C2C12 and HEK293T cells were obtained from the American Type Culture Collection (ATTC, Masnassas, Virginia) and analyzed for potential myoplasma contamination every two month using the PCR Mycoplasma Test Kit I/C from Promocell (Heidelberg, Germany). Identity of C2C12 cells was authenticated by induction of myotube differentiation. Antibodies recognizing BRAF, EEA1, LAMIN A/C and MYOGENIN were obtained from BD Biosciences (San Jose, California) while PAX3, MYOD and myosin heavy chain antibodies (MHC) were from the Developmental Studies Hybridoma bank (Iowa City, Iowa). Additional PAX3 and anti-LBX1 antibodies were purchased from Abcam (Cambridge, Massachusetts). Anti-phospho-PAX3 (Serine 205) was a kind gift of Dr. A. D. Hollenbach (Miller et al., 2008 (link)). The antibody against CD31 and recombinant Scatter Factor/Hepatocyte Growth factor (HGF) was obtained from R and D Systems (Minneapolis, Minnesota). Anti-phospho BRAF, anti-phospho ERK1/2 and anti-GAPDH antibodies were purchased from Cell Signaling (Danvers, Massachusetts). Anti-MYF5 was from Santa Cruz (Dallas, Texas) and Anti-GST was from GE healthcare (Chicago, Illinois).
Anti phospho braf
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho BRAF is a laboratory reagent used to detect the phosphorylated form of the BRAF protein. BRAF is a serine/threonine-protein kinase involved in the MAP kinase/ERK signaling pathway. The anti-phospho BRAF antibody can be used to study the activation and regulation of the BRAF protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti gst, by GE Healthcare (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Lamin a c, by BD (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Myogenin, by BD (1 mentions)
Anti myf5, by Santa Cruz Biotechnology (1 mentions)
Anti phospho erk1 2, by Cell Signaling Technology (1 mentions)
Pcr mycoplasma test kit 1 c, by PromoCell (1 mentions)
Anti 4e bp1, by Cell Signaling Technology (1 mentions)
Anti b raf, by Cell Signaling Technology (1 mentions)
Phospho akt thr308 rabbit mab, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Phospho 4ebp1, by Cell Signaling Technology (1 mentions)
2 protocols using anti phospho braf
1
Characterization of C2C12 and HEK293T Cells
2
Western Blot Assay of Signaling Pathways
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The Western blots were carried out according to the standard protocol. The following primary antibodies were used: phospho‐Akt (Thr308) rabbit mAb (13038), phospho‐Akt rabbit mAb (Ser 473) (4060), anti‐Akt (pan) rabbit mAb (4691), phospho‐P70s6k rabbit mAb (9205), P70s6k rabbit mAb (2708), phospho‐4ebp1 (#13396, monoclonal), anti‐4ebp1 (#9644), anti‐phospho‐B‐Raf (#2696, polyclonal), anti‐B‐Raf (#9433, monoclonal), anti‐phospho‐Erk (#4370, monoclonal), anti‐Erk (#4695, monoclonal) (all from Cell Signaling Technology, Beverly, MA, USA). The band density was quantified using ImageJ software (NIH, Rockville, MD, USA).
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