Rabbit anti gst pi

Manufactured by Enzo Life Sciences

Rabbit anti-GST-pi is an affinity-purified polyclonal antibody raised in rabbits against the glutathione S-transferase pi (GST-pi) protein. It is designed for the detection and quantification of GST-pi in various applications.

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Lab products found in correlation

Rat anti ki67, by Thermo Fisher Scientific (1 mentions) Mouse anti mcm2, by BD (1 mentions) Goat anti egfr, by R&D Systems (1 mentions) Mouse anti gfap, by Merck Group (1 mentions) Mouse anti neun, by Merck Group (1 mentions) Rat anti brdu, by Abcam (1 mentions) Mouse anti tyrosine hydroxylase, by Merck Group (1 mentions) Rabbit anti β catenin, by Merck Group (1 mentions) Guinea pig anti dcx, by Merck Group (1 mentions) Rabbit anti calretinin, by Merck Group (1 mentions) Rabbit anti s100β, by Agilent Technologies (1 mentions) Rabbit anti calbindin, by Merck Group (1 mentions) Mouse anti acetylated tubulin, by Merck Group (1 mentions) Rabbit anti gfap, by Merck Group (1 mentions) Rabbit anti mog, by Abcam (1 mentions) Alexa fluor labeled secondary antibodies, by Jackson ImmunoResearch (1 mentions) Rabbit anti s100b, by Merck Group (1 mentions) Goat anti sox10, by Santa Cruz Biotechnology (1 mentions) Mouse anti aqp4, by Santa Cruz Biotechnology (1 mentions) Goat anti iba1, by Abcam (1 mentions)

2 protocols using rabbit anti gst pi

Immunohistochemical Analysis of SVZ and Olfactory Bulb

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Brains were fixed overnight at 4°C in 4% PFA in PBS, then cryoprotected in 30% sucrose in PBS for 1–3 days at 4°C, then frozen in OCT or cryogel on dry ice after 3–12 hr equilibration at 4°C. Sections were cut at 12 μm thickness spanning the rostral half of the SVZ (typically 6 sections per slide) or the entire olfactory bulb (typically 8 sections per slide). Sections were immunostained with the following primary antibodies: rat anti-BrdU (Abcam clone Bu1/75, 1/500, after heat-mediated antigen retrieval), guinea pig anti-Dcx (1/1000; Millipore), mouse anti-Mcm2 (BD Biosciences, 1/500, after heat-mediated antigen retrieval), rat anti-Ki67 (1/500; eBioscience), mouse anti-tyrosine hydroxylase (1/1000; Millipore), rabbit anti-calretinin (1/1000; Sigma), rabbit anti-calbindin (1/500; Millipore), rabbit anti-S100β (1/1000; Dako), rabbit anti-GST-pi (1/3000; Enzo, Farmingdale, NY), and mouse anti-NeuN (1/1000; Millipore). Fixed whole-mount SVZs were stained with mouse anti-acetylated tubulin (1/1000; Sigma), rabbit anti-β-catenin (1/500; Sigma), mouse anti-GFAP (1/3000; Sigma), and goat anti-EGFR (1/250; R&D Systems). Alexa Fluor 488-, 555-, and 647-conjugated secondary antibodies were used (Life Technologies, Carlsbad, CA).

Mich J.K., Signer R.A., Nakada D., Pineda A., Burgess R.J., Vue T.Y., Johnson J.E, & Morrison S.J. (2014). Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain. eLife, 3, e02669.

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Cerebellar Slice Immunohistochemistry Protocol

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After treatment, cerebellar slices were rinsed twice with PBS and fixed in 4% paraformaldehyde in PBS for 20 min at room temperature. For immunohistochemistry, slices were rinsed in PBS and permeabilized in 1.5% or 10% (myelin proteins) Triton X-100 in PBS for 20 min. Slices were rinsed, blocked with 5% normal donkey serum (NDS) in PBS with 0.3% Triton X-100 for 1 h, and incubated with primary antibodies overnight at room temperature. Following 3 washes in PBS, Alexa Fluor-labeled secondary antibodies (Jackson ImmunoResearch, West Grove, PA) were applied (1:800) overnight at room temperature, washed 3 times in PBS and mounted in Fluoromount G (Southern Biotech, Birmingham, AL). The following primary antibodies were used: goat anti-Sox10 (Santa Cruz), rat anti-PLP (Yamamura, Konola, Wekerle, & Lees, 1991 (link)), mouse anti-AQP4 (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-S100b (Sigma), rabbit anti-GST-pi (Enzo), mouse anti-Caspr (NeuroMAB), mouse anti-CC1 (Calbiochem), rabbit anti-GFAP (Sigma), rabbit anti-MOG (Abcam, Cambridge, United Kingdom), mouse anti-MBP (Covance, Princeton, NJ), chicken anti- Neurofilament-H (Neuromics, Minneapolis, MN), goat anti-Iba1 (Abcam).

Liu Y., Given K.S., Owens G.P., Macklin W.B, & Bennett J.L. (2018). Distinct Patterns of Glia Repair and Remyelination in Antibody-mediated Demyelination Models of Multiple Sclerosis and Neuromyelitis Optica. Glia, 66(12), 2575-2588.

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