Camptothecin

Primary human meningioma cells were plated into 96-well dishes (10,000 cells/ well), grown for two days in 20 % FCS-supplemented DMEM, washed in 37 °C-thermostatted 0.5 % FCS-supplemented DMEM for three times (20 min, respectively), and stimulated in the same medium with 50 μg/ml camptothecin (Sigma Aldrich, Steinheim, Germany), 10 nM CXCL16 (Pepro Tech) or with combination of both for up to 24 h. In parallel, control wells without/with stimulation in DMSO were used. For detection of active caspase-3 amounts, samples were washed in PBS and incubated in 100 μl Homogeneous Caspase 3/7 substrate (Apo-ONE® Homogeneous Caspase-3/7 Assay; Promega, Madison, USA) for 30 min according to the manufacturer’s instruction and as described before [15 (link)]. The amounts of active caspase-3 were determined in relation to a caspase 7 standard (Enzo Life Science, Lörrach, Germany), and camptothecin-stimulated wells were set 100 %.

Embryonal rat cortical neurons (RCN) were derived as previously described from rat E15-16 cortices^{35 (link)}. For each experiment, cortices were obtained from all embryos (mixed, unknown sex) of a single pregnant Sprague-Dawley® dam (Envigo). After dissociation, cells were seeded onto 96-well, 12-well, 60 mm, 100 mm cell culture plates (Corning, Corning, NY) or XF24 cell culture microplates (Agilent, Santa Clara, CA) and maintained in serum-free conditions using the B27 supplement (ThermoFisher, Waltham, MA) as described previously^{35 (link)}. We previously showed these cultures are >90% neuronal³⁶ .
Etoposide (Cat. #BML-GR307, Enzo Life Sciences, Farmingdale, NY), staurosporine (Cat. #ALX-380-014, Enzo Life Sciences, Farmingdale, NY), camptothecin (Cat. #ALX-350-015, Enzo Life Sciences, Farmingdale, NY), C₂-ceramide (Cat. #BML-SL100, Enzo Life Sciences, Farmingdale, NY), doxorubicin (Cat. #CST-5927, Cell Signaling Technologies, Danvers, MA), and mithramycin (Cat. #11434, Cayman Chemical Company, Ann Arbor, MI) were used to treat 7 days in vitro (DIV) cells at concentrations and for times indicated elsewhere.

The following chemicals were used: 4-hydroxytamoxifen (4OHT), Doxycycline, Trypan Blue, Aphidicolin, Doxorubicin, BrdU (Sigma-Aldrich, Germany), Camptothecin (Enzo Life Sciences Farmingdale, NY USA), poly (I:C)-HMW/-LMW (InvivoGen, Toulouse, France). Antibodies used are provided in Table S5.