Lipin1

Manufactured by Cell Signaling Technology

Sourced in United States

Lipin1 is a protein that functions as a phosphatidate phosphatase enzyme, catalyzing the conversion of phosphatidic acid to diacylglycerol. It plays a role in lipid biosynthesis and metabolism.

Automatically generated - may contain errors

Lab products found in correlation

Phospho acc, by Cell Signaling Technology (2 mentions) Luteolin, by Enzo Life Sciences (1 mentions) Staurosporin, by Merck Group (1 mentions) Acsl1, by Cell Signaling Technology (1 mentions) Perchloric acid, by Merck Group (1 mentions) Sodium palmitate, by Merck Group (1 mentions) Pbabe nf2, by Addgene (1 mentions) 5 iodotubercidin, by Enzo Life Sciences (1 mentions) Gsk2194069, by Merck Group (1 mentions) Anti gapdh antibody, by Merck Group (1 mentions) Cerulenin, by Enzo Life Sciences (1 mentions) 5 tetradecyloxy 2 furoic acid tofa, by Enzo Life Sciences (1 mentions) Dimethylsulfoxyde dmso, by Merck Group (1 mentions) Acetyl coenzyme a lithium salt, by Merck Group (1 mentions) Anti fasn, by Cell Signaling Technology (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Ammonium formate, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions) Phosphor akt ser473, by Cell Signaling Technology (1 mentions)

4 protocols using lipin1

Lipid Metabolism Regulation in NF2 Cells

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pBabe-NF2 was obtained from Addgene. Anti-Fasn siRNA (M-040091-01-0005), anti-Acaca siRNA (M-063938-01-0005), anti-Mtor (M-065427-00-0005), anti-Rptor (M-058754-01-0005), anti-Rictor (M-064598-01-0005) and non-silencing (D-001206-13-05) siRNA were purchased from Dharmacon. Individual siRNAs against Mtor (SASI_Mm01-00164496 and -00164492), Rictor (-00137732 and -00137730), Rptor (-00055298 and -00334580), Fasn (-00177858 and -00177854), Acaca (-0011590 and -00115905), and Mlycd (-00028572 and -00028576) were purchased from Sigma-Aldrich. Anti-Merlin antibodies were purchased from Abcam (#ab88957). Lipid synthesis and metabolism antibody kit (includes anti-Fasn, -phospho ACC, -ACC, -Lipin1, -ACLY, -phospho ACLY, -ACSL1, and -ACECS1 antibodies), and anti-Casp3 antibodies were purchased from Cell Signaling Technology. Anti-SREBP1 antibodies were purchased from Santa Cruz Biotechnology. Anti-GAPDH antibodies were purchased from EMD-Millipore.
Cerulenin, C75, luteolin, 5-(tetradecyloxy)-2-furoic acid (TOFA) and 5-iodotubercidin were purchased from Enzo Life Sciences. GSK2194069, dimethylsulfoxyde (DMSO), staurosporin, sodium palmitate, 70% perchloric acid, ammonium formate, acetonitrile, acetyl-coenzyme A lithium salt, malonyl coenzyme A lithium salt, propionyl-coenzyme A lithium salt, and poly-L-lysine were purchased from Sigma-Aldrich.

Stepanova D.S., Semenova G., Kuo Y.M., Andrews A.J., Ammoun S., Hanemann C.O, & Chernoff J. (2017). An essential role for the tumor suppressor Merlin in regulating fatty acid synthesis. Cancer research, 77(18), 5026-5038.

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Western Blot Analysis of Metabolic Proteins

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Tissue extracts were prepared with RIPA lysis buffer (25mM Tris-HCl pH 7.6, 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) supplemented with a PhosStop phosphatase inhibitor and cOmplete^TM protease inhibitor mixture (Roche). Protein extracts were resolved by SDS PAGE. Blots were incubated overnight at 4°C with antibodies against the following proteins (source, catalog number, and dilution of the antibody are given in parentheses): IDE (Origene, TA327113, 1:1000), LIPIN1 (Cell Signaling, #14906, 1:1000), IRE1α (Cell Signaling, #3294, 1:1000) , phospho-IRE1α Ser724 (Novus Biologicals, NB100-2323, 1:1000 ), AKT (Cell Signaling, #4691, 1:2000), phosphor-AKT Ser473 (Cell Signaling, #4060, 1:1000), GSK3β (Cell Signaling, #12456, 1:2000), phospho-GSK3β Ser9 (Cell Signaling, #5558, 1:1000), GAPDH (Santa Cruz, sc-32233, 1:2000). A polyclonal rabbit anti-human TM6SF2 antibody was raised against a peptide corresponding to the C-terminal 15 amino acids of human TM6SF2 CPPPSDPLALHKKQH (YenZym Antibodies, LLC, CA). After washing, membranes were incubated with an IRDye-conjugated IgG (LI-COR Biosciences, Lincoln, NE) secondary antibody diluted 1:5000 for 1 h. The intensity of the protein bands was quantified using an image processing program (LI-COR Biosciences, Lincoln, NE).

Fan Y., Wolford B.N., Lu H., Liang W., Sun J., Zhou W., Rom O., Mahajan A., Surakka I., Graham S.E., Liu Z., Kim H., Ramdas S., Fritsche L.G., Nielsen J.B., Gabrielsen M.E., Hveem K., Yang D., Song J., Garcia-Barrio M.T., Zhang J., Liu W., Zhang K., Willer C.J, & Chen Y.E. (2021). Type 2 diabetes sex-specific effects associated with E167K coding variant in TM6SF2. iScience, 24(11), 103196.

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AMPK Signaling Pathway Analysis

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The isolated cells were lysed using RIPA buffer, and the resultant protein concentrations were determined using the bicinchoninic acid (BCA) protein assay kit (Pierce-Thermo Fisher Scientific, Waltham, MA, USA). Equal quantities of proteins were resolved on SDS-PAGE gels and transferred to nitrocellulose membranes. Membranes were blocked in TBS with 5% nonfat dry milk and 0.1% Tween 20 and probed with the indicated primary antibodies. AMPK and phospho-AMPK antibodies, lipin-1, ATP citrate lyase (ATP-CL) and phospho-ATP-CL, and acetyl-CoA carboxylase (ACC) and phospho-ACC were all purchased from Cell Signaling Technology (Danvers, MA, USA). After incubation with horseradish peroxidase-conjugated secondary antibodies, signal was visualized using Amersham ECL Prime Western blot detection reagent (GE Life Technologies, Boston, MA). Finally, densitometry and normalization were completed. Western blots are representative of at least three independent experiments.

Piktel D., Nair R.R., Rellick S.L., Geldenhuys W.J., Martin K.H., Craig M.D, & Gibson L.F. (2022). Pitavastatin Is Anti-Leukemic in a Bone Marrow Microenvironment Model of B-Lineage Acute Lymphoblastic Leukemia. Cancers, 14(11), 2681.

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Adipogenesis Regulation by Farnesol

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Farnesol was purchased from Sigma Chemicals Co. (St. Louis, MO, United States). Farnesol was dissolved in dimethyl sulfoxide (DMSO). 3-Isobutyl-1-methylxanthine (IBMX), dexamethasone (Dex), insulin, indomethacin, compound C (CC), and Oil-Red O powder were purchased from Sigma (St. Louis, MO, United States). Dulbecco’s Modified Eagle’s Medium (DMEM), penicillin/streptomycin/glutamine (P/S/G), bovine serum (BS), and fetal bovine serum (FBS) were purchased from Gibco BRL (Grand Island, NY, United States). Anti-CCAAT/enhancer binding protein α (C/EBPα), anti-UCP1, anti-peroxisome proliferator-activated receptor γ coactivator 1 α (PGC1α), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Antibodies for PPARγ, AMP-activated protein kinase alpha (AMPKα), pAMPKα, acetyl-CoA carboxylase (ACC), pACC, adiponectin, and lipin1 were purchased from Cell Signaling Technology (Beverly, MA, United States).

Kim H.L., Jung Y., Park J., Youn D.H., Kang J., Lim S., Lee B.S., Jeong M.Y., Choe S.K., Park R., Ahn K.S, & Um J.Y. (2017). Farnesol Has an Anti-obesity Effect in High-Fat Diet-Induced Obese Mice and Induces the Development of Beige Adipocytes in Human Adipose Tissue Derived-Mesenchymal Stem Cells. Frontiers in Pharmacology, 8, 654.

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