of immunohistochemistry and immunostaining of electroporated brains and dissociated cultures were obtained using a Zeiss LSM 5 Exciter–AxioImager M1 imaging system and Zeiss LSM710 imaging system. Image stacks were generated by scanning at intervals of 0.5–1 μm using filters of the appropriate wavelengths. The stacks were analyzed, merged, and projected using ImageJ software (RRID: SCR_003070 ) from the National Institutes of Health. Figure panels were prepared using Adobe Photoshop CS6. Figure 2C,D shows stitched composites from multiple confocal image frames.
Lsm710 imaging system
Manufactured by Zeiss
Sourced in Germany
The LSM710 is a laser scanning microscope system designed for advanced imaging applications. It provides high-resolution optical sectioning and 3D imaging capabilities. The system utilizes multiple laser sources and a sensitive detection system to capture detailed images of biological samples. The core function of the LSM710 is to enable comprehensive visualization and analysis of cellular and subcellular structures.
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Lab products found in correlation
3 protocols using lsm710 imaging system
1
Confocal Imaging of Electroporated Brain Samples
2
Confocal Imaging of Neuronal Structures
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bright-field images were taken using a Zeiss Axioplan 2+ microscope, Nikon Digital Sight DS-F12 camera, and Nikon NIS 4.0 imaging software. Images of immunohistochemistry and immunostaining of electroporated brains and dissociated cultures were obtained using a Zeiss LSM 5 Exciter–AxioImager M1 imaging system and Zeiss LSM710 imaging system. Image stacks were generated by scanning at intervals of 0.5–1 μm using filters of the appropriate wavelengths. The stacks were analyzed, merged, and projected using ImageJ software (RRID: SCR_003070 ) from the National Institutes of Health. Figure panels were prepared using Adobe Photoshop CS6. Figure 2 C,D shows stitched composites from multiple confocal image frames.
3
Immunohistochemical Analysis of AhR Expression
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Ileal tissues were fixed in 4% paraformaldehyde overnight, dehydrated, and
embedded in paraffin. Tissue slices (5 µm in thickness) were mounted on
positively charged glass and dewaxed. Ag retrieval was achieved by incubation in
0.01 M sodium citrate buffer, pH 6.0, for 20 min in a boiling steamer. Slides
were then blocked with normal goat serum blocking reagent (Invitrogen) for
30 min and subjected to sequential incubation with anti-AhR (1:50, ab84833,
Abcam) at 4℃ overnight and fluorophore-conjugated secondary Abs at 24℃ for 2 h
and nuclei were visualized with 4[prime],6-diamidino-2-phenylindole (1:2000,
ab104139, Abcam). Between each incubation step, the slides were washed with PBS
three times for 5 min each. Sections were mounted using fluorescent mounting
medium. Confocal images were obtained with a Zeiss (Oberkochen, Germany) LSM 710
imaging system.
embedded in paraffin. Tissue slices (5 µm in thickness) were mounted on
positively charged glass and dewaxed. Ag retrieval was achieved by incubation in
0.01 M sodium citrate buffer, pH 6.0, for 20 min in a boiling steamer. Slides
were then blocked with normal goat serum blocking reagent (Invitrogen) for
30 min and subjected to sequential incubation with anti-AhR (1:50, ab84833,
Abcam) at 4℃ overnight and fluorophore-conjugated secondary Abs at 24℃ for 2 h
and nuclei were visualized with 4[prime],6-diamidino-2-phenylindole (1:2000,
ab104139, Abcam). Between each incubation step, the slides were washed with PBS
three times for 5 min each. Sections were mounted using fluorescent mounting
medium. Confocal images were obtained with a Zeiss (Oberkochen, Germany) LSM 710
imaging system.
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