Elan drc 2 icp ms instrument

Manufactured by PerkinElmer

Sourced in Switzerland

The Elan DRC II ICP-MS is an inductively coupled plasma mass spectrometry (ICP-MS) instrument manufactured by PerkinElmer. It is a highly sensitive analytical tool designed for the detection and quantification of trace elements in a variety of sample types.

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Lab products found in correlation

Hydrochloric acid (hcl), by Merck Group (1 mentions) Elan drcii axial field technology, by PerkinElmer (1 mentions) At261 delta range analytical balance, by Mettler Toledo (1 mentions)

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ELAN DRC II (123 citations) ICP-MS (87 citations) ELAN DRC II ICP-MS (25 citations)

7 protocols using elan drc 2 icp ms instrument

Trace Metal Quantification in Tissues

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Tissues of interest were collected, weight and dried until their weight remained constant. Samples were digested in concentrated nitric acid (1 mL) for 2 days. Samples were completed to a total volume of 8 mL with water. Indium was added as an internal standard at a concentration of 0.5 ppb. Determination of the metal content was achieved using an Elan DRC II ICP-MS instrument (Perkin-Elmer, Switzerland) equipped with a Meinhard nebulizer and a cyclonic spray chamber. The ICP-MS instrument was tuned using a solution containing 1 ppb of the following elements: Mg, In, Ce, Ba, Pb and U. External standards were prepared gravimetrically in an identical matrix to the samples with single element standards obtained from CPI International (Amsterdam, The Netherlands). Values were normalized per mg of wet tissue.

Clavel C.M., Nowak-Sliwinska P., Păunescu E., Griffioen A.W, & Dyson P.J. (2015). In vivo evaluation of small-molecule thermoresponsive anticancer drugs potentiated by hyperthermia. Chemical Science, 6(5), 2795-2801.

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Determination of Molybdenum in Serum

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The presence of Mo in the aforementioned serum samples was measured by ICP-MS using standard methodologies. Briefly, serum was digested overnight in 10 volumes of 9:1 9 M HNO₃ : 5 M HCl (Sigma-Aldrich) at room temperature in tubes pre-washed with HNO₃ and rinsed with ultra-pure water (17.45 MΩ-cm). The next day, samples were boiled for 30 minutes and allowed to cool. A total of 100 μl of each sample was transferred to new, pre-washed screw-top tubes and diluted 1:1 with ultra-pure water. Samples were then submitted to the Analytical Spectroscopy Service Laboratory at North Carolina State University for analysis on an Elan DRCII ICP-MS instrument (Perkin Elmer) for the presence of Mo.

Brady D.C., Crowe M.S., Greenberg D.N, & Counter C.M. (2017). Copper Chelation Inhibits BRAFV600E-driven Melanomagenesis and Counters Resistance to BRAFV600E and MEK1/2 Inhibitors. Cancer research, 77(22), 6240-6252.

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Quantification of Ruthenium and Platinum in Tumor Samples

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Ruthenium and platinum content in explanted MSTO-211H tumors from different treatment groups was assessed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The tumor samples were digested with 400 µL of 69% HNO₃ for 24 h at room temperature and adjusted with ultrapure water to a final volume of 4 mL. Indium was added as an internal standard at a concentration of 1ppb. External standards were prepared gravimetrically in an identical matrix to the samples (with regard to internal standard and nitric acid) with single element standards. Ru, Pt and In standard solutions (1gL⁻¹ in 2% HNO₃, 2% HNO₃ and 10% HCl, respectively) were purchased from CPI International (Amsterdam, The Netherlands). Determinations of total metal contents were achieved on an Elan DRC II ICP-MS instrument (Perkin Elmer, Switzerland) equipped with a Meinhard nebulizer and a cyclonic spray chamber.

Riedel T., Cavin S., van den Bergh H., Krueger T., Liaudet L., Ris H.B., Dyson P.J, & Perentes J.Y. (2018). Chemo-manipulation of tumor blood vessels by a metal-based anticancer complex enhances antitumor therapy. Scientific Reports, 8, 10263.

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Trace Element Analysis in Serum

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Concentrations of copper, selenium and zinc were determined using the inductively coupled plasma dynamic reaction cell MS (ICP-DRC-MS) method detailed in the CDC Laboratory Procedure Manual [16 -18 (link)]. Replicates were separate 1:30 dilutions of serum with diluent (140 nmol/L gallium, as an IS in 2% v/v nitric acid, 5% v/v ethyl alcohol and 0.01% v/v Triton^™ X-100 in≥18.2 M·Ω water). Replicates were tested by introducing an aerosol of each dilution to an ELAN DRC II ICP-MS instrument (PerkinElmer, Waltham, MA) using an SC4-DX (Elemental Scientific Inc., Omaha, NE) autosampler. External calibrators were prepared in pooled serum using SI-traceable standard SM-2107-013 (High-Purity Standards, Charleston, SC). All elements were measured in DRC mode (using ammonia gas) to eliminate polyatomic interferences. Two custom-made, characterized serum bench quality control materials were inserted at the beginning and end of each analytical run, and the multi-rule quality control system (MRQCS) developed by Caudill et al. [19 (link)], was used to determine if runs were in control.

Boggs A.S., Kilpatrick L.E., Burdette C.Q., Tevis D.S., Fultz Z.A., Nelson M.A., Jarrett J.M., Kemp J.V., Singh R.J., Grebe S.K., Wise S.A., Kassim B.L, & Long S.E. (2020). Development of a pregnancy-specific reference material for thyroid biomarkers, vitamin D, and nutritional trace elements in serum. Clinical chemistry and laboratory medicine, 59(4), 671-679.

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Quantification of Cellular Metal Uptake

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Cells were seeded in 6-well cell culture plates with either 5 × 10⁵ (ECRF24 and A2780cisR) or 3 × 10⁵ (A2780) cells per well and allowed to adhere overnight. Subsequently, cells were treated with either medium (CTRL), single drug treatment of RAPTA-C or the combination of erlotinib/RAPTA-C and incubated for 5 h. Cells were then washed 3 times with ice cold phosphate-buffered saline (PBS) and detached using PBS-Based Enzyme Free-Cell Dissociation Solution (Millipore™ Specialty Medium, Cat. # S-014-B). Next, cell lysis was achieved using the ‘freeze-thaw’ method, i.e. by performing 5 cycles of cooling the cells at −20 °C for 20 min and warming the cells at +37 °C for 20 min. The cell lysate was then stored at −20 °C until the day of the analysis.
Ten μl of each sample was isolated for protein quantification using the Quick Start™ Bradford Protein Assay based on the manufacturer’s instructions. The samples were subsequently digested for 3 h at 37 °C by adding 150 μl of 65% nitric acid. Following digestion, each sample was transferred to a 15 mL tube and diluted with water containing (1 ppb indium as an internal standard) to a final solution containing 3% nitric acid. ICP-MS was performed using an Elan DRC II ICP-MS instrument (Perkin Elmer, Switzerland) equipped with a Meinhard nebulizer and a cyclonic spray chamber.

Berndsen R.H., Weiss A., Abdul U.K., Wong T.J., Meraldi P., Griffioen A.W., Dyson P.J, & Nowak-Sliwinska P. (2017). Combination of ruthenium(II)-arene complex [Ru(η6-p-cymene)Cl2(pta)] (RAPTA-C) and the epidermal growth factor receptor inhibitor erlotinib results in efficient angiostatic and antitumor activity. Scientific Reports, 7, 43005.

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Aluminum Determination in Samples by ICP-MS

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The aluminum contents in the samples were determined utilizing Inductively Coupled Plasma Mass Spectrometry (ICP-MS) with Elan DRC II Axial Field Technology from PERKIN ELMER Inc., USA. This study utilized an ELAN DRC II ICP-MS instrument (PerkinElmer, Concord, Ontario, Canada) equipped with Dynamic Reaction Cell (DRC). The instrument was supplied with a platinum cone, a quartz injector (2.0 mm orifice), a PC3 spray chamber, and a PFA nebulizer. The ICP-MS system was operated under the following conditions: argon gas flow rate-17 L/min, nebulizer gas flow rate- 0.92 L/min, argon auxiliary gas flow rate- 1.2 L/min, integration time- 0.3 s, sample uptake rate- 1.0 ml/min and RF power-1200 W, Cell gas (NH₃) flow rate-0.5 ml/min, and the sample introduction gas (O₂) flow rate- 30 ml/min.
The apparatus was calibrated using the standard solution (Perkin Elmer, Concord, Ontario, Canada) supplied by the manufacturer. The concentration of metal contaminants in each sample was determined in triplicate.

Aljohar H.I., Nawawi R.A., Albanyan N.S., Aljarboa S, & Orfali R.S. (2023). An Assessment of aluminum contamination in neonatal parenteral nutrition solutions based on measured versus labeled content. Saudi Pharmaceutical Journal : SPJ, 32(2), 101941.

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Arsenic Speciation Analysis by ICP-MS

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An Agilent (Santa Clara, CA, USA) model 8800 triple quadrupole (QQQ) inductively coupled plasma-mass spectrometer (ICP-MS) was used for the determination of total arsenic in sample extracts. A Mettler model AT261 Delta range analytical balance was used in the gravimetric preparation of samples and standards. A CEM (Matthews, NC, USA) Multiwave 3000 microwave digestion system equipped with EasyPrep TFM microwave vessels was used for sample digestion. Advanced vortex mixer and Isotemp oven model 737 F from Fisher Scientific were used for sample extraction. A Perkin-Elmer (Shelton, CT, USA) LC system coupled to a Perkin-Elmer model Elan DRCII ICP-MS instrument operating in standard mode was used for arsenic speciation analysis. The LC system consisted of a Peltier-cooled Series 200 autosampler and a Series 200 quaternary pump with a 50 μL injection loop. A Hamilton PRP-X 100 (250 mm × 4.6 mm, 10 μm) anion exchange column (Reno, NV, USA) and a Macherey-Nagel Nucleosil 100–5 SA (250 mm × 4 mm, 5 μm) cation exchange column (Bethlehem, PA, USA) were used for the separation of arsenic species. The mobile phase composition and chromatographic method details are outlined in Table 1.

Luvonga C., Rimmer C.A., Yu L.L, & Lee S.B. (2020). Determination of total arsenic and hydrophilic arsenic species in seafood. Journal of food composition and analysis : an official publication of the United Nations University, International Network of Food Data Systems, 96(103729), 10.1016/j.jfca.2020.103729.

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