Aeroset automatic analyzer

In the DFTJ cohort, peripheral venous blood samples were collected after overnight fasting. Plasma glucose levels were measured with Aeroset automatic analyzer (by glucose oxidase method; Abbott Laboratories. Abbott Park, Illinois, USA). The serum bilirubin, lipids, hepatic function, and renal function were measured by the ARCHITECTCi8200 automatic analyzer (ABBOTT Laboratories. Abbott Park, Illinois, USA). In the SCHS, morning random blood samples were collected, and plasma lipids, hepatic enzymes, and bilirubin were measured via colorimetric method on a chemistry analyzer (AU5800 Analyzer, Beckman Coulter, Brea, CA).

Sociodemographic characteristics including sex, age, education (primary school or below, junior high school, senior high school, college or above), and marital status (married, unmarried, widowed or divorced) were collected from the questionnaire replies. We also obtained lifestyle characteristics including physical activity, smoking and alcohol drinking status from the questionnaires. We obtained reproductive data including menopause status, abortion, the use of contraceptives, and the use of hormone replacement therapy, which were self-reported from the questionnaires. Peripheral venous blood samples were collected after overnight fasting, and plasma glucose levels were measured with Aeroset automatic analyzer (by glucose oxidase method; Abbott Laboratories. Abbott Park, Illinois, USA). We defined diabetes mellitus as fasting plasma glucose ≥7.0 mmol/L, self-reported physician diagnosis of diabetes mellitus, or current use of antidiabetic medications. Similarly, hypertension was defined as a self-reported previous diagnosis of hypertension, taking antihypertensive treatment, or systolic blood pressure >140 mmHg or diastolic blood pressure >90 mmHg.