Log In Sign up
The largest database of trusted experimental protocols

P rb s795

Manufactured by Cell Signaling Technology
Visit Supplier

P-RB (S795) is a primary antibody that detects the phosphorylation of retinoblastoma protein (Rb) at serine 795. This post-translational modification is involved in cell cycle regulation and proliferation. The antibody can be used to analyze the phosphorylation status of Rb in various cell and tissue samples.

Automatically generated - may contain errors

Lab products found in correlation

Abemaciclib, by Selleck Chemicals (1 mentions) Ribociclib, by Selleck Chemicals (1 mentions) Rb 554136, by BD (1 mentions) Palbociclib, by Selleck Chemicals (1 mentions) Gapdh, by Abcam (1 mentions) Mycro3, by MedChemExpress (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) β actin, by Abcam (1 mentions) Palbociclib, by MedChemExpress (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Cyclin d1, by Merck Group (1 mentions) Total rb, by Cell Signaling Technology (1 mentions) Cav 1, by BD (1 mentions) Cyclin d3, by Santa Cruz Biotechnology (1 mentions) Prb s780, by Cell Signaling Technology (1 mentions) Vinculin, by Abcam (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Antibodies against cyclin D2 (D52F9) and p-RB (S795) (2 citations)

3 protocols using p rb s795

1

Cell Cycle Regulators Profiling

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Palbociclib (S1116), abemaciclib (S7158), and ribociclib (S7440) were purchased from Selleck Chemicals. CR-1–31-B and silvestrol were synthesized as described previously (26 (link), 27 (link)). Antibodies against HSP90 (H-114), Cyclin D1 (M20), Cyclin D3 (DCS28), Cyclin A2 (BF683), Cyclin E1 (HE12), Cyclin E2 (A-9), CDK2 (D-12), CDK4 (DCS-35), CDK6 (C-21), p16 (C-20), p21 (H164), and p27 (C-19) were purchased from Santa Cruz Biotechnology; p-RB (S795) and Cyclin D2 (D52F9) were purchased from Cell Signaling Technology; RB (554136) was purchased from BD Pharmingen; and eIF4A1 (ab31217) was purchased from Abcam.
Kong T., Xue Y., Cencic R., Zhu X., Monast A., Fu Z., Pilon V., Sangwan V., Guiot M.C., Foulkes W.D., Porco JA J.r., Park M., Pelletier J, & Huang S. (2019). eIF4A Inhibitors Suppress Cell-Cycle Feedback Response and Acquired Resistance to CDK4/6 Inhibition in Cancer. Molecular cancer therapeutics, 18(11), 2158-2170.
+ Open protocol
+ Expand
2

Investigating Cell Cycle Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
For this study, we purchased the following antibodies against CCNA1 (Abcam, ab172317), CDC25A (Abcam, ab92892), E2F2 (Abcam, ab138515), E2F3 (Abcam, ab50917), c-myc (Abcam, ab39688), pRb (S795) (Cell Signaling Technologies, #9301), Rb (Abcam, ab181616), E2F1 (Abcam, ab179445), E-cadherin (Cell Signaling Technologies, #8834), Vimentin (Abcam, ab92547), cyclin E1 (Abcam, ab21142), CDK6 (Abcam, ab151247), Tubulin (Abcam, ab6160), β-actin (Abcam, ab8226), Ki-67 (Cell Signaling Technologies, #9449), mouse IgG (Cell Signaling Technologies, #7076), rabbit IgG (Cell Signaling Technologies, #7074), and GAPDH (Abcam, ab181603). Palbociclib and mycro-3 were obtained from MedChem Express, and both compounds were diluted in DMSO.
Ji W., Zhang W., Wang X., Shi Y., Yang F., Xie H., Zhou W., Wang S, & Guan X. (2020). c-myc regulates the sensitivity of breast cancer cells to palbociclib via c-myc/miR-29b-3p/CDK6 axis. Cell Death & Disease, 11(9), 760.
+ Open protocol
+ Expand
3

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates were extracted in 1x Cell Lysis Buffer (Cell Signaling) supplemented with EDTA-free protease inhibitors and PhosSTOP phosphatase inhibitors (Roche). Western immunoblotting was performed using standard techniques. Primary antibodies utilized included GAPDH (Santa Cruz, sc-137179), Vinculin (Abcam, ab18058), CDK4 (Thermo Fisher Scientific, MS-469-P0), cyclin D1 (EMD Millipore, 04-1151), CAV1 (BD Transduction Lab, 610493), cyclin D3 (Santa Cruz, sc-182), pRb S780 (Cell Signaling, 9307), pRb S795 (Cell Signaling, 9301), and total Rb (Cell Signaling, 9309).
Kennedy A.L., Vallurupalli M., Chen L., Crompton B., Cowley G., Vazquez F., Weir B.A., Tsherniak A., Parasuraman S., Kim S., Alexe G, & Stegmaier K. (2015). Functional, chemical genomic, and super-enhancer screening identify sensitivity to cyclin D1/CDK4 pathway inhibition in Ewing sarcoma. Oncotarget, 6(30), 30178-30193.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!