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Formononetin

Manufactured by Tokyo Chemical Industry
Sourced in Japan

Formononetin is a chemical compound that serves as a laboratory reagent. It functions as a phytoestrogen, a naturally occurring plant compound with estrogen-like properties. This information is presented in a factual and unbiased manner, without extrapolation on its intended use.

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2 protocols using formononetin

1

Purification and Characterization of YjiC

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Genistein, daidzein, biochanin A and formononetin were purchased from Tokyo Chemical Industry (Japan). UDP-α-D-glucose was purchased from Sigma-Aldrich (USA). Genistein 7-O-β-D-glucoside (genistin) was available in our laboratory. All other chemicals and reagents were of the highest chemical grade available. The high resolution mass spectrometry spectra were obtained in positive ion mode on ACQUITY (UPLC, Waters Corp., USA) coupled with SYNAPT G2-S (Waters Corp.). The details of the methodology for the cloning, expression, and purification of YjiC are described in our previous report (Pandey et al., 2013a (link)).
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2

Streptozotocin-Induced Diabetic Rat Model

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Streptozotocin was purchased from Sigma-Aldrich (St. Louis, MO, United States). Formononetin (98.8% pure) was purchased from Tokyo Chemical Industry Co., Ltd. (TCI), Japan. Insulin ELISA assay kit was purchased from RayBiotech Inc., Norcross, GA, United States. Diagnostic kits for estimation of glucose, triglyceride, cholesterol, HDL, LDL, and glycated hemoglobin were purchased from Transasia Biomedicals Ltd., India. SIRT1 antibody (B-7) was purchased from Santa Cruz biotechnology, United States. Casein was purchases from Clarion Casein Limited, Kadi, India. Cholesterol and DL-methionine were procured from Research Lab Fine Chem Industries, Mumbai, India.
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