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Mtesr 1 media gentle cell dissociation reagent

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MTeSR-1 is a gentle cell dissociation reagent designed for use with human embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is a proprietary formulation that facilitates the dissociation of cell clusters into single cells without compromising cell viability or pluripotency.

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Lab products found in correlation

Megascript t7 kit, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Mini protean tgx gels 4 20, by Bio-Rad (2 mentions) Matrigel, by BD (2 mentions) Megaclear kit, by Thermo Fisher Scientific (2 mentions) Purelink genomic dna kit, by Thermo Fisher Scientific (2 mentions) Oligonucleotide, by Integrated DNA Technologies (2 mentions) Trypsin, by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions) Dmem media, by Thermo Fisher Scientific (2 mentions) Sodium silicate, by Merck Group (2 mentions) Phusion high fidelity dna polymerase, by New England Biolabs (2 mentions) Pageblue solution, by Thermo Fisher Scientific (2 mentions) Cardiotoxin c9759, by Merck Group (2 mentions) Emd millipore amicon ultra 4 100kda, by Merck Group (2 mentions)

Spelling variants of this product

MTeSR media (56 citations) Gentle Dissociation Reagent (10 citations) Cell dissociation reagent (5 citations) Gentle cell dissociation reagents (3 citations) Gentle Cell Dissociation media (2 citations)

2 protocols using mtesr 1 media gentle cell dissociation reagent

1

Synthesis and Characterization of Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oligonucleotides were purchased from Integrated DNA Technologies (IDT, Coralville, IA). Gold nanoparticles (15 nm) were purchased from BBI solutions. Sodium citrate and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonate (HEPES) were purchased from Mandel Scientific (Guelph, ON). Sodium silicate and cardiotoxin (C9759) were purchased from Sigma Aldrich (St. Louis, MO). Phusion High-fidelity DNA Polymerase was purchased from NEB (Ipswich, MA). The MEGAscript T7 kit, the MEGAclear kit, the PageBlue solution, the propidium iodide, and the PureLink genomic DNA kit were purchased from Thermo Fischer (Waltham, MA). Mini-PROTEAN TGX Gels (4–20%) were purchased from Bio-Rad (Hercules, CA). MTeSR-1 media gentle cell dissociation reagent was purchased from StemCell Technologies (Vancouver, Canada). Matrigel was purchased from BD Biosciences (San Jose, CA). DMEM media, non-essential amino acids, penicillin-streptomycin, DPBS and 0.05% trypsin were purchased from Life Technologies (Carlsbad, CA). EMD Millipore Amicon Ultra-4 100kDa was purchased from Millipore (Germany).
Lee K., Conboy M., Park H.M., Jiang F., Kim H.J., Dewitt M.A., Mackley V.A., Chang K., Rao A., Skinner C., Shobha T., Mehdipour M., Liu H., Huang W.C., Lan F., Bray N.L., Li S., Corn J.E., Kataoka K., Doudna J.A., Conboy I, & Murthy N. (2017). Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair. Nature biomedical engineering, 1, 889-901.
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2

Synthesis and Characterization of Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oligonucleotides were purchased from Integrated DNA Technologies (IDT, Coralville, IA). Gold nanoparticles (15 nm) were purchased from BBI solutions. Sodium citrate and 4-(2-hydroxyethyl) piperazine-1-ethanesulfonate (HEPES) were purchased from Mandel Scientific (Guelph, ON). Sodium silicate and cardiotoxin (C9759) were purchased from Sigma Aldrich (St. Louis, MO). Phusion High-fidelity DNA Polymerase was purchased from NEB (Ipswich, MA). The MEGAscript T7 kit, the MEGAclear kit, the PageBlue solution, the propidium iodide, and the PureLink genomic DNA kit were purchased from Thermo Fischer (Waltham, MA). Mini-PROTEAN TGX Gels (4–20%) were purchased from Bio-Rad (Hercules, CA). MTeSR-1 media gentle cell dissociation reagent was purchased from StemCell Technologies (Vancouver, Canada). Matrigel was purchased from BD Biosciences (San Jose, CA). DMEM media, non-essential amino acids, penicillin-streptomycin, DPBS and 0.05% trypsin were purchased from Life Technologies (Carlsbad, CA). EMD Millipore Amicon Ultra-4 100kDa was purchased from Millipore (Germany).
Lee K., Conboy M., Park H.M., Jiang F., Kim H.J., Dewitt M.A., Mackley V.A., Chang K., Rao A., Skinner C., Shobha T., Mehdipour M., Liu H., Huang W.C., Lan F., Bray N.L., Li S., Corn J.E., Kataoka K., Doudna J.A., Conboy I, & Murthy N. (2017). Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair. Nature biomedical engineering, 1, 889-901.
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