Linomat

Manufactured by CAMAG

Sourced in Switzerland, Germany

The Linomat is a laboratory instrument used for the automated application of samples onto thin-layer chromatography (TLC) plates. It is designed to deposit precise and reproducible sample bands or spots, ensuring consistent and reliable results in TLC analysis.

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Lab products found in correlation

Hptlc system, by CAMAG (1 mentions) Tlc silica gel plate, by Merck Group (1 mentions) Scanner 3, by CAMAG (1 mentions) Reprostar 3, by CAMAG (1 mentions) C18 column, by Waters Corporation (1 mentions) Boeravinone b, by Merck Group (1 mentions) Rutin, by Merck Group (1 mentions) Silica gel gf254, by Merck Group (1 mentions) Direct yellow 59, by Merck Group (1 mentions) Dataanalysis, by Bruker (1 mentions) Amazon sl mass spectrometer, by Bruker (1 mentions) Trap control, by Bruker (1 mentions)

Spelling variants of this product

Linomat 5 (149 citations) Linomat V sample applicator (37 citations) Linomat V Automatic Sample Spotter (7 citations) Linomat IV (7 citations) Linomat V automatic sample applicator (4 citations) Linomat autosampler (3 citations) Linomat-4 autosampler (2 citations) Linomat-5 TLC Sampler (2 citations) Linomat 5 TLC applicator (2 citations) Linomat V semi-automatic applicator (2 citations)

7 protocols using linomat

HPTLC Analysis of Medicinal Plant Extract

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The HPTLC was performed at the Radiant Research Laboratories Private Limited, Bangalore. The analysis was carried out by application of the sample and standard dissolved in methanol on HPTLC plate’s silica gel 60 F254 (20 × 10 cm). Spots of extract (6 and 9 μg/l) and standard (3, 6 and 9 and 12 μg/l) were applied on the plates. The PL leaves extract (47.5 mg) and standard (5.16 mg) were dissolved in (ratio of 1:10 in terms of weight in mg) 10ml of the solvent. Scanning of the developed plates was carried out at 333 nm (before) and 550 nm (after) with following details:

Instrument: CAMAG Linomat 5

Linomat 5 application parameters

Spray gas: Inert gas

Sample solvent type: Methanol

Dosage speed: 150 nl/s

Predosage volume: 0.2 μl

Sequence

Syringe size: 100 μl

Number of tracks: 10

Application position Y: 12.0 mm

Band length: 8.0 mm

Calibration parameters

Calibration mode: Single level

Statistics mode: CV

Evaluation mode: Area and peak height

Formula:

Doshi G.M., Zine S.P., Chaskar P.K, & Une H.D. (2014). Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites. Pharmacognosy Research, 6(3), 234-239.

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HPTLC identification of curcumin and calebin-A

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The preliminary identification of curcumin and calebin-A was performed by using HPTLC system (Camag, Muttens, Switzerland) comprising of Camag Linomate V semiautomatic sample applicator and Linomat syringe (100 μl). The stationary phase was TLC silica gel plates (Merck Millipore, 60 F₂₅₄) where, 2 μl of each samples were loaded and developed using solvent system chloroform:methanol (98:2). Using scanner 3 (Camag), the plate was scanned at 280 nm with deuterium illumination. The images were captured on Camag reprostar 3 with win CATS software (ver. 1.4.3.6336) and compared the R_f value with the standard calebin-A.

Majeed A., Majeed M., Thajuddin N., Arumugam S., Ali F., Beede K., Adams S.J, & Gnanamani M. (2019). Bioconversion of curcumin into calebin-A by the endophytic fungus Ovatospora brasiliensis EPE-10 MTCC 25236 associated with Curcuma caesia. AMB Express, 9, 79.

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TLC-HPLC Characterization of Medicinal Herbs

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Chromatographic separation was achieved on TLC plates (20 × 20 cm) and precoated with silica gel 60 F254 (Merck, India) of 0.2 mm thickness. Samples were spotted using CAMAG Linomat and Sample Spotter (Camag, Switzerland) equipped with a 100 μL syringe. Plates were developed in a glass chamber, and the experimental conditions were maintained at 20° ± 5°C.
A. paniculata and B. diffusa were run in dichloromethane : hexane : methanol (7 : 2 : 1) solvent system, P. niruri by dichloromethane : acetic acid : methanol (12 : 0.5 : 2) system, and T. purpurea by acetone : petroleum ether (4 : 6) system to identify the extent of bioactive compounds along with commercial standard marker compounds. Pure Andrographolide, Boeravinone B, Phyllanthin, and Rutin were procured from Sigma-Aldrich, USA. The TLC of the marker compounds showed major single spots, whereas the extracts and formulation showed many additional spots with all different Rf values. They were further standardized and characterized by HPLC along with standard commercial markers of the respective bioactive molecules. HPLC system and C-18 columns were from Waters Inc, USA. Methanol: water and acetonitrile: water were used to run the systems, respectively. All solvents were analytical grade and procured from Merck, India.

Dey D., Chaskar S., Bhatt N, & Chitre D. (2020). Hepatoprotective Activity of BV-7310, a Proprietary Herbal Formulation of Phyllanthus niruri, Tephrosia purpurea, Boerhavia diffusa, and Andrographis paniculata, in Alcohol-Induced HepG2 Cells and Alcohol plus a Haloalkane, CCl4, Induced Liver Damage in Rats. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 6428906.

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Extraction and Identification of Fungal IAA

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Extraction of IAA was carried out from 10-day-old fermented broth of isolated endophytic fungi. Culture supernatant was acidified to pH 2.0 using HCl (1 N) and extracted twice with double volumes of ethyl acetate. Ethyl acetate fractions were collected and evaporated in a rotary evaporator at 40°C. Extract was dissolved in minimum volume of methanol and used for analysis. Extracted fungal IAA (2 μl) was applied on silica gel coated plate (Silica gel G f254, thickness 0.25 mm, Merck®, Berlin, Germany) via LINOMAT (CAMAG, Darmstadt, Germany) and run in a solvent system of n-butanol : ammonia : water (10 : 1 : 10 v/v/v). At last, TLC plate was sprayed with Salkowski reagent to visualize IAA band. Standard IAA was also applied on to the plate to identify fungal IAA on the basis of Rf value.

Adnan M., Alshammari E., Ashraf S.A., Patel K., Lad K, & Patel M. (2018). Physiological and Molecular Characterization of Biosurfactant Producing Endophytic Fungi Xylaria regalis from the Cones of Thuja plicata as a Potent Plant Growth Promoter with Its Potential Application. BioMed Research International, 2018, 7362148.

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Quantitative TLC Analysis of Serum Drugs

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Aliquots of SER.DMP and DMP stock standard solutions (1 mg/mL) equivalent to (5–25 μg/spot) were transferred to a set of 10-mL volumetric flasks and the volume was then completed to the mark with methanol. A 10 μL of each solution were applied to TLC plate (10 cm × 10 cm) using Camag Lino mat auto sampler with micro syringe (100 μL). The plate was then developed by the ascending technique using methanol: chloroform (70:30 v/v) as a mobile phase. The plate was then removed and air-dried. The chromatogram was scanned at 220 nm. Calibration curves representing the relationship between integrated peak area and the corresponding concentration in μg/spot of SER.DMP and DMP were plotted.

Kelani K.M., Nassar A.M., Omran G.A., Morshedy S., Elsonbaty A, & Talaat W. (2023). Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment. BMC Chemistry, 17(1), 76.

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Lipid Profiling by HPTLC-ESI-MS

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Lipid extracts (20 µL) were automatically spotted onto an HPTLC glass back plate (Merck KGaA, Darmstadt, Germany) by using a Linomat (CAMAG, Muttenz, Switzerland) and separated with chloroform/ethanol/water/triethylamine (30:35:7:35, by vol.) as the mobile phase. After air-drying for 15 min, the HPTLC plate was dipped into primuline solution (Direct Yellow 59, Sigma-Aldrich, Taufkirchen, Germany, 50 mg/l in acetone/water (80:20, by vol.)). Lipids were visualized under UV light (366 nm) and spots were marked with a pencil. The lipids in each spot were automatically eluted by a Plate Express™ TLC plate reader (Advion, Ithaca, NY, USA) with methanol as solvent and directly analyzed by electrospray ionization mass spectrometry (ESI MS) on an Amazon SL mass spectrometer (Bruker Daltonics GmbH, Bremen, Germany) with the following settings: Spray voltage 4.5 kV, end plate offset 500 V, nebulizer gas 7 psi, drying gas (N₂) 3 L/min, capillary temperature 200 °C. For data acquisition and subsequent analysis of lipid spectra, the software “Trap Control” and “Data Analysis” version 4.1 (Bruker Daltonics GmbH) were used, respectively.

Biadglegne F., Schmidt J.R., Engel K.M., Lehmann J., Lehmann R.T., Reinert A., König B., Schiller J., Kalkhof S, & Sack U. (2022). Mycobacterium tuberculosis Affects Protein and Lipid Content of Circulating Exosomes in Infected Patients Depending on Tuberculosis Disease State. Biomedicines, 10(4), 783.

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Carbohydrate Separation and Identification by HP-TLC

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High-performance thin-layer chromatography (HP-TLC) was used to separate and identify carbohydrates by molecular weight. The HP-TLC was carried out using a methodology proposed by Alvarado et al. [40 ]. Five microliters of samples (20 mg/mL) were put in a pre-coated HP-TLC plate using a sample applicator (CAMAG Linomat, 5 Muttenz, Switzerland) with a 100 L syringe (Hamilton, Bonaduz, Switzerland). An HP-TLC plate nano-sil NH2/UV254 amino-modified was used as the stationary phase (Macherey-Nagel GmbH and Co. KG, Duren, Germany). The mobile phase consisted of n-butanol:methanol:water:acetic acid mixture (50/25/20/1; v/v/v/v). The plate was atomised with an ethanol/H₂SO₄/anisaldehyde solution (18/1/1; v/v/v) and revealed at 190 °C for 20 min. After chromatography, densitometric scanning was performed using a TLC Scanner 3 (CAMAG Linomat) at 500 nm. Wincats Software v1.4.4.6337 (CAMAG Linomat) operated the scanner. The radiation source was a D2&W lamp, and the scanning speed was 20 mm/s.

Rodríguez-Rodríguez R., Barajas-Álvarez P., Morales-Hernández N., Camacho-Ruíz R.M, & Espinosa-Andrews H. (2022). Physical Properties and Prebiotic Activities (Lactobacillus spp.) of Gelatine-Based Gels Formulated with Agave Fructans and Agave Syrups as Sucrose and Glucose Substitutes. Molecules, 27(15), 4902.

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