Microvette 100 potassium edta blood collection tubes

To evaluate the safety of the micromotor formulation, MC38 tumor-bearing C57BL/6NHsd mice were intratumorally treated with 1 mg of Motor-OMV or PBS (pH 5) when tumor sizes reach approximately 50 mm² on day 10 post-inoculation. After 24 h, blood was collected via the submandibular vein and allowed to coagulate. The serum for chemistry analysis was then collected by centrifuging the coagulated blood at 3,000 g for 5 min. A separate small aliquot of blood was collected into Microvette 100 potassium-EDTA blood collection tubes (Sarstedt) for hematological analysis. All tests were performed by the Animal Care Program Diagnostic Services Laboratory at UCSD. To monitor serum cytokine levels, MC38 tumor-bearing mice were intratumorally administered with 1 mg of Motor-OMV or an equivalent amount of free OMVs once the tumors reached approximately 50 mm² on day 10. Blood was collected via the submandibular vein at 6 h, 24 h, and 48 h after treatment, and the serum was derived as before. Serum from unmanipulated tumor-bearing mice was used to establish a baseline. The samples were stored at −20 °C until ELISA analysis (Table S1, Supporting Information).

Free OMVs or OM-NDs were administered into mice via the hock at a protein dose of 0.5 μg. After 1 day, blood was collected via submandibular puncture into Microvette 100 potassium-EDTA blood collection tubes (Sarstedt) for cell counting. For serum biochemistry analysis, blood was collected without an anticoagulant and allowed to coagulate for at least 30 min before centrifuging at 3000 g for 10 min. All tests were performed by the Animal Care Program Diagnostic Services Laboratory at UCSD.