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2 protocols using anti integrin α8

1

Immunofluorescence Staining of ECM Proteins

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Anti-α-actinin-4 was from Santa Cruz Biotechnology, Inc (Dallas, TX, USA, Cat #: SC-49333); anti-CD11b was from CedarLane Laboratories Limited (Hornby, Ontario, Canada, Cat #: CL8941AP); anti-Fibronectin was from Sigma (St. Louis, MO, USA, Cat #: F3648); anti-Integrin α8 was from R&D Systems (Minneapolis, MN, USA, Cat #: AF4076); anti-Laminin α1 was a gift from Dr. Dale Abrahamson (KU Medical Center, Kansas City, KS, rat monoclonal 8B3); anti-Laminin α2 and anti-β actin were from Sigma (St. Louis, MO, USA, Cat #: L0663); anti-Laminin α5 was a gift from Dr. Jeff Miner (Washington University, St. Louis, MO); anti-p-FAK397 was from Assay Biotechnology (Sunnyvale, CA, USA, Cat #: A0925) and from Invitrogen (Carlslab, CA); anti-Total FAK was from Cell Signaling Technology (Danvers, MA, USA, Cat #: 3285). Anti-MMP-10 antibodies were from Millipore (Billerica, MA, USA, Cat # ABT 289). All Alexa-fluor conjugated secondary antibodies were from Invitrogen (Carlsbad, CA), including donkey anti-rat 488, donkey anti-rabbit 555, goat anti-rat 488, goat anti-rabbit 555, donkey anti-rabbit 488, and donkey anti-goat 568. The small molecular inhibitor for FAK activation, TAE226 was from Chem Scene (Monmouth Junction, NJ, Cat #CS-0594); the peptide inhibitor for NF-kappaB (SN-50) was from Calbiochem (now EMD Millipore, Billerica, MA, Cat #481480)
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2

Immunohistochemical Staining for Cell Markers

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Anti-α-actinin 4 was from Santa Cruz Biotechnology, Inc (Dallas, TX, USA, Cat #: SC-49333); anti-CD31 was from Thermo Fisher Scientific, Inc (Rockford, IL, USA, Cat #: MA1-40074); Anti-collagen 1 was from CedarLane (Cat# CL50151AP) at 1:200 and CD45 was from eBioscience (Cat# 14-0451) at 1:100. anti-Endothelin-1 was from Abbiotec (San Diego, CA, USA, Cat #: 250633) and Pierce (Lifetechnologies, Cat# PA3.067); anti-Endothelin Receptor A was from Novus Biologicals (Littleton, CO, USA, Cat #: NB600-836); anti-Endothelin Receptor B was from EMD Millipore (Billerica, MA, USA, Cat #: AB3284); anti-Integrin α8 was from R&D Systems, Inc (Minneapolis, MN, USA, Cat #: AF4076); anti- Laminin α2 was from Sigma-Aldrich (St. Louis, MO, USA, Cat #: L-0663); anti-Laminin α5 was a gift from Dr. Jeff Miner (Washington University, St. Louis, MO).
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