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3 protocols using sodium phosphate monobasic dihydrate

1

Determination of Peroxidase Activity in Fishery Products

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Hydrogen peroxide (H2O2, 30%), horseradish peroxidase (HRP, freeze-dried powder, >200 units/mg), N,N-diethyl-1,4-phenylenediammonium sulfate (DPD), L-tyrosine and L-phenylalanine were purchased from Aladdin (Shanghai, China). FeCl3 and Nafion (10% in water) were purchased from Sigma-Aldrich (Shanghai, China). Tetrakis(4-carboxyphenyl)porphyrin (H2TCPP) was purchased from TCI (Shanghai, China). Oxalic acid dehydrate was purchased from Mackin (Shanghai, China). ZrClO2·8H2O, benzoic acid, DMF, glucose, ascorbic acid, potassium dihydrogen phosphate, lithium carbonate, sodium phosphate monobasic dihydrate, sodium phosphate dibasic dodecahydrate, magnesium sulfate heptahydrate, and calcium chloride dehydrate were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the experimental water comes from the Milli-Q reference system (Millipore, America) unless stated otherwise.
Fishery product samples were Todarodes pacificus belonging to the family Ommastrephidae; Larimichthys polyactis belonging to the family Sciaenidae; Pennahia argentata belonging to the family Sciaenidae. The above three kinds of fresh fishery products were purchased in a local market (Xincheng Market, No. 95 Jindao Road, Dinghai District, Zhoushan 316021, China) and brought into the laboratory no later than 1 h in ice.
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2

Synthesis and Antioxidant Evaluation of Triisobutyryl Gallic Acids

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Triisobutyryl gallic acids were synthesized according to previous work [16 (link)]. Sodium bicarbonate, sodium phosphate dibasic dodecahydrate, sodium chloride, sodium phosphate monobasic dihydrate, anhydrous magnesium sulfate, alcohol, ethyl acetate, petroleum ether, toluene, hydrochloric acid, anhydrous ethanol, and methanol were obtained from Sinopharm Chemical Reagent (Shanghai, China). Phytol, 1-eicosanol, N,N-dicyclohexylarbodiimide (DCC), gallic acid, butylated hydroxy anisole (BHA), 2,6-di-tert-butyl-4-methylphenol (BHT), tert-butylhydroquinone (TBHQ), 2,2-diphenyl-1-picrylhydrazyl (DPPH), triethylamine, 4-dimethyaminopyridine (DMAP), hydrazinium hydrate solution (80%), glycerol trioleate, isobutyric anhydride, Tween-80, lecithin, 2,2′-azobis(2-methylpropionitrile) (AIBN), 2,2′-azobis-(2-methylpropionamidine), and dihydrochloride (AAPH) were purchased from Aladdin Reagent (Shanghai, China). Phytanol was obtained from Tokyo Chemical Industry. A thiobarbituric acid reactive substance kit was purchased from Suzhou Grace Biotechnology Co., LTD. All chemicals were of analytical grade.
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3

Enzymatic Catalysis in Biomaterials

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Citric acid and PEPA were purchased from the Aladdin Industrial Corporation (Shanghai, China). Tyrosinase (TYR) from mushrooms, DA, GSH, ascorbic acid (AA), glucose oxidase (GOD), alkaline phosphatase (ALP), glycine (Gly), lysozyme, trypsin and catalase were supplied by Sigma-Aldrich. Cysteine, acetone, sodium chloride, sodium dihydrogen phosphate dodecahydrate (Na2HPO4·12H2O), sodium phosphate monobasic dihydrate (NaH2PO4·2H2O) and glucose were obtained from Sinopharm Chemical Reagent Co., Ltd. All aqueous solutions used in this work were prepared in ultrapure water obtained from a Millipore system. All analytical grade reagents were used without any purification.
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