Protein extraction and Western blotting experiments were performed as described previously [12 (link)]. Primary antibodies used were as follow: anti-TGF-β1, CDK4, c-Myc, CyclinD3, p27, phospho-Akt, Akt, phospho-Erk1/2, Erk1/2, phospho-JNK, JNK, phospho-p38, p38, phospho-Smad 2, Smad 2/3, Smad 4, and Histone H3 (Cell Signaling, Danvers, MA); anti-CD63, CD81, and CD9 (Santa Cruz, Santa Cruz, CA); and anti-β-actin (Sigma-Aldrich). HRP-conjugated horse anti-mouse and goat anti-rabbit IgG (Cell Signaling) were used as secondary antibodies.
Hrp conjugated goat anti rabbit and horse anti mouse igg
Manufactured by Cell Signaling Technology
Sourced in United States
HRP-conjugated goat anti-rabbit and horse anti-mouse IgG are secondary antibodies used in immunodetection techniques. They are conjugated with horseradish peroxidase (HRP) enzyme, which can catalyze a colorimetric or chemiluminescent reaction for signal detection.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (3 mentions)
C myc, by Cell Signaling Technology (3 mentions)
Phospho smad2, by Cell Signaling Technology (2 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
Ptbp1, by Cell Signaling Technology (2 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Histone h3, by Cell Signaling Technology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Phospho p38, by Cell Signaling Technology (1 mentions)
Cyclin d3, by Cell Signaling Technology (1 mentions)
Smad2 3, by Cell Signaling Technology (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Anti tgf β1, by Cell Signaling Technology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Smad4, by Cell Signaling Technology (1 mentions)
Anti cd63, by Santa Cruz Biotechnology (1 mentions)
Grp94, by Abcam (1 mentions)
Anti tgf β1, by Abcam (1 mentions)
Ecl detection system, by Thermo Fisher Scientific (1 mentions)
Ecl advance reagent, by GE Healthcare (1 mentions)
8 protocols using hrp conjugated goat anti rabbit and horse anti mouse igg
1
Protein Extraction and Western Blotting
2
Protein Extraction and Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein extraction and Western blotting experiments were performed as described previously [32 (link)]. Primary antibodies used were as follow: anti-TGF-β1, CD63, ALIX, GRP94, CAIX (Abcam, Cambridge, MA), phospho-Smad 2, phospho-Smad 3, Smad 2/3, (Cell Signaling, Danvers, MA); and anti-β-actin, GAPDH (Sigma-Aldrich, St Louis, MO). HRP-conjugated horse anti-mouse and goat anti-rabbit IgG (Cell Signaling, Danvers, MA) were used as secondary antibodies.
3
Protein Isolation and Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein isolation from HUVECs and Western blotting experiments were performed according to the previous description.24 (link) Primary antibody (anti-LPAR2, anti-CD63, anti-CD9, anti-HPV18 E6, and anti-HPV18 E7) was purchased from Abcam (Cambridge, UK). Secondary antibody (HRP-conjugated horse anti-mouse and goat antirabbit IgG) were purchased from Cell Signaling. Protein bands were detected with ECL Advance reagent (GE Healthcare Biosciences, Buckinghamshire, UK) using the ECL detection system (Thermo Scientific, Waltham, MA, USA).
4
Western Blotting Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein extraction and Western blotting experiments were performed as described in our previous reports [9 (link)] [10 (link)]. Primary antibodies used were as follow: anti-socs7 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); antibodies against PARP, pAkt, Akt, pErk1/2, Erk1/2, c-Myc, E-cadherin, pβ-catenin, β-catenin, cateninδ-1, and GAPDH (Cell Signaling Technology, Inc., Danvers, MA, USA); and anti-FSCN1 (Abcam, Cambridge, UK). HRP-conjugated goat anti-rabbit and horse anti-mouse IgG (Cell Signaling Technology) were used as secondary antibodies. GAPDH served as an internal control.
5
Western Blotting for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein extraction and Western blotting analysis were performed as described in previous reports [29 (link),32 (link)]. The following primary antibodies were used: antibodies against c-Myc, PTBP1, p-AKT, AKT, p-ERK, ERK, PARP, and GAPDH (Cell Signaling Technology, Inc., Danvers, MA, USA), PKM1, and PKM2 (Novus Biologicals, Littleton, CO, USA) and FSCN1 (Abcam, Cambridge, UK). HRP-conjugated goat anti-rabbit and horse anti-mouse IgG (Cell Signaling Technology) were used as secondary antibodies. GAPDH served as an internal control.
6
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein extraction and Western blot analysis were performed as described in previous reports [36 (link),37 (link)]. The following primary antibodies were used: anti-FGFR2 and anti-HER2 (Cell Signaling Technology, Inc., Danvers, MA, USA), anti-DDX6 (RCK) (Santa Cruz Biotechnology (Dallas, TX, USA); sc-376433 K1414), and anti-β-actin (Sigma-Aldrich, St. Louis, MO, USA). HRP-conjugated goat anti-rabbit and horse anti-mouse IgG (Cell Signaling Technology, Danvers, MA, USA) were used as secondary antibodies. β-actin served as an internal control.
We used the web-available soft Image J in the case of calculating densitometric values.
We used the web-available soft Image J in the case of calculating densitometric values.
7
Protein Analysis by Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein extraction and Western blotting experiments were performed as described in our previous publications [9 (link), 49 (link)]. The following primary antibodies were used: antibodies against KLF4 (Cell Signaling Technology, Inc., Danvers, MA, USA and Proteintech Group, Inc, Rosemont, IL, USA), c-Myc, PTBP1, PARP, LC3B, p-PKM2, GAPDH (Cell Signaling Technology, Inc., Danvers, MA, USA), PKM1, and PKM2 (Novus Biologicals, USA). HRP-conjugated goat anti-rabbit and horse anti-mouse IgG (Cell Signaling Technology) were used as secondary antibodies. GAPDH served as an internal control.
8
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At 48 h after transfection, protein samples were extracted from the cells and western blot analysis was performed following the protocols described previously (25 (link),26 (link)). The materials used for the analysis were as follows: RIPA buffer (Thermo Fisher Scientific, Inc.), Protease Inhibitor Cocktail (Sigma-Aldrich), DC Protein assay kit (Bio-Rad), polyacrylamide gels (Wako Pure Chemical), PVDF membrane (Bio-Rad), and PVDF Blocking Reagent for Can Get Signal® (TOYOBO). The primary antibodies used were anti-GA (Abcam; EP7212), anti-LDHA (Abcam; EP1566Y), and anti-β-actin (Sigma-Aldrich; A2228). The secondary antibodies used were HRP-conjugated goat anti-rabbit and horse anti-mouse IgG (Cell Signaling Technology). The immunoblots were detected and visualized by Fusion-FX7 (Vilber Lourmat) with LuminataTM Forte Western HRP Substrate (Millipore). β-actin was used as an internal control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!