For ANA analysis, serum was diluted 1:5 in PBS/0.2% BSA and incubated on Kallestad® HEp-2 Slides (Bio-rad). Slides were washed and stained with anti–mouse IgG FITC (SouthernBiotech). Slides were mounted with Aqua PolyMount and analyzed on a fluorescent microscope (Axio Observer.Z1). Images were obtained using a Zeiss Axioplan 2 microscope with a Zeiss Axiocam digital camera (Zeiss).
Anti mouse igg fitc
Manufactured by Southern Biotech
Sourced in United States
Anti–mouse IgG FITC is a fluorescently-labeled secondary antibody that binds to mouse immunoglobulin G (IgG). It is commonly used in various immunoassays and microscopy techniques to detect and visualize mouse primary antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Kallestad hep 2 slides, by Bio-Rad (2 mentions)
Axiocam digital, by Zeiss (2 mentions)
Oct compound, by Sakura Finetek (2 mentions)
Rabbit anti cd3, by Abcam (1 mentions)
Streptavidin cy5, by Thermo Fisher Scientific (1 mentions)
Rhodamine tritc conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Bz x700, by Keyence (1 mentions)
Mouse anti e cadherin, by BD (1 mentions)
Fitc anti mouse b220, by BioLegend (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Bz 9000, by Keyence (1 mentions)
4 protocols using anti mouse igg fitc
1
Serum ANA Immunofluorescence Assay
2
Fluorescent Staining of Murine Lymphoid Tissues
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For fluorescent staining, mouse lymph nodes and kidneys were removed after transcardial perfusion with PBS and then frozen with liquid nitrogen after being embedded in OCT compound (Sakura Finetek Japan, Tokyo, Japan). Four-micrometer cryostat sections were fixed in acetone and stained with anti-mouse IgG FITC (Southern Biotech, Birmingham, USA), anti-mouse complement C3 (MP Biomedicals, Santa Ana, CA, USA), rat anti-CD11b (Tonbo Biosciences, San Diego, CA, USA), rabbit-anti-CD3 (Abcam, Cambridge, UK) or biotin-conjugated rat anti-CD45R/B220 (BioLegend, San Diego, CA, USA). For multiple staining, primary antibodies were followed by AF 488-conjugated anti-rat IgG (Jackson ImmunoResearch, West Grove, PA, USA), rhodamine (TRITC)-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch) and Cy5-streptavidin (Thermo Fisher Scientific, Waltham, MA, USA). Staining of sections was visualized with a fluorescence microscope (BZ-X700; Keyence, Osaka, Japan).
3
Serum ANA Immunofluorescence Assay
4
Immunofluorescence Staining of Intestinal Tissues
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Freshly isolated normal intestinal tissues and tumor tissues were snap-frozen in OCT compound (Sakura Finetek) and 5 to 6-μm-thick sections were prepared using a cryostat. After drying at room temperature, sections were fixed in acetone for 10 min and then allowed to dry. Nonspecific binding was blocked with 3% BSA/PBS for 30 min at room temperature, and then the slides were washed and stained for 1 h at room temperature with mouse anti-E-Cadherin (BD Transduction Laboratories, 610182) or supernatants of iGB cell culture or mouse sera, appropriately diluted as indicated. After washing, the slides were stained for 1 h at room temperature with Alexa Fluor® 660 goat anti-mouse IgG(H+L) (Invitrogen, A21054) and anti-mouse B220-FITC (BioLegend, RA3-6B2) or anti-mouse IgG-FITC (Southern Biotech, 1030–02), respectively. After washing, slides were mounted with the fluorescent mounting medium (ProLong™ Gold antifade reagent with DAPI, Invitrogen) and examined using an immunofluorescence microscope (BZ-9000; Keyence).
The fixed sections were also stained with haematoxylin and eosin (H&E) through gradual changes of alcohols for dehydration.
The fixed sections were also stained with haematoxylin and eosin (H&E) through gradual changes of alcohols for dehydration.
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