Anti robo1 ab7279

Manufactured by Abcam

Sourced in United States

Anti-Robo1 (ab7279) is a primary antibody that targets the Robo1 protein. Robo1 is a receptor involved in axon guidance and cell migration. This antibody can be used in applications such as Western blot and immunohistochemistry to detect and study the Robo1 protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti akt 4685, by Cell Signaling Technology (1 mentions) Anti p erk1 2, by Santa Cruz Biotechnology (1 mentions) Anti p tyr, by Santa Cruz Biotechnology (1 mentions) Anti p akt ser473, by Cell Signaling Technology (1 mentions) Anti erk1 2 9102, by Cell Signaling Technology (1 mentions) Vegf c, by ProSpec (1 mentions) Cck 8, by Beyotime (1 mentions) Tunel kit, by Roche (1 mentions) Luciferase reporter assay, by Promega (1 mentions)

Spelling variants of this product

Anti-Robo1 (8 citations) Ab7279 (6 citations) ROBO1 (6 citations)

2 protocols using anti robo1 ab7279

Investigating VEGFR-Robo Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

VEGF-C and Slit2N were purchased from ProSpec (East Brunswick, NJ) and
PreproTech, Inc. (Rocky Hill, NJ), respectively. Antibodies used include
anti-VEGFR-3, anti-p-Tyr, anti-GAPDH, and anti-p-ERK1/2 (Santa Cruz
Biotechnology, Santa Cruz, CA); anti-p-Akt (Ser-473), anti-VEGFR-2 (55B11),
anti-p-VEGFR-2 (Tyr1175), anti-ERK1/2 (9102), and anti-Akt (4685) (Cell
Signaling Technology, Beverly, MA); anti-PI3K p85 (Upstate Biotechnology,
Waltham, MA), anti-Robo1 (ab7279), and anti-Robo4 (ab10547) (Abcam Inc.,
Cambridge, MA).

Yu J., Zhang X., Kuzontkoski P.M., Jiang S., Zhu W., Li D.Y, & Groopman J.E. (2014). Slit2N and Robo4 regulate lymphangiogenesis through the VEGF-C/VEGFR-3 pathway. Cell Communication and Signaling : CCS, 12, 25.

+ Open protocol

+ Expand

Molecular Mechanisms of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell experiments, including cell culture, cell transfection, luciferase reporter assays (Promega, USA), western blotting (anti-ROBO1: ab7279; dilution: 1:500; Abcam) and CCK8 assays (Beyotime), were carried out as described previously [8 (link)]. miR-Com-, miR-152-3p-, sh-Con-, sh-ROBO1- and ROBO1-overexpressing plasmids were obtained from GenePharma (Shanghai, China). A TUNEL kit (Roche) was utilized to analyse cell apoptosis.

Yin T, & Zhao H. (2022). miR-152-3p impedes the malignant phenotypes of hepatocellular carcinoma by repressing roundabout guidance receptor 1. Cellular & Molecular Biology Letters, 27, 22.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!